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Registros recuperados : 19 | |
6. | | BIRK, J. J.; STEINER, C.; TEIXEIRA, W. G.; ZECH, W.; GLASER, B. Microbial response to charcoal amendments and fertilization of a highly weathered tropical soil. In: WOODS, W. I.; TEIXEIRA, W. G.; LEHMANN, J.; STEINER, C.; WINKLERPRINS, A.; REBELLATO, L. (Ed.). Amazonian dark earths: Wim Sombroek's vision. Berlin: Springer, 2009. p. 309-324. Biblioteca(s): Embrapa Amazônia Ocidental. |
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9. | | LEHMANN, J.; SILVA JUNIOR, J. P. da; STEINER, C.; NEHLS, T.; ZECH, W.; GLASER, B. Nutrient availability and leaching in an archaeological Anthrosol and a Ferralsol of the Central Amazon basin: fertilizer, manure and charcoal amendments. Plant and Soil, v. 249, p. 343-357, 2003. Biblioteca(s): Embrapa Amazônia Ocidental. |
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10. | | KERN, J.; GIANI, L.; TEIXEIRA, W. G.; LANZA, G.; GLASER, B. What can we learn from ancient fertile anthropic soil (Amazonian Dark Earths, shell mounds, Plaggen soil) for soil carbon sequestration? Catena, v. 172, p. 104-112, Jan. 2019. Biblioteca(s): Embrapa Solos. |
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11. | | STEINER, C.; TEIXEIRA, W. G.; LEHMANN, J.; GLASER, B.; ARRUDA, M. R. de; ZECH, W.; BLUM, W. E. H. Slash and char as alternative to slash and burn - soil charcoal amendments maintain soil fertility and establish a carbon sink. In: INTERNATIONAL AGRICHAR INITIATIVE (IAI) 2007 CONFERENCE, 2007, Terrigal, Australia. [Abstracts]. Terrigal: IAI, 2007. p. 32-33. Biblioteca(s): Embrapa Amazônia Ocidental. |
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12. | | SOMBROEK, W.; KERN, D.; RODRIGUES, T. E.; CRAVO, M. da S.; CUNHA, T. J.; WOODS, W.; GLASER, B. Terra preta and terra mulata: pre-columbian Amazon Kitchen middens and agricultural fields, their sustainability and their replication. In: SYMPOSIUM ANTHROPOGENIC FACTORS OF SOIL FORMATION, 18.; WORLD CONGRESS OF SOIL SCIENCE, 17., 2002, Bangkok. Trabalho apresentado... Bangkok, 2002. Biblioteca(s): Embrapa Amazônia Oriental. |
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13. | | SOMBROEK, W.; KERN, D. C.; RODRIGUES, T. E.; CRAVO, M. da S.; CUNHA, T. J. F.; WOODS, W.; GLASER, B. Terra Preta e Terra Mulata: suas potencialidade agrícolas, suas sustentabilidade e suas reproduções. In: TEIXEIRA, W. G.; KERN, D. C.; MADARI, B. E.; LIMA, H. N.; WOODS, I. W. (Ed.). As terras pretas de índio da Amazônia: sua caracterização e uso deste conhecimento na criação de novas áreas. Manaus: EDUA: Embrapa Amazônia Ocidental, 2010. p. 25-258. Biblioteca(s): Embrapa Semiárido. |
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14. | | ZECH, W.; CRAVO, M. da S.; LAABS, V.; PINTO, A.; LILIENFEIN, J.; GLASER, B.; TEIXEIRA, W.; LEHMANN, J. New approaches towards sustainability of land-use systems in Tropical Brazil. In: GERMAN-BRAZILIAN WORKSHOP ON NEOTROPICAL ECOSYSTEMS. ACHIEVEMENTS AND PROSPECTS OF COOPERATIVE RESEARCH, 2000, Hamburg. Proceedings... Hamburg: SHIFT: MADAM: WAVES, 2002. p. 118-119. Biblioteca(s): Embrapa Amazônia Ocidental. |
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15. | | ZECH, W.; CRAVO, M. da S.; LAABS, V.; PINTO, A.; LILIENFEIN, J.; GLASER, B.; WENCESLAU, T.; LEHMANN, J. New approaches towards sustainability of land-use systems in tropical Brazil. In: GERMAN-BRAZILIAN WORKSHOP ON NEOTROPICAL ECOSYSTEMS, 2000, Hamburg. Program and abstracts... Hamburg: University, 2000. p. 30 Biblioteca(s): Embrapa Amazônia Ocidental. |
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16. | | LEHMANN, J.; SILVA JUNIOR, J. P. da; RONDON, M.; CRAVO, M. D. S.; GREENWOOD, J.; NEHLS, T.; STEINER, C.; GLASER, B. Slash-and-char: a feasible alternative for soil fertility management in the Central Amazon? In: WORLD CONGRESS OF SOIL SCIENCE, 17., 2002, Bangkok, Thailand. Proceedings... [S.l.: s.n.], 2002. v. 2. p. 439. (WCSS. Symposium, 13). Paper n. 449. Biblioteca(s): Embrapa Amazônia Ocidental. |
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17. | | LEHMANN, J.; SILVA JUNIOR, J. P. da; RONDON, M.; CRAVO, M. da S.; GREENWOOD, J.; NEHLS, T.; STEINER, C.; GLASER, B. Slash-and-char: a feasible alternative for soil fertility management in the Central Amazon? In: WORLD CONGRESS OF SOIL SCIENCE, 17., 2002, Thailand. Proceedings... Bangkok: WCSS, 2002. p. 439. (WCSS. Symposium, 13). Paper n. 449. Biblioteca(s): Embrapa Amazônia Oriental. |
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18. | | TAMMEORG, P.; BASTOS, A. C.; JEFFERY, S.; REES, F.; KERN, J.; GRABER, E. R.; VENTURA, M.; AMARO, A.; BUDAI, A.; CORDOVIL, C. M. D. S.; DOMENE, X.; GARDI, C.; GASCÓ, G.; HORÁK, J.; KAMMANN, C. I.; KONDRLOVA, E.; LAIRD, D.; LOUREIRO, S.; MARTINS, M. A. S.; PANZACCHI, P.; PRASAD, M.; PRODANA, M.; PUGA, A. P.; RUYSSCHAERT, G.; SAS PASZT, L.; SILVA, F. C.; TEIXEIRA, W. G.; TONON, G.; DELLE VEDOVE, G.; ZAVALLONI, C.; GLASER, B.; VERHEIJEN, F. G. A. Biochars in soils: towards the required level of scientific understanding. Journal of Environmental Engineering and Landscape Management, v. 25, n. 2, p. 192-207, 2017. Biblioteca(s): Embrapa Solos. |
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19. | | LOMBARDO, U.; ARROYO-KALIN, M.; SCHMIDT, M.; HUISMAN, H.; LIMA, H. P.; MORAES, M. de P.; NEVES, E. G.; CLEMENT, C. R.; FONSECA, J. A. da; ALMEIDA, F. O. de; ALHO, C. F. B. V.; RAMSEY, C. B.; BROWN, G. G.; CAVALLINI, M. S.; COSTA, M. L. da; CUNHA, L.; ANJOS, L. H. C. dos; DENEVAN, W. M.; FAUSTO, C.; CAROMANO, C. F.; FONTANA, A.; FRANCHETTO, B.; GLASER, B.; HECKENBERGER, M. J.; HECHT, S.; HONORATO, V.; JAROSCH, K. A.; JUNQUEIRA, A. B.; KATER, T.; TAMANAHA, E. K.; KUYPER, T. W.; LEHMANN, J.; MADELLA, M.; MAEZUMI, S. Y.; CASCON, L. M.; MAYLE, F. E.; MCKEY, D.; MORAES, B.; MORCOTE-RÍOS, G.; BARBOSA, C. A. P.; MAGALHÃES, M. P.; PRESTES-CARNEIRO, G.; PUGLIESE, F.; PUPIM, F. N.; RACZKA, M. F.; PY-DANIEL, A. R.; ROCHA, B. C. da; RODRIGUES, L.; ROSTAIN, S.; MACEDO, R. S.; SHOCK, M. P.; SPRAFKE, T.; BASSI, F. S.; VALLE, R.; VIDAL-TORRADO, P.; VILLAGRÁN, X. S.; WATLING, J.; WEBER, S. L.; TEIXEIRA, W. G. Evidence confirms an anthropic origin of Amazonian Dark Earths. Nature Communications, v. 13, n. 3444, 2022. 6 p. Biblioteca(s): Embrapa Florestas; Embrapa Solos. |
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Registros recuperados : 19 | |
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Registro Completo
Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Soja. |
Data corrente: |
20/02/2014 |
Data da última atualização: |
05/04/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; BORÉM, A.; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L. |
Afiliação: |
UEL; CNPSo; NORMAN NEUMAIER, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; UFV; ANA CLAUDIA BARNECHE DE OLIVEIRA, CPACT; BEATRIZ MARTI EMYGDIO, CPACT; ALEXANDRE LIMA NEPOMUCENO, SRI. |
Título: |
Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 1, p. 860-871, 2014. |
DOI: |
DOI: 10.4238/2014.February.13.4 |
Idioma: |
Inglês |
Conteúdo: |
Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantification is an effective strategy for correcting random and systematic errors by using the expression level of reference gene(s) to normalize the expression level of the genes of interest. To identify soybean reference genes for use in studies of flooding stress, we compared 5 candidate reference genes (CRGs) with the NormFinder and GeNorm programs to select the best internal control. The expression stability of the CRGs was evaluated in root tissues from soybean plants subjected to hypoxic conditions. Elongation factor 1-beta and actin-11 were identified as the most appropriate genes for RT-qPCR normalization by both the NormFinder and GeNorm analyses. The expression profiles of the genes for alcohol dehydrogenase 1, sucrose synthase 4, and ascorbate peroxidase 2 were analyzed by comparing different normalizing combinations (including no normalization) of the selected reference genes. Here, we have identified potential genes for use as references for RT-qPCR normalization in experiments with soybean roots growing in O2-depleted environments, such as flooding-stressed plants. |
Palavras-Chave: |
Endogenous genes; Flooding; Housekeeping genes; Internal control genes. |
Thesaurus NAL: |
gene expression. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97888/1/nakayama.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97827/1/Ana-Claudia.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/112937/1/Reference-genes-soybean-..hypoxic-conditions-Incluido.pdf
|
Marc: |
LEADER 02358naa a2200301 a 4500 001 1980784 005 2022-04-05 008 2014 bl uuuu u00u1 u #d 024 7 $aDOI: 10.4238/2014.February.13.4$2DOI 100 1 $aNAKAYAMA, T. J. 245 $aReference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions.$h[electronic resource] 260 $c2014 520 $aQuantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantification is an effective strategy for correcting random and systematic errors by using the expression level of reference gene(s) to normalize the expression level of the genes of interest. To identify soybean reference genes for use in studies of flooding stress, we compared 5 candidate reference genes (CRGs) with the NormFinder and GeNorm programs to select the best internal control. The expression stability of the CRGs was evaluated in root tissues from soybean plants subjected to hypoxic conditions. Elongation factor 1-beta and actin-11 were identified as the most appropriate genes for RT-qPCR normalization by both the NormFinder and GeNorm analyses. The expression profiles of the genes for alcohol dehydrogenase 1, sucrose synthase 4, and ascorbate peroxidase 2 were analyzed by comparing different normalizing combinations (including no normalization) of the selected reference genes. Here, we have identified potential genes for use as references for RT-qPCR normalization in experiments with soybean roots growing in O2-depleted environments, such as flooding-stressed plants. 650 $agene expression 653 $aEndogenous genes 653 $aFlooding 653 $aHousekeeping genes 653 $aInternal control genes 700 1 $aRODRIGUES, F. A. 700 1 $aNEUMAIER, N. 700 1 $aMARCELINO-GUIMARÃES, F. C. 700 1 $aFARIAS, J. R. B. 700 1 $aOLIVEIRA, M. C. N. de 700 1 $aBORÉM, A. 700 1 $aOLIVEIRA, A. C. B. de 700 1 $aEMYGDIO, B. M. 700 1 $aNEPOMUCENO, A. L. 773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 13, n. 1, p. 860-871, 2014.
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