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Registro Completo |
Biblioteca(s): |
Embrapa Acre. |
Data corrente: |
07/11/2005 |
Data da última atualização: |
28/06/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
WADT, L. H. de O. |
Afiliação: |
LUCIA HELENA DE OLIVEIRA WADT, CPAF-AC. |
Título: |
Nutritional status of Eucalyptus grandis clones evaluated by critical level and DRIS methods. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Revista Árvore, Viçosa, MG, v. 28, n. 1, p. 15-20, 2004. |
ISSN: |
0100-6762 (impresso) / 1806-9088 (online) |
DOI: |
10.1590/S0100-67622004000100003 |
Idioma: |
Português |
Conteúdo: |
Nutritional status of eight 1.0 and 4.7 years old clones of Eucalyptus grandis, cultivated in a medium textured Ustults - US - and a Quartzipsamments - PS - soils, in Lençóis Paulista, São Paulo, were evaluated by the Diagnosis and Recommendation Integrated System (DRIS) and Critical Level (CL) methods. Based on multivariate discriminant analysis, the DRIS indices described the nutritional status of trees better in relation to tree age and soil type than in relation to nutrient composition. Spearman's correlation coefficients showed statistically significant relationships between volumetric tree growth and nutrients when applying DRIS indices or foliar nutrient concentrations. However, the DRIS indices indicated a lower number of trees with nutritional deficiencies, in relation to the CL method. According to the CL method, P, S, and Ca were deficient in the majority of the soils and tree age categories. By the DRIS method, Ca was the only deficient nutrient in PS soils, and appeared to be particularly limited in one-year-old trees. In conclusion, the DRIS method was more efficient than the CL method in evaluating the nutritional status of eucalyptus trees. |
Palavras-Chave: |
Análise multivariada; Análisis de multivarianza; CL method; DRIS method; Estatus nutricional; Método do Nível Crítico; Método DRIS; Nutrición de las plantas. |
Thesagro: |
Eucalipto; Eucalyptus grandis; Método estatístico; Nutrição vegetal. |
Thesaurus Nal: |
Multivariate analysis; Nutritional status; Plant nutrition. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/111849/1/11712.pdf
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Marc: |
LEADER 02213naa a2200325 a 4500 001 1502860 005 2021-06-28 008 2004 bl uuuu u00u1 u #d 022 $a0100-6762 (impresso) / 1806-9088 (online) 024 7 $a10.1590/S0100-67622004000100003$2DOI 100 1 $aWADT, L. H. de O. 245 $aNutritional status of Eucalyptus grandis clones evaluated by critical level and DRIS methods.$h[electronic resource] 260 $c2004 520 $aNutritional status of eight 1.0 and 4.7 years old clones of Eucalyptus grandis, cultivated in a medium textured Ustults - US - and a Quartzipsamments - PS - soils, in Lençóis Paulista, São Paulo, were evaluated by the Diagnosis and Recommendation Integrated System (DRIS) and Critical Level (CL) methods. Based on multivariate discriminant analysis, the DRIS indices described the nutritional status of trees better in relation to tree age and soil type than in relation to nutrient composition. Spearman's correlation coefficients showed statistically significant relationships between volumetric tree growth and nutrients when applying DRIS indices or foliar nutrient concentrations. However, the DRIS indices indicated a lower number of trees with nutritional deficiencies, in relation to the CL method. According to the CL method, P, S, and Ca were deficient in the majority of the soils and tree age categories. By the DRIS method, Ca was the only deficient nutrient in PS soils, and appeared to be particularly limited in one-year-old trees. In conclusion, the DRIS method was more efficient than the CL method in evaluating the nutritional status of eucalyptus trees. 650 $aMultivariate analysis 650 $aNutritional status 650 $aPlant nutrition 650 $aEucalipto 650 $aEucalyptus grandis 650 $aMétodo estatístico 650 $aNutrição vegetal 653 $aAnálise multivariada 653 $aAnálisis de multivarianza 653 $aCL method 653 $aDRIS method 653 $aEstatus nutricional 653 $aMétodo do Nível Crítico 653 $aMétodo DRIS 653 $aNutrición de las plantas 773 $tRevista Árvore, Viçosa, MG$gv. 28, n. 1, p. 15-20, 2004.
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Registro original: |
Embrapa Acre (CPAF-AC) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
Data corrente: |
17/03/2014 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
ARAUJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; S. FILHO, A. F.; VIDAL, C. E. S.; ROXO, E.; NISHIBE, c.; ALMEIDA, N. F.; F. JUNIOR, A. A.; SILVA, M. R.; B. NETO, J. D.; CERQUEIRA, V. D.; ZUMÁRRAGA, M.; ARAUJO, F. R. |
Afiliação: |
MARCIO ROBERTO SILVA, CNPGL. |
Título: |
Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Plos One, v. 9, n. 3, p. 1-6, 2014 |
Idioma: |
Inglês Português |
Conteúdo: |
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. MenosIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with ... Mostrar Tudo |
Palavras-Chave: |
Bovine and bubaline tuberculosis; Nested-PCR system. |
Thesaurus NAL: |
Mycobacterium abscessus. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119848/1/ARAUJO-C.-P..pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99300/1/pone.0091023-1..6-2014-Nested-PCR.pdf
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Marc: |
LEADER 02739naa a2200325 a 4500 001 2010358 005 2024-02-05 008 2014 bl uuuu u00u1 u #d 100 1 $aARAUJO, C. P. 245 $aDetection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1.$h[electronic resource] 260 $c2014 520 $aIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. 650 $aMycobacterium abscessus 653 $aBovine and bubaline tuberculosis 653 $aNested-PCR system 700 1 $aOSÓRIO, A. L. A. R. 700 1 $aJORGE, K. S. G. 700 1 $aRAMOS, C. A. N. 700 1 $aS. FILHO, A. F. 700 1 $aVIDAL, C. E. S. 700 1 $aROXO, E. 700 1 $aNISHIBE, c. 700 1 $aALMEIDA, N. F. 700 1 $aF. JUNIOR, A. A. 700 1 $aSILVA, M. R. 700 1 $aB. NETO, J. D. 700 1 $aCERQUEIRA, V. D. 700 1 $aZUMÁRRAGA, M. 700 1 $aARAUJO, F. R. 773 $tPlos One$gv. 9, n. 3, p. 1-6, 2014
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Embrapa Gado de Leite (CNPGL) |
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