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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
17/12/1993 |
Data da última atualização: |
13/06/2014 |
Autoria: |
SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DO MUSEU PARAENSE EMILIO GOELDI, 1.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA FACULDADE DE CIÊNCIAS AGRÁRIAS DO PARÁ, 3., 1993, Belém, PA. |
Título: |
Resumos. |
Ano de publicação: |
1993 |
Fonte/Imprenta: |
Belém, PA: Museu Paraense Emilio Goeldi: FCAP, 1993. |
Páginas: |
63 p. |
Idioma: |
Português |
Palavras-Chave: |
Ciência; Forest; Science; Veterinary. |
Thesagro: |
Agronomia; Botânica; Ecologia; Engenharia Florestal; Veterinária; Zoologia. |
Thesaurus Nal: |
agronomy; botany; zoology. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00789nam a2200277 a 4500 001 1393184 005 2014-06-13 008 1993 bl uuuu u01u1 u #d 100 1 $aSEMINÁRIO DE INICIAÇÃO CIENTÍFICA DO MUSEU PARAENSE EMILIO GOELDI, 1. 245 $aResumos. 260 $aBelém, PA: Museu Paraense Emilio Goeldi: FCAP$c1993 300 $a63 p. 650 $aagronomy 650 $abotany 650 $azoology 650 $aAgronomia 650 $aBotânica 650 $aEcologia 650 $aEngenharia Florestal 650 $aVeterinária 650 $aZoologia 653 $aCiência 653 $aForest 653 $aScience 653 $aVeterinary 700 1 $aSEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA FACULDADE DE CIÊNCIAS AGRÁRIAS DO PARÁ, 3., 1993, Belém, PA.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
12/09/2014 |
Data da última atualização: |
29/04/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MÜLLER, B. S. de F.; SAKAMOTO, T.; SILVEIRA, R. D. D.; ZAMBUSSI-CARVALHO, P. F.; PEREIRA, M.; PAPPAS JUNIOR, G. J.; COSTA, M. M. do C.; GUIMARÃES, C. M.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO-BRONDANI, R. P. |
Afiliação: |
BÁRBARA SALOMÃO DE FARIA MÜLLER, BIOAGRO - UFV; TETSU SAKAMOTO, UFMG; RICARDO DIÓGENES DIAS SILVEIRA; PATRICIA FERNANDA ZAMBUSSI-CARVALHO, UFG; MARISTELA PEREIRA, UFG; GEORGIOS JOANIS PAPPAS JUNIOR, UNB; MARCOS MOTA DO CARMO COSTA, CENARGEN; CLEBER MORAIS GUIMARAES, CNPAF; WENDELL JACINTO PEREIRA; CLAUDIO BRONDANI, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
Título: |
Differentially expressed genes during flowering and grain filling in common bean (Phaseolus vulgaris) grown under drought stress conditions. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Plant Molecular Biology Reporter, Athens, v. 32, p. 438-451, 2014. |
DOI: |
10.1007/s11105-013-0651-7 |
Idioma: |
Inglês |
Conteúdo: |
Drought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed sequence tags, distributed across 67 contigs, were differentially expressed by the tolerant (37 contigs) and susceptible genotypes (30 contigs) after identification under drought conditions during the two investigated plant developmental stages. Of these differential contigs, the 13 most frequent genes were exclusive to the tolerant genotype. Based on BLAST2GO, 73 % of the gene sequences were annotated and 12 % showed mapping results, with the highest similarity rate corresponding to Glycine max (41 %). According to gene ontology functional analysis, 48 % of the sequences were attributed to cell metabolic processes. Overall, 8.3 % of the transcribed sequences exhibited similarity to transcription factors, predominantly those of the AP2-EREBP family (97.8 %). Of the target sequences validated by quantitative real-time polymerase chain reaction, most genes showed an expression level that agreed with that predicted by in silico analysis. Thus, the drought transcriptome dataset is a valuable resource on the variation in these gene sequences, offering the opportunity to identify robust molecular markers tightly linked to trait-controlling loci for use in marker-assisted breeding. MenosDrought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed sequence tags, distributed across 67 contigs, were differentially expressed by the tolerant (37 contigs) and susceptible genotypes (30 contigs) after identification under drought conditions during the two investigated plant developmental stages. Of these differential contigs, the 13 most frequent genes were exclusive to the tolerant genotype. Based on BLAST2GO, 73 % of the gene sequences were annotated and 12 % showed mapping results, with the highest similarity rate corresponding to Glycine max (41 %). According to gene ontology functional analysis, 48 % of the sequences were attributed to cell metabolic processes. Overall, 8.3 % of the transcribed sequences exhibited similarity to transcription factors, predominantly thos... Mostrar Tudo |
Thesagro: |
Deficiência hídrica; Feijão; Phaseolus vulgaris. |
Thesaurus NAL: |
Abiotic stress; Beans; Water stress. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/180004/1/Muller2014-Article-DifferentiallyExpressedGenesDu.pdf
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Marc: |
LEADER 02986naa a2200325 a 4500 001 1994817 005 2024-04-29 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1007/s11105-013-0651-7$2DOI 100 1 $aMÜLLER, B. S. de F. 245 $aDifferentially expressed genes during flowering and grain filling in common bean (Phaseolus vulgaris) grown under drought stress conditions.$h[electronic resource] 260 $c2014 520 $aDrought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed sequence tags, distributed across 67 contigs, were differentially expressed by the tolerant (37 contigs) and susceptible genotypes (30 contigs) after identification under drought conditions during the two investigated plant developmental stages. Of these differential contigs, the 13 most frequent genes were exclusive to the tolerant genotype. Based on BLAST2GO, 73 % of the gene sequences were annotated and 12 % showed mapping results, with the highest similarity rate corresponding to Glycine max (41 %). According to gene ontology functional analysis, 48 % of the sequences were attributed to cell metabolic processes. Overall, 8.3 % of the transcribed sequences exhibited similarity to transcription factors, predominantly those of the AP2-EREBP family (97.8 %). Of the target sequences validated by quantitative real-time polymerase chain reaction, most genes showed an expression level that agreed with that predicted by in silico analysis. Thus, the drought transcriptome dataset is a valuable resource on the variation in these gene sequences, offering the opportunity to identify robust molecular markers tightly linked to trait-controlling loci for use in marker-assisted breeding. 650 $aAbiotic stress 650 $aBeans 650 $aWater stress 650 $aDeficiência hídrica 650 $aFeijão 650 $aPhaseolus vulgaris 700 1 $aSAKAMOTO, T. 700 1 $aSILVEIRA, R. D. D. 700 1 $aZAMBUSSI-CARVALHO, P. F. 700 1 $aPEREIRA, M. 700 1 $aPAPPAS JUNIOR, G. J. 700 1 $aCOSTA, M. M. do C. 700 1 $aGUIMARÃES, C. M. 700 1 $aPEREIRA, W. J. 700 1 $aBRONDANI, C. 700 1 $aVIANELLO-BRONDANI, R. P. 773 $tPlant Molecular Biology Reporter, Athens$gv. 32, p. 438-451, 2014.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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