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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/05/1995 |
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Data da última atualização: |
12/05/1995 |
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Autoria: |
LAVI, U.; AKKAYA, M.; BHAGWAT, A.; LAHAV, E.; CREGAN, P. B. |
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Afiliação: |
Instituto of horticulture; United states departament of agriculture. |
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Título: |
Methodology of generation and characteristics of simple sequence repeat DNA markers in avocado (Persea americana M.). |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Euphytica v.80, n.171-177, 1994. |
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ISSN: |
0014-2336 |
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Idioma: |
Inglês |
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Conteúdo: |
Generation of simple sequence repeat (SSR) DNA markers was based on the construction of genomic DNA library of avocado (Persea americana M.). The library was screened with the four dinucleotide probes (AG), (AT), (GC) and (CA). Positive clones were sequenced to validate the presence of simple sequence repeats (SSR) and to generate polymerase chain reaction (PCR) primers based on the sequence flanking the simple sequence repeat. Twenty six different pairs of primers which yield a PCR product in the initial screening were synthesized. The SSR A1E11 was found to have eleven alleles while A3F8 has eight alleles. The SSRs in avocado were found to be inherited in a Mendelian fashion. |
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Palavras-Chave: |
Avocado; Genectics; Microsatellites; PCR; SSR. |
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Thesagro: |
Persea Americana. |
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Thesaurus Nal: |
breeding. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01374naa a2200265 a 4500
001 1633167
005 1995-05-12
008 1994 bl uuuu u00u1 u #d
022 $a0014-2336
100 1 $aLAVI, U.
245 $aMethodology of generation and characteristics of simple sequence repeat DNA markers in avocado (Persea americana M.).
260 $c1994
520 $aGeneration of simple sequence repeat (SSR) DNA markers was based on the construction of genomic DNA library of avocado (Persea americana M.). The library was screened with the four dinucleotide probes (AG), (AT), (GC) and (CA). Positive clones were sequenced to validate the presence of simple sequence repeats (SSR) and to generate polymerase chain reaction (PCR) primers based on the sequence flanking the simple sequence repeat. Twenty six different pairs of primers which yield a PCR product in the initial screening were synthesized. The SSR A1E11 was found to have eleven alleles while A3F8 has eight alleles. The SSRs in avocado were found to be inherited in a Mendelian fashion.
650 $abreeding
650 $aPersea Americana
653 $aAvocado
653 $aGenectics
653 $aMicrosatellites
653 $aPCR
653 $aSSR
700 1 $aAKKAYA, M.
700 1 $aBHAGWAT, A.
700 1 $aLAHAV, E.
700 1 $aCREGAN, P. B.
773 $tEuphytica$gv.80, n.171-177, 1994.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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