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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
15/06/2021 |
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Data da última atualização: |
15/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SIMÕES, L. M. S.; SANTOS, A. P. C.; BOTTINO, M. P.; LIMA, E. A.; FERNANDES, U. R.; ORLANDI, R. E.; RODRIGUES, S. A. D.; CAIXETA, F. M.; ALVES, N. G.; SOUZA, J. C.; QUINTAO, C. C. R.; CAMARGO, L. S. A.; DODE, M. A. N.; SALES, J. N. S. |
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Afiliação: |
L. M. S. SIMÕES, UFLA; A. P. C. SANTOS, UFLA; M. P. BOTTINO, UFLA; E. A. LIMA, UFLA; U. R. FERNANDES, UFLA; R. E. ORLANDI, UFLA; S. A. D. RODRIGUES, UNB; F. M. CAIXETA, UNB; N. G. ALVES, UFLA; J. C. SOUZA, UFLA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARGOT ALVES NUNES DODE, Cenargen; J. N. S. SALES, UFLA. |
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Título: |
Nuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Theriogenology, v. 166, p. 90-96, 2021. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2020.12.021 |
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Idioma: |
Inglês |
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Conteúdo: |
The maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of metaphase II (P ¼ 0.001) and greater rates of degenerated (P ¼ 0.001) and parthenogenetically activated (P ¼ 0.001) oocytes. In experiment 2, the rates of polyspermy and degenerated were similar between Groups. However, the rate of fertilized oocytes was greater (P ¼ 0.05) in oocytes in the MatFol20 Group. It is concluded that oocyte in vivo maturation for 19.8 h after IFOT does not compromise the nuclear maturation kinetics and increases in vitro fertilization rates. However, the extra 10 h of intra-follicular incubation time decreased oocyte viability. MenosThe maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of met... Mostrar Tudo |
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Palavras-Chave: |
Fertilização in-vitro; IFOT; Intra-follicular. |
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Thesagro: |
Bovino; Fertilidade Animal. |
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Thesaurus Nal: |
Embryo (animal). |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03144naa a2200361 a 4500
001 2133325
005 2021-09-15
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2020.12.021$2DOI
100 1 $aSIMÕES, L. M. S.
245 $aNuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles.$h[electronic resource]
260 $c2021
520 $aThe maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of metaphase II (P ¼ 0.001) and greater rates of degenerated (P ¼ 0.001) and parthenogenetically activated (P ¼ 0.001) oocytes. In experiment 2, the rates of polyspermy and degenerated were similar between Groups. However, the rate of fertilized oocytes was greater (P ¼ 0.05) in oocytes in the MatFol20 Group. It is concluded that oocyte in vivo maturation for 19.8 h after IFOT does not compromise the nuclear maturation kinetics and increases in vitro fertilization rates. However, the extra 10 h of intra-follicular incubation time decreased oocyte viability.
650 $aEmbryo (animal)
650 $aBovino
650 $aFertilidade Animal
653 $aFertilização in-vitro
653 $aIFOT
653 $aIntra-follicular
700 1 $aSANTOS, A. P. C.
700 1 $aBOTTINO, M. P.
700 1 $aLIMA, E. A.
700 1 $aFERNANDES, U. R.
700 1 $aORLANDI, R. E.
700 1 $aRODRIGUES, S. A. D.
700 1 $aCAIXETA, F. M.
700 1 $aALVES, N. G.
700 1 $aSOUZA, J. C.
700 1 $aQUINTAO, C. C. R.
700 1 $aCAMARGO, L. S. A.
700 1 $aDODE, M. A. N.
700 1 $aSALES, J. N. S.
773 $tTheriogenology$gv. 166, p. 90-96, 2021.
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Embrapa Gado de Leite (CNPGL) |
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| 5. |  | AVILA, L. A.; TOZZI, J. P. L.; MIQUELETTO, P. B.; ARANTES, O.; CAPALBO, D. M. F.; MELO, I. S. de. Isolamento de bactérias de solo e rizosfera do algodoeiro com potencial de biodegradação da proteína cry. In: ENCONTRO NACIONAL DE MICROBIOLOGIA AMBIENTAL, 10., 2006, Goiânia, GO. [Anais...]. Goiânia, GO: UFG, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 6. | | PEREIRA, M. M.; GERN, J.; RAPOSO, N. R. B.; SOUZA, E. D. de; MATTOSO, L. H. C.; CAMARGO, L. S. de A.; LADEIRA, L. O.; BRANDÃO, H. de M. Expressão gênica de biomarcadores de estresse e multipotência em embriões mamíferos expostos à nanofibras de celulose e à nanotubos de carbono. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 7.; ESCOLA DE NANOTECNOLOGIA, 3., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação, 2013. p. 510-512 Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. CD-ROM. Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 7. | | TONINI, H.; BALDONI, A. B.; BOTELHO, S. de C. C. Estrutura e produção de frutos de castanheira-do-brasil em floresta nativa. In: FARIAS NETO, A. L. de; NASCIMENTO, A. F. do; ROSSONI, A. L.; MAGALHÃES, C. A. de S.; ITUASSU, D. R.; HOOGERHEIDE, E. S. S.; IKEDA, F. S.; FERNANDES JUNIOR, F.; FARIA, G. R.; ISERNHAGEN, I.; VENDRUSCULO, L. G.; MORALES, M. M.; CARNEVALLI, R. A. (Ed.). Embrapa Agrossilvipastoril: primeiras contribuições para o desenvolvimento de uma agropecuária sustentável. Brasília, DF: Embrapa, 2019. pt. 8, cap. 8, p. 589-594.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agrossilvipastoril; Embrapa Pecuária Sul. |
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| 8. | | MATOS, A. P. de; REINHARDT, D. H. R. C.; CUNHA, G. A. P. da; CABRAL, J. R. S.; SOUZA, L. F. da S.; SANCHES, N. F.; ALMEIDA, O. A. de. A cultura do abacaxi. 2 ed. rev. e ampl. Brasília, DF: Embrapa Informação Tecnológica, 2006. 91 p. (Coleção plantar, 49). Projeto Minibibliotecas.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 10. | | PEREIRA, M. C.; LEITE, D. L.; CASTILHO, P. M.; VIZZOTTO, M. Determinação de compostos fenólicos totais e atividade antioxidante em bulbos de genótipos de cebola (Allium cepa L.) cultivados em pelotas, RS, Brasil. In: SIMPÓSIO DE SEGURANÇA ALIMENTAR, 3., 2010, Florianópolis. Rompendo Barreiras: anais... Florianópolis: sbCTA-RS, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 11. | | LOPES, D. B.; OLIVEIRA, Y. M. M. de; SAMPAIO, M. J. A. M.; FOGACA, F. H. dos S.; MELLO, L. M. R. de; FREITAS, M. H. A. de; MELO, P. E. de; COSTA, J. R. da; COSTA, P. da; MOZZER, G. B.; DIAS, T. A. B.; COSTA, J. L. da S.; ARZABE, C.; SANTOS, A. C. C. dos; HAMMES, V. S.; PIEROZZI JUNIOR, I. Desafios e oportunidades para a Embrapa. In: HAMMES, V. S.; LOPES, D. B.; SANTOS, A. C. C. dos; COSTA, J. R. da; OLIVEIRA, Y. M. M. de (Ed.). Pesquisa e inovação agropecuária na agenda 2030: contribuições da Embrapa e parceiros. Brasília, DF: Embrapa, 2018. E-book. Cap. 3. 80-105 (Objetivos de Desenvolvimento Sustentável, 18) Ação gerencial: ODS: Internalização dos Objetivos de Desenvolvimento Sustentável e definição de estratégias de ação.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Agroindústria de Alimentos; Embrapa Amazônia Ocidental; Embrapa Florestas; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Roraima; Embrapa Unidades Centrais; Embrapa Uva e Vinho. |
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