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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
07/04/2010 |
Data da última atualização: |
07/04/2010 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
FARIAS, T. A.; ARAUJO, F. R.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; SOUZA, I. I. F.; OLIVEIRA, R. H. M. de; AZAMBUJA, A.; ROSINHA, G. M. S.; SOARES, C. O.; ELISEI, C. |
Afiliação: |
Thaís A. Farias, UFMS; FLABIO RIBEIRO ARAUJO, CNPGC; Ana Luíza A. R. Osório, UFMS; Kláudia S. G. Jorge, UFMS; Carlos A. N. Ramos, CNPGC; Ingrid I. F. Souza, CNPGC; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; Alexandre Azambuja, UFMS; GRACIA MARIA SOARES ROSINHA, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; CARINA ELISEI, CNPGC. |
Título: |
ELISA based on recombinant MPB70 to detect antibodies to Mycobacterium bovis. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009. |
Páginas: |
1 p. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Inglês |
Conteúdo: |
Bovine tuberculosis is an important infectious disease, caused by Mycobacterium bovis. The gene that encodes for MPB70 protein is present in the genome of M. bovis and has high specificity for the M. tuberculosis complex, representing a potential antigen for the diagnosis of bovine tuberculosis. The objective of this work was to develop an ELISA using recombinant protein MPB70 of M. bovis in order to detect antibodies against these bacteria in cattle. The gene mpb70 was amplified by PCR and cloned into plasmid pGEM-T Easy. After digestion with the Eco RI enzyme, the gene was subcloned into the expression plasmid pGEX-3X. The expression of the gene was verified by SDS-PAGE and Western blot with anti-glutathione-S-transferase. After standardization of the ELISA, serum samples of positive (53), negative (37) or inconclusive (47) cattle in the tuberculin test were tested. In SDS-PAGE, a recombinant protein of approximately 48 kDa was detected, which corresponds to the estimated molecular weight of GST/MPB70. In Western blot, recombinant MPB70, fused to GST, was recognized by anti-GST antibody, confirming the expression of the gene. For the ELISA with recombinant MPB70, the sensitivity and specificity found were 88.7% and 94.6%, respectively. The use of a serological test such as ELISA with recombinant MPB70, together with the cellular tests, may solve some of the problems found in the control programs of bovine tuberculosis, like inconclusive results and the lack of detection of anergic animals in advanced stages of the disease. MenosBovine tuberculosis is an important infectious disease, caused by Mycobacterium bovis. The gene that encodes for MPB70 protein is present in the genome of M. bovis and has high specificity for the M. tuberculosis complex, representing a potential antigen for the diagnosis of bovine tuberculosis. The objective of this work was to develop an ELISA using recombinant protein MPB70 of M. bovis in order to detect antibodies against these bacteria in cattle. The gene mpb70 was amplified by PCR and cloned into plasmid pGEM-T Easy. After digestion with the Eco RI enzyme, the gene was subcloned into the expression plasmid pGEX-3X. The expression of the gene was verified by SDS-PAGE and Western blot with anti-glutathione-S-transferase. After standardization of the ELISA, serum samples of positive (53), negative (37) or inconclusive (47) cattle in the tuberculin test were tested. In SDS-PAGE, a recombinant protein of approximately 48 kDa was detected, which corresponds to the estimated molecular weight of GST/MPB70. In Western blot, recombinant MPB70, fused to GST, was recognized by anti-GST antibody, confirming the expression of the gene. For the ELISA with recombinant MPB70, the sensitivity and specificity found were 88.7% and 94.6%, respectively. The use of a serological test such as ELISA with recombinant MPB70, together with the cellular tests, may solve some of the problems found in the control programs of bovine tuberculosis, like inconclusive results and the lack of detection of... Mostrar Tudo |
Thesagro: |
Diagnostico; Elisa; Mycobacterium Bovis; Sanidade Animal. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02453naa a2200301 a 4500 001 1663370 005 2010-04-07 008 2009 bl uuuu u00u1 u #d 100 1 $aFARIAS, T. A. 245 $aELISA based on recombinant MPB70 to detect antibodies to Mycobacterium bovis. 260 $c2009 300 $a1 p.$c1 CD-ROM. 520 $aBovine tuberculosis is an important infectious disease, caused by Mycobacterium bovis. The gene that encodes for MPB70 protein is present in the genome of M. bovis and has high specificity for the M. tuberculosis complex, representing a potential antigen for the diagnosis of bovine tuberculosis. The objective of this work was to develop an ELISA using recombinant protein MPB70 of M. bovis in order to detect antibodies against these bacteria in cattle. The gene mpb70 was amplified by PCR and cloned into plasmid pGEM-T Easy. After digestion with the Eco RI enzyme, the gene was subcloned into the expression plasmid pGEX-3X. The expression of the gene was verified by SDS-PAGE and Western blot with anti-glutathione-S-transferase. After standardization of the ELISA, serum samples of positive (53), negative (37) or inconclusive (47) cattle in the tuberculin test were tested. In SDS-PAGE, a recombinant protein of approximately 48 kDa was detected, which corresponds to the estimated molecular weight of GST/MPB70. In Western blot, recombinant MPB70, fused to GST, was recognized by anti-GST antibody, confirming the expression of the gene. For the ELISA with recombinant MPB70, the sensitivity and specificity found were 88.7% and 94.6%, respectively. The use of a serological test such as ELISA with recombinant MPB70, together with the cellular tests, may solve some of the problems found in the control programs of bovine tuberculosis, like inconclusive results and the lack of detection of anergic animals in advanced stages of the disease. 650 $aDiagnostico 650 $aElisa 650 $aMycobacterium Bovis 650 $aSanidade Animal 700 1 $aARAUJO, F. R. 700 1 $aOSÓRIO, A. L. A. R. 700 1 $aJORGE, K. S. G. 700 1 $aRAMOS, C. A. N. 700 1 $aSOUZA, I. I. F. 700 1 $aOLIVEIRA, R. H. M. de 700 1 $aAZAMBUJA, A. 700 1 $aROSINHA, G. M. S. 700 1 $aSOARES, C. O. 700 1 $aELISEI, C. 773 $tIn: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009.
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Embrapa Gado de Corte (CNPGC) |
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Registro Completo
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
09/12/2014 |
Data da última atualização: |
28/01/2015 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
COUTINHO, C. R.; GAMA, F. de C.; SILVA, G. S. P.; COELHO, R. S.; COSTA, M. L. E; SANTOS, J. O.; PARANHOS, B. A. J. |
Afiliação: |
Cristiane R. Coutinho, Bolsista FACEPE; FARAH DE CASTRO GAMA, CPATSA; Graziella de Sá Pinto Silva, UPE - Estagiária Embrapa Semiárido; Rosamara S. Coelho, Bolsista FUNARBE; Michele Luzia Egídio Costa, UPE - Estagiária da Embrapa Semiárido; Jéssica Oliveira Santos, UPE. Estagiária Embrapa Semiárido; BEATRIZ AGUIAR GIORDANO PARANHOS, CPATSA. |
Título: |
Atratividade de compostos proteicos e amônia à Ceratitis capitata (wied.) (Diptera: Tephritidae). |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 25., 2014, Goiânia. Entomologia integrada à sociedade para o desenvolvimento sustentável: anais. Goiânia: Sociedade Entomológica do Brasil, 2014. |
Idioma: |
Português |
Conteúdo: |
O emprego de semioquímicos vem se destacando no monitoramento e controle de várias pragas de importância agrícola, minimizando o uso de inseticidas. Foram avaliados compostos a base de proteína e amônia na atração de C. capitata . Os compostos utilizados foram corn steep liquor (CSL) e putrescina nas concentrações 0,25, 0,5 e 1 e acetato de a mônia a 30, 50 e 80%. |
Palavras-Chave: |
Ecologia química; Insect; Monitoramento; Mosca-das-frutas. |
Thesagro: |
Díptera; Entomologia; Inseto. |
Categoria do assunto: |
O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/113964/1/Beatriz-1175.pdf
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Marc: |
LEADER 01279nam a2200265 a 4500 001 2001956 005 2015-01-28 008 2014 bl uuuu u00u1 u #d 100 1 $aCOUTINHO, C. R. 245 $aAtratividade de compostos proteicos e amônia à Ceratitis capitata (wied.) (Diptera$bTephritidae).$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 25., 2014, Goiânia. Entomologia integrada à sociedade para o desenvolvimento sustentável: anais. Goiânia: Sociedade Entomológica do Brasil$c2014 520 $aO emprego de semioquímicos vem se destacando no monitoramento e controle de várias pragas de importância agrícola, minimizando o uso de inseticidas. Foram avaliados compostos a base de proteína e amônia na atração de C. capitata . Os compostos utilizados foram corn steep liquor (CSL) e putrescina nas concentrações 0,25, 0,5 e 1 e acetato de a mônia a 30, 50 e 80%. 650 $aDíptera 650 $aEntomologia 650 $aInseto 653 $aEcologia química 653 $aInsect 653 $aMonitoramento 653 $aMosca-das-frutas 700 1 $aGAMA, F. de C. 700 1 $aSILVA, G. S. P. 700 1 $aCOELHO, R. S. 700 1 $aCOSTA, M. L. E 700 1 $aSANTOS, J. O. 700 1 $aPARANHOS, B. A. J.
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