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 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Solos. |
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Data corrente: |
20/09/2021 |
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Data da última atualização: |
25/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEIXOTO, R. S.; COUTINHO, H. L. da C.; RUMJANEK, N. G.; MACRAE, A.; ROSADO, A. S. |
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Afiliação: |
R. S. PEIXOTO, UFRJ; HEITOR LUIZ DA COSTA COUTINHO, CNPS; NORMA GOUVEA RUMJANEK, CNPAB; A. MACRAE, UFRJ; A. S. ROSADO, UFRJ. |
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Título: |
Use of rpoB and 16S rRNA genes to analyse bacterial diversity of a tropical soil using PCR and DGGE. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Letters in Applied Microbiology, v. 35, n. 4, p. 316-320, Oct. 2002. |
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DOI: |
https://doi.org/10.1046/j.1472-765X.2002.01183.x |
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Idioma: |
Inglês |
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Conteúdo: |
Aim: To evaluate the rpoB gene as a biomarker for PCR-DGGE microbial analyses using soil DNA from the Cerrado, Brazil. Methods: DNA extraction from soil was followed by Polymerase Chain Reaction (PCR) amplification of rpoB and 16S rRNA genes. PCR products were compared by Denaturing Gradient Gel Electrophoresis (DGGE) to compare gene/community profiles. Results: The rpoB DGGE profiles comprised fewer bands than the 16S rDNA profiles and were easier to delineate and therefore to analyse. Comparison of the community profiles revealed that the methods were complementary. Conclusions, Significance and Impact of the Study: The gene for the beta subunit of the RNA polymerase, rpoB, is a single copy gene unlike 16S rDNA. Multiple copies of 16S rRNA genes in bacterial genomes complicate diversity assessments made from DGGE profiles. Using the rpoB gene offers a better alternative to the commonly used 16S rRNA gene for microbial community analyses based on DGGE. |
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Palavras-Chave: |
DNA extraction. |
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Thesaurus Nal: |
Polymerase chain reaction; Tropical soils. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 01684naa a2200217 a 4500
001 2134559
005 2023-08-25
008 2002 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1046/j.1472-765X.2002.01183.x$2DOI
100 1 $aPEIXOTO, R. S.
245 $aUse of rpoB and 16S rRNA genes to analyse bacterial diversity of a tropical soil using PCR and DGGE.$h[electronic resource]
260 $c2002
520 $aAim: To evaluate the rpoB gene as a biomarker for PCR-DGGE microbial analyses using soil DNA from the Cerrado, Brazil. Methods: DNA extraction from soil was followed by Polymerase Chain Reaction (PCR) amplification of rpoB and 16S rRNA genes. PCR products were compared by Denaturing Gradient Gel Electrophoresis (DGGE) to compare gene/community profiles. Results: The rpoB DGGE profiles comprised fewer bands than the 16S rDNA profiles and were easier to delineate and therefore to analyse. Comparison of the community profiles revealed that the methods were complementary. Conclusions, Significance and Impact of the Study: The gene for the beta subunit of the RNA polymerase, rpoB, is a single copy gene unlike 16S rDNA. Multiple copies of 16S rRNA genes in bacterial genomes complicate diversity assessments made from DGGE profiles. Using the rpoB gene offers a better alternative to the commonly used 16S rRNA gene for microbial community analyses based on DGGE.
650 $aPolymerase chain reaction
650 $aTropical soils
653 $aDNA extraction
700 1 $aCOUTINHO, H. L. da C.
700 1 $aRUMJANEK, N. G.
700 1 $aMACRAE, A.
700 1 $aROSADO, A. S.
773 $tLetters in Applied Microbiology$gv. 35, n. 4, p. 316-320, Oct. 2002.
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