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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
21/12/2022 |
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Data da última atualização: |
21/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RECCHIA, K.; MACHADO, L. S.; BOTIGELLI, R. C.; PIERI, N. C. G.; BARBOSA, G.; CASTRO, R. V. G. de; MARQUES, M. G.; PESSÔA, L. V. de F.; FANTINATO NETO, P.; MEIRELLES, F. V.; SOUZA, A. F. de; MARTINS, S. M. M. K.; BRESSAN, F. F. |
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Afiliação: |
KAIANA RECCHIA, Universidade de São Paulo; LUCAS SIMÕES MACHADO, Universidade de São Paulo; RAMON CESAR BOTIGELLI, Universidade Estadual Paulista; NAIRA CAROLINE GODOY PIERI, Universidade de São Paulo; GABRIELA BARBOSA, Universidade de São Paulo; RAQUEL VASCONCELOS GUIMARÃES DE CASTRO, Universidade de São Paulo; MARIANA GROKE MARQUES, CNPSA; LAÍS VICARI DE FIGUEIREDO PESSÔA, Universidade de São Paulo; PAULO FANTINATO NETO, Universidade de São Paulo; FLÁVIO VIEIRA MEIRELLES, Universidade de São Paulo; ALINE FERNANDA DE SOUZA, Universidade de São Paulo; SIMONE MARIA MASSAMI KITAMURA MARTINS, Universidade de São Paulo; FABIANA FERNANDES BRESSAN, Universidade de São Paulo. |
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Título: |
In vitro induced pluripotency from urine-derived cells in porcine. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
World Journal of Stem Cells, v. 14, n. 3, p. 231-244, 2022. |
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DOI: |
https://doi.org/10.4252/wjsc.v14.i3.231 |
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Idioma: |
Inglês |
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Conteúdo: |
Methods: The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4 (OCT4), SRY-box2 (SOX2), Kruppel-like factor 4 (KLF4), and C-MYC, and cultured with basic fibroblast growth factor (bFGF) supplementation. To characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase (AP), OCT4, SOX2, NANOG, TRA1 81 and SSEA 1 detection. Endogenous transcripts related to the pluripotency (OCT4, SOX2 and NANOG) were analyzed via reverse transcription quantitative real-time polymerase chain reaction in different time points during the culture, and all three lineages formed embryoid bodies (EBs) when cultured in suspension without bFGF supplementation. Results: The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro reprogramming. Colonies of putative iPSCs were obtained only from UDCs transduced with the murine factors (mOSKM), but not from human factors (hOSKM). Three clonal lineages were isolated and further cultured for at least 28 passages, all the lineages were positive for AP detection, the OCT4, SOX2, NANOG markers, albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1, similar results were observed in the abundance of the endogenous transcripts related to pluripotent state. All the clonal lineages when cultured in suspension without bFGF were able to form EBs expressing ectoderm and mesoderm layers transcripts. Conclusion: For the first time UDCs were isolated in the swine model and reprogrammed into a pluripotent-like state, enabling new numerous applications in both human or veterinary regenerative medicine. MenosMethods: The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4 (OCT4), SRY-box2 (SOX2), Kruppel-like factor 4 (KLF4), and C-MYC, and cultured with basic fibroblast growth factor (bFGF) supplementation. To characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase (AP), OCT4, SOX2, NANOG, TRA1 81 and SSEA 1 detection. Endogenous transcripts related to the pluripotency (OCT4, SOX2 and NANOG) were analyzed via reverse transcription quantitative real-time polymerase chain reaction in different time points during the culture, and all three lineages formed embryoid bodies (EBs) when cultured in suspension without bFGF supplementation. Results: The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro reprogramming. Colonies of putative iPSCs were obtained only from UDCs transduced with the murine factors (mOSKM), but not from human factors (hOSKM). Three clonal lineages were isolated and further cultured for at least 28 passages, all the lineages were positive for AP detection, the OCT4, SOX2, NANOG markers, albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1, similar results were observed in the abundance of the endogenous transcripts related to pluripotent state. All the clonal lineages when cultured in suspension without bFGF were able to form EBs expressi... Mostrar Tudo |
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Palavras-Chave: |
Células-tronco; IPSC; Noninvasive; Pluripotência; Pluripotency; Reprogramming. |
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Thesagro: |
Suíno; Urina. |
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Thesaurus Nal: |
Induced pluripotent stem cells; Swine; Urine. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150134/1/10084.pdf
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Marc: |
LEADER 02880naa a2200409 a 4500
001 2150134
005 2022-12-21
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4252/wjsc.v14.i3.231$2DOI
100 1 $aRECCHIA, K.
245 $aIn vitro induced pluripotency from urine-derived cells in porcine.$h[electronic resource]
260 $c2022
520 $aMethods: The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4 (OCT4), SRY-box2 (SOX2), Kruppel-like factor 4 (KLF4), and C-MYC, and cultured with basic fibroblast growth factor (bFGF) supplementation. To characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase (AP), OCT4, SOX2, NANOG, TRA1 81 and SSEA 1 detection. Endogenous transcripts related to the pluripotency (OCT4, SOX2 and NANOG) were analyzed via reverse transcription quantitative real-time polymerase chain reaction in different time points during the culture, and all three lineages formed embryoid bodies (EBs) when cultured in suspension without bFGF supplementation. Results: The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro reprogramming. Colonies of putative iPSCs were obtained only from UDCs transduced with the murine factors (mOSKM), but not from human factors (hOSKM). Three clonal lineages were isolated and further cultured for at least 28 passages, all the lineages were positive for AP detection, the OCT4, SOX2, NANOG markers, albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1, similar results were observed in the abundance of the endogenous transcripts related to pluripotent state. All the clonal lineages when cultured in suspension without bFGF were able to form EBs expressing ectoderm and mesoderm layers transcripts. Conclusion: For the first time UDCs were isolated in the swine model and reprogrammed into a pluripotent-like state, enabling new numerous applications in both human or veterinary regenerative medicine.
650 $aInduced pluripotent stem cells
650 $aSwine
650 $aUrine
650 $aSuíno
650 $aUrina
653 $aCélulas-tronco
653 $aIPSC
653 $aNoninvasive
653 $aPluripotência
653 $aPluripotency
653 $aReprogramming
700 1 $aMACHADO, L. S.
700 1 $aBOTIGELLI, R. C.
700 1 $aPIERI, N. C. G.
700 1 $aBARBOSA, G.
700 1 $aCASTRO, R. V. G. de
700 1 $aMARQUES, M. G.
700 1 $aPESSÔA, L. V. de F.
700 1 $aFANTINATO NETO, P.
700 1 $aMEIRELLES, F. V.
700 1 $aSOUZA, A. F. de
700 1 $aMARTINS, S. M. M. K.
700 1 $aBRESSAN, F. F.
773 $tWorld Journal of Stem Cells$gv. 14, n. 3, p. 231-244, 2022.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
16/12/2013 |
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Data da última atualização: |
15/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
GARCIA, M.; VIGNA, B. B. Z.; SOUSA, A. C. B.; JUNGMANN, L.; CIDADE, F. W.; TOLEDO-SILVA, G.; FRANCISCO, P. M.; CHIARI, L.; CARVALHO, M. A.; KARIA, C. T.; FALEIRO, F. G.; GODOY, R.; DALL'AGNOL, M.; PAGLIARINI, S. S.; SOUZA, F. H. D. de; SOUZA-CHIES, T. T.; JANK, L.; RESENDE, R. M. S.; VALLE, C. B. do; ZUCCHI, M. I.; SOUZA, A. P. |
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Afiliação: |
MELISSA GARCIA, Universidade Estadual de Campinas; BIANCA BACCILI ZANOTTO VIGNA, CPPSE; ADNA C. B. SOUSA, Universidade Estadual de Campinas; LETICIA JUNGMANN, Universidade Estadual de Campinas; FERNANDA W. CIDADE, Universidade Estadual de Campinas; GUILHERME TOLEDO-SILVA, Universidade Estadual de Campinas; PATRÍCIA M. FRANCISCO, Universidade Estadual de Campinas; LUCIMARA CHIARI, Universidade Estadual de Campinas; MARCELO AYRES CARVALHO, CPAC; CLAUDIO TAKAO KARIA, CPAC; FABIO GELAPE FALEIRO, CPAC; RODOLFO GODOY, CPPSE; M. DALL''AGNOL, Universidade Federal do Rio Grande do Sul; SUELI S. PAGLIARINI, Universidade Estadual de Maringá; FRANCISCO HUMBERTO DUBBERN DE SOUZA, CPPSE; TATIANA T. SOUZA-CHIES, Universidade Federal do Rio Grande do Sul; LIANA JANK, CNPGC; ROSANGELA MARIA SIMEAO RESENDE, CNPGC; CACILDA BORGES DO VALLE, CNPGC; MARIA I. ZUCCHI, Agência Paulista de Tecnologia dos Agronegócios; ANETE P. SOUZA, Universidade Estadual de Campinas. |
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Título: |
Molecular genetic variability, population structure and mating system in tropical forages. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Tropical Grasslands. v. 1, p. 25-30, 2013. |
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Páginas: |
6p. |
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DOI: |
https://doi.org/10.17138/tgft(1)25-30 |
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Idioma: |
Inglês |
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Conteúdo: |
Microsatellite (SSR) markers were developed for the following tropical forage species, using accessions available from the plant genetic resources (PGR) collections held by EMBRAPA (Brazilian Agricultural Research Corporation): Brachiaria brizantha, B. humidicola, Panicum maximum, Paspalum spp., Stylosanthes capitata, S. guianensis, S. macrocephala, Calopogonium mucunoides and Centrosema spp. The markers were used to analyze population structure and genetic diversity, evolution and origin of the genetic variability in the center of origin, mating systems and genetic resources in EMBRAPA?s germplasm bank. The results shed light on the amount of genetic variation within and between populations, revealed the need in some cases for further plant collection to adequately represent the species in PGR collections, allowed us to assemble core collections (subsets of the total collections) that should contain most of the available diversity and (in the case of the legumes) showed the need to avoid unwanted outcrossing when regenerating conserved material. The data will allow plant breeders to better select accessions for hybrid production, discriminate between genotypes and use marker-assisted selection in breeding programs. Our results will also underpin the construction of genetic maps, mapping of genes of agronomic interest and numerous other studies on genetic variability, population structure, gene flow and reproductive systems for the tropical forage species studied in this work. MenosMicrosatellite (SSR) markers were developed for the following tropical forage species, using accessions available from the plant genetic resources (PGR) collections held by EMBRAPA (Brazilian Agricultural Research Corporation): Brachiaria brizantha, B. humidicola, Panicum maximum, Paspalum spp., Stylosanthes capitata, S. guianensis, S. macrocephala, Calopogonium mucunoides and Centrosema spp. The markers were used to analyze population structure and genetic diversity, evolution and origin of the genetic variability in the center of origin, mating systems and genetic resources in EMBRAPA?s germplasm bank. The results shed light on the amount of genetic variation within and between populations, revealed the need in some cases for further plant collection to adequately represent the species in PGR collections, allowed us to assemble core collections (subsets of the total collections) that should contain most of the available diversity and (in the case of the legumes) showed the need to avoid unwanted outcrossing when regenerating conserved material. The data will allow plant breeders to better select accessions for hybrid production, discriminate between genotypes and use marker-assisted selection in breeding programs. Our results will also underpin the construction of genetic maps, mapping of genes of agronomic interest and numerous other studies on genetic variability, population structure, gene flow and reproductive systems for the tropical forage species studied in this wor... Mostrar Tudo |
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Palavras-Chave: |
Microsatellites; Molecular markers; Pre breeding. |
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Thesaurus NAL: |
plant genetic resources. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94096/1/PROCI-2013.00194.pdf
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Marc: |
LEADER 02711naa a2200433 a 4500
001 1974023
005 2023-05-15
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.17138/tgft(1)25-30$2DOI
100 1 $aGARCIA, M.
245 $aMolecular genetic variability, population structure and mating system in tropical forages.$h[electronic resource]
260 $c2013
300 $a6p.
520 $aMicrosatellite (SSR) markers were developed for the following tropical forage species, using accessions available from the plant genetic resources (PGR) collections held by EMBRAPA (Brazilian Agricultural Research Corporation): Brachiaria brizantha, B. humidicola, Panicum maximum, Paspalum spp., Stylosanthes capitata, S. guianensis, S. macrocephala, Calopogonium mucunoides and Centrosema spp. The markers were used to analyze population structure and genetic diversity, evolution and origin of the genetic variability in the center of origin, mating systems and genetic resources in EMBRAPA?s germplasm bank. The results shed light on the amount of genetic variation within and between populations, revealed the need in some cases for further plant collection to adequately represent the species in PGR collections, allowed us to assemble core collections (subsets of the total collections) that should contain most of the available diversity and (in the case of the legumes) showed the need to avoid unwanted outcrossing when regenerating conserved material. The data will allow plant breeders to better select accessions for hybrid production, discriminate between genotypes and use marker-assisted selection in breeding programs. Our results will also underpin the construction of genetic maps, mapping of genes of agronomic interest and numerous other studies on genetic variability, population structure, gene flow and reproductive systems for the tropical forage species studied in this work.
650 $aplant genetic resources
653 $aMicrosatellites
653 $aMolecular markers
653 $aPre breeding
700 1 $aVIGNA, B. B. Z.
700 1 $aSOUSA, A. C. B.
700 1 $aJUNGMANN, L.
700 1 $aCIDADE, F. W.
700 1 $aTOLEDO-SILVA, G.
700 1 $aFRANCISCO, P. M.
700 1 $aCHIARI, L.
700 1 $aCARVALHO, M. A.
700 1 $aKARIA, C. T.
700 1 $aFALEIRO, F. G.
700 1 $aGODOY, R.
700 1 $aDALL'AGNOL, M.
700 1 $aPAGLIARINI, S. S.
700 1 $aSOUZA, F. H. D. de
700 1 $aSOUZA-CHIES, T. T.
700 1 $aJANK, L.
700 1 $aRESENDE, R. M. S.
700 1 $aVALLE, C. B. do
700 1 $aZUCCHI, M. I.
700 1 $aSOUZA, A. P.
773 $tTropical Grasslands.$gv. 1, p. 25-30, 2013.
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Embrapa Pecuária Sudeste (CPPSE) |
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