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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
14/09/2015 |
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Data da última atualização: |
16/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NOGUEIRA, G. F.; PIO, L. A. S.; PASQUAL, M.; AMARAL, A. L. do; CHERWINSKI-PEREIRA, J. E. |
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Afiliação: |
GABRIELA FERREIRA NOGUEIRA; LEILA APARECIDA SALLES PIO, UFLA; MOACIR PASQUAL, UFLA; ADRIANE LEITE DO AMARAL, CPATC; JONNY EVERSON SCHERWINSKI PEREIRA, CENARGEN. |
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Título: |
An approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In Vitro Cellular & Developmental Biology. Plant, v. 51, p. 220-230, 2015. |
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DOI: |
10.1007/s11627-014-9660-y |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro conservation techniques can be utilized for germplasm maintenance. However, few reports on the in vitro conservation of sugarcane species are present in the literature. The objective of this study was to subject sugarcane plants to in vitro under minimal growth conditions and to evaluate the survival, regeneration, and the monitoring of nuclear DNA content levels of the plants. Shoots from 10 sugarcane varie-ties (Saccharum spp.) were introduced into two media: MC1, consisting of half-strength Murashige and Skoog (MS) salts and 3% sorbitol, or MC2, similar to the first formulation, but additionally supplemented with 3.8 ?Mabscisicacid(ABA). |
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Thesagro: |
Cana de açúcar. |
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Thesaurus Nal: |
Flow cytometry; Sugarcane. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179938/1/Nogueira2015-Article-AnApproachOnTheInVitroMaintena.pdf
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Marc: |
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024 7 $a10.1007/s11627-014-9660-y$2DOI
100 1 $aNOGUEIRA, G. F.
245 $aAn approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry.$h[electronic resource]
260 $c2015
520 $aIn vitro conservation techniques can be utilized for germplasm maintenance. However, few reports on the in vitro conservation of sugarcane species are present in the literature. The objective of this study was to subject sugarcane plants to in vitro under minimal growth conditions and to evaluate the survival, regeneration, and the monitoring of nuclear DNA content levels of the plants. Shoots from 10 sugarcane varie-ties (Saccharum spp.) were introduced into two media: MC1, consisting of half-strength Murashige and Skoog (MS) salts and 3% sorbitol, or MC2, similar to the first formulation, but additionally supplemented with 3.8 ?Mabscisicacid(ABA).
650 $aFlow cytometry
650 $aSugarcane
650 $aCana de açúcar
700 1 $aPIO, L. A. S.
700 1 $aPASQUAL, M.
700 1 $aAMARAL, A. L. do
700 1 $aCHERWINSKI-PEREIRA, J. E.
773 $tIn Vitro Cellular & Developmental Biology. Plant$gv. 51, p. 220-230, 2015.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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