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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
08/11/2012 |
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Data da última atualização: |
24/04/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PIMENTA-MARTINS, M. G. R.; FURTADO, R. F.; HENEINE, L. G. D.; DIAS, R. S.; BORGES, M. de F.; ALVES, C. A. |
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Afiliação: |
Maria Gardenny Ribeiro Pimenta-Martins, UECE; ROSELAYNE FERRO FURTADO, CNPAT; Luiz Guilherme Dias Heneine, FUNED; Ricardo Souza Dias, FUNED; MARIA DE FATIMA BORGES, CNPAT; Carlucio Roberto Alves, UECE. |
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Título: |
Development of an amperometric immunosensor for detection of staphylococcal enterotoxin type A in cheese. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Microbiological Methods, v.91, p.138-143, 2012. |
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Idioma: |
Português |
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Conteúdo: |
This paper reports a novel electrochemical immunosensorforthe sensitive detection of staphylococcal enterotox in A (S EA ) based on self-assembly monolayer (SA M) and p rotein A i mm obilizat io n on gold electro de. Three diffe rent methods of protein A immobilization were tested: physical adsorption, cross-linking using glutaraldehyde and covalent binding after activation with N-hydroxysuccinimide (NHS)/N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) on cysteamine-modi fied gold electrode. The EDC/NHS method for protein A immobilization was selected to lead development of the biosensor. The coating steps of the surface modification were characterized by cyclic voltammetry and the biosensor response by chronoamperometry. The advantages of the immunosensor were exposed in its high sensitivity and speci ficity. The proposed amperometric immuno- sensor was successfully used for determination of SEA in contaminated and non-contaminated cheese samples with excellent responses. |
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Palavras-Chave: |
Biosensor; Staphylococcal enterotoxin A. |
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Thesaurus Nal: |
food safety. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01660naa a2200217 a 4500
001 1939204
005 2013-04-24
008 2012 bl uuuu u00u1 u #d
100 1 $aPIMENTA-MARTINS, M. G. R.
245 $aDevelopment of an amperometric immunosensor for detection of staphylococcal enterotoxin type A in cheese.
260 $c2012
520 $aThis paper reports a novel electrochemical immunosensorforthe sensitive detection of staphylococcal enterotox in A (S EA ) based on self-assembly monolayer (SA M) and p rotein A i mm obilizat io n on gold electro de. Three diffe rent methods of protein A immobilization were tested: physical adsorption, cross-linking using glutaraldehyde and covalent binding after activation with N-hydroxysuccinimide (NHS)/N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) on cysteamine-modi fied gold electrode. The EDC/NHS method for protein A immobilization was selected to lead development of the biosensor. The coating steps of the surface modification were characterized by cyclic voltammetry and the biosensor response by chronoamperometry. The advantages of the immunosensor were exposed in its high sensitivity and speci ficity. The proposed amperometric immuno- sensor was successfully used for determination of SEA in contaminated and non-contaminated cheese samples with excellent responses.
650 $afood safety
653 $aBiosensor
653 $aStaphylococcal enterotoxin A
700 1 $aFURTADO, R. F.
700 1 $aHENEINE, L. G. D.
700 1 $aDIAS, R. S.
700 1 $aBORGES, M. de F.
700 1 $aALVES, C. A.
773 $tJournal of Microbiological Methods$gv.91, p.138-143, 2012.
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