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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
18/09/1995 |
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Data da última atualização: |
12/04/2023 |
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Autoria: |
DORSEY, T. E.; McDONALD, P. W.; ROELS, O. A. |
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Afiliação: |
University of Texas Marine Institute, Port Aransas Laboratory, Port Aransas, Texas. |
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Título: |
A heated biuret-folin protein assay which gives equal absorbance with different proteins. |
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Ano de publicação: |
1977 |
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Fonte/Imprenta: |
Analytical Biochemistry, v.78, p.156-164, 1977. |
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Idioma: |
Inglês |
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Conteúdo: |
This protein assay requires heating the sample with biuret reagent for 100 min at 100grausC before addition of Folin. Different proteins give almost identical optical densities on a nitrogen basis with this procedure. The absorbance per microgram-atom of protein nitrogin is linear at or below 0.400. A convenient final concentration of protein in this assay is about 0.2 microg-atom of protein nitrogen per ml. Most of the absorbance is due to amino acid combinations other than tyrosine and tryptophan in the protein. The specificity appears similar to that of the biuret where few compounds of biological importance interfere significantly in the assay at normal cellular concentrations. |
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Palavras-Chave: |
Bioquimical Nitrogenio. |
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Thesagro: |
Proteína. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01199naa a2200169 a 4500
001 1647116
005 2023-04-12
008 1977 bl uuuu u00u1 u #d
100 1 $aDORSEY, T. E.
245 $aA heated biuret-folin protein assay which gives equal absorbance with different proteins.$h[electronic resource]
260 $c1977
520 $aThis protein assay requires heating the sample with biuret reagent for 100 min at 100grausC before addition of Folin. Different proteins give almost identical optical densities on a nitrogen basis with this procedure. The absorbance per microgram-atom of protein nitrogin is linear at or below 0.400. A convenient final concentration of protein in this assay is about 0.2 microg-atom of protein nitrogen per ml. Most of the absorbance is due to amino acid combinations other than tyrosine and tryptophan in the protein. The specificity appears similar to that of the biuret where few compounds of biological importance interfere significantly in the assay at normal cellular concentrations.
650 $aProteína
653 $aBioquimical Nitrogenio
700 1 $aMcDONALD, P. W.
700 1 $aROELS, O. A.
773 $tAnalytical Biochemistry$gv.78, p.156-164, 1977.
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| 1. |  | OLIVEIRA, V. A de; SANTOS, H. G. dos; RIOS, A. J. W.; JACOMINE, P. K. T.; PEREZ, D. V.; ARAÚJO, W. S. de; CALDERANO, S. B. Reunião de Correlação, Classificação e Aplicação de Levantamentos de Solos da Região Centro-Oeste: RCC - GO/MT. Rio de Janeiro: Embrapa Solos, 2004. 104 p. (Embrapa Solos. Boletim de pesquisa e desenvolvimento, 55).| Biblioteca(s): Embrapa Solos. |
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