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Registro Completo |
Biblioteca(s): |
Embrapa Agroindústria Tropical; Embrapa Gado de Leite. |
Data corrente: |
09/12/2019 |
Data da última atualização: |
09/12/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ANDRADE, A. P. C. DE; BORGES, M. DE F.; EVÂNIA ALTINA TEIXEIRA DE FIGUEIREDO; ARCURI, E. F. |
Afiliação: |
ANA PAULA COLARES DE ANDRADE, Universidade Federal do Ceará, Departamento de Engenharia de Alimentos; MARIA DE FÁTIMA BORGES, Embrapa Agroindústria Tropical, Doutora, Pesquisadora aposentada.; EVÂNIA ALTINA TEIXEIRA DE FIGUEIREDO, Universidade Federal do Ceará, Departamento de Engenharia de Alimentos; EDNA FROEDER ARCURI, CNPGL. |
Título: |
Diversity of Staphylococcus coagulase: positive and negative strains of coalho cheese and detection of enterotoxin encoding genes. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Boletim Centro de Pesquisa de Processamento de Alimentos, Curitiba, v. 36, n. 1, p. 1-9, jan./jun. 2019. |
DOI: |
doi.org/10.5380/bceppa.v36i1.57553 |
Idioma: |
Inglês |
Conteúdo: |
Three hundred samples of coalho cheese, from 15 diff erent brands, seven handmade and eight industrialized, were evaluated in relation to the contamination profi le by Staphylococcus coagulase-positive and negative and the occurrence of staphylococcal enterotoxin encoding genes. Two hundred and eight isolates of Staphylococcus sp. were subjected to phenotypic identifi cation and 95 were subjected to genotypic identifi cation through femA gene research and detection of genes (sea, seb, sec, sed, see, seg, seh, sei and sej) encoding enterotoxins, using the polymerase chain reaction technique (PCR). A total of 14 species of Staphylococcus were identifi ed, of which three were coagulase-positive and eleven negative, especially: S. aureus, S. xylosus, S. cohnni spp. cohnii, S. saprophyticus, S. epidermidis, S. hyicus, S. lentus, S. sciuri, S. cohnii spp. urealyticus, S. haemolyticus, S. chromogenes, S. lugdunensis, S. hominis e S. intermedius. In all the samples of handmade coalho cheese there was a prevalence of S. aureus; while at industrial samples S. xylosus (87.5%) and S. cohnii spp cohnii (50%) were predominant. The presence of the femA gene was detected in 95% (38/40) isolates of positive Staphylococcus coagulase and 16.4% (9/55) of coagulasenegative isolates. Among the enterotoxin encoding genes evaluated, there was prevalence of the seh gene (53.2%) in coagulase-positive strains and of the sec gene (46.8%) in coagulase-negative strains. The results suggest a re-evaluation of the Brazilian microbiological standards in relation to the genus Staphylococcus in foods. MenosThree hundred samples of coalho cheese, from 15 diff erent brands, seven handmade and eight industrialized, were evaluated in relation to the contamination profi le by Staphylococcus coagulase-positive and negative and the occurrence of staphylococcal enterotoxin encoding genes. Two hundred and eight isolates of Staphylococcus sp. were subjected to phenotypic identifi cation and 95 were subjected to genotypic identifi cation through femA gene research and detection of genes (sea, seb, sec, sed, see, seg, seh, sei and sej) encoding enterotoxins, using the polymerase chain reaction technique (PCR). A total of 14 species of Staphylococcus were identifi ed, of which three were coagulase-positive and eleven negative, especially: S. aureus, S. xylosus, S. cohnni spp. cohnii, S. saprophyticus, S. epidermidis, S. hyicus, S. lentus, S. sciuri, S. cohnii spp. urealyticus, S. haemolyticus, S. chromogenes, S. lugdunensis, S. hominis e S. intermedius. In all the samples of handmade coalho cheese there was a prevalence of S. aureus; while at industrial samples S. xylosus (87.5%) and S. cohnii spp cohnii (50%) were predominant. The presence of the femA gene was detected in 95% (38/40) isolates of positive Staphylococcus coagulase and 16.4% (9/55) of coagulasenegative isolates. Among the enterotoxin encoding genes evaluated, there was prevalence of the seh gene (53.2%) in coagulase-positive strains and of the sec gene (46.8%) in coagulase-negative strains. The results suggest a re-evaluatio... Mostrar Tudo |
Palavras-Chave: |
ENTEROTOXINAS ESTAFILOCÓCICAS; ENVENENAMENTO ALIMENTAR; LACTICÍNIOS. |
Thesagro: |
Intoxicação Alimentar. |
Thesaurus Nal: |
Dairy products; Enterotoxins; Staphylococcal intoxication. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/206483/1/ART19040.pdf
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Marc: |
LEADER 02514naa a2200253 a 4500 001 2116347 005 2019-12-09 008 2019 bl uuuu u00u1 u #d 024 7 $adoi.org/10.5380/bceppa.v36i1.57553$2DOI 100 1 $aANDRADE, A. P. C. DE 245 $aDiversity of Staphylococcus coagulase$bpositive and negative strains of coalho cheese and detection of enterotoxin encoding genes.$h[electronic resource] 260 $c2019 520 $aThree hundred samples of coalho cheese, from 15 diff erent brands, seven handmade and eight industrialized, were evaluated in relation to the contamination profi le by Staphylococcus coagulase-positive and negative and the occurrence of staphylococcal enterotoxin encoding genes. Two hundred and eight isolates of Staphylococcus sp. were subjected to phenotypic identifi cation and 95 were subjected to genotypic identifi cation through femA gene research and detection of genes (sea, seb, sec, sed, see, seg, seh, sei and sej) encoding enterotoxins, using the polymerase chain reaction technique (PCR). A total of 14 species of Staphylococcus were identifi ed, of which three were coagulase-positive and eleven negative, especially: S. aureus, S. xylosus, S. cohnni spp. cohnii, S. saprophyticus, S. epidermidis, S. hyicus, S. lentus, S. sciuri, S. cohnii spp. urealyticus, S. haemolyticus, S. chromogenes, S. lugdunensis, S. hominis e S. intermedius. In all the samples of handmade coalho cheese there was a prevalence of S. aureus; while at industrial samples S. xylosus (87.5%) and S. cohnii spp cohnii (50%) were predominant. The presence of the femA gene was detected in 95% (38/40) isolates of positive Staphylococcus coagulase and 16.4% (9/55) of coagulasenegative isolates. Among the enterotoxin encoding genes evaluated, there was prevalence of the seh gene (53.2%) in coagulase-positive strains and of the sec gene (46.8%) in coagulase-negative strains. The results suggest a re-evaluation of the Brazilian microbiological standards in relation to the genus Staphylococcus in foods. 650 $aDairy products 650 $aEnterotoxins 650 $aStaphylococcal intoxication 650 $aIntoxicação Alimentar 653 $aENTEROTOXINAS ESTAFILOCÓCICAS 653 $aENVENENAMENTO ALIMENTAR 653 $aLACTICÍNIOS 700 1 $aBORGES, M. DE F. 700 1 $aEVÂNIA ALTINA TEIXEIRA DE FIGUEIREDO 700 1 $aARCURI, E. F. 773 $tBoletim Centro de Pesquisa de Processamento de Alimentos, Curitiba$gv. 36, n. 1, p. 1-9, jan./jun. 2019.
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Embrapa Agroindústria Tropical (CNPAT) |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
11/09/2023 |
Data da última atualização: |
10/05/2024 |
Tipo da produção científica: |
Autoria/Organização/Edição de Livros |
Autoria: |
JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 15., 2023, São Carlos, SP. |
Título: |
Anais... |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2023. |
Páginas: |
66 p. |
Série: |
(Embrapa Pecuária Sudeste. Eventos Técnicos & Científicos, 001). |
ISSN: |
2966-0289 |
Idioma: |
Português |
Notas: |
Organizadores: Cintia Righetti Marcondes; Daniel Souza Corrêa. |
Conteúdo: |
A décima quinta edição da 'Jornada Científica Embrapa - São Carlos' foi realizada de forma virtual no dia 22 de agosto de 2023 e manteve o evento no calendário científico do município de São Carlos, reconhecido como importante ecossistema de inovação do nosso país. |
Palavras-Chave: |
Evento; Jornada científica. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156595/1/EventosTecnicosCientificos-001.pdf
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Marc: |
LEADER 00914nam a2200181 a 4500 001 2156595 005 2024-05-10 008 2023 bl uuuu 00u1 u #d 022 $a2966-0289 100 1 $aJORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 15., 2023, São Carlos, SP. 245 $aAnais...$h[electronic resource] 260 $aSão Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação$c2023 300 $a66 p. 490 $a(Embrapa Pecuária Sudeste. Eventos Técnicos & Científicos, 001). 500 $aOrganizadores: Cintia Righetti Marcondes; Daniel Souza Corrêa. 520 $aA décima quinta edição da 'Jornada Científica Embrapa - São Carlos' foi realizada de forma virtual no dia 22 de agosto de 2023 e manteve o evento no calendário científico do município de São Carlos, reconhecido como importante ecossistema de inovação do nosso país. 653 $aEvento 653 $aJornada científica
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Embrapa Pecuária Sudeste (CPPSE) |
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