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Registro Completo |
Biblioteca(s): |
Embrapa Soja; Embrapa Trigo; Embrapa Unidades Centrais. |
Data corrente: |
13/05/2016 |
Data da última atualização: |
17/08/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BORGES, C. W. C.; CARRÃO-PANIZZI, M. C.; MANDARINO, J. M. G.; SILVA, J. B. da; BENEDETTI, S.; IDA, E. I. |
Afiliação: |
BORGES, C. W. C., UEL; MERCEDES CONCORDIA CARRAO PANIZZI, CNPT; JOSE MARCOS GONTIJO MANDARINO, CNPSO; JOSEMEYRE BONIFÁCIO DA SILVA, UEL; SILVIA BENEDETTI, UEL; ELZA LOUKO IDA, UEL. |
Título: |
Contents and bioconversion of B-glycoside isoflavones to aglycones in the processing conditions of soybean tempeh. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF v. 51, n. 3, p. 271-279, mar. 2016. |
DOI: |
10.1590/S0100-204X2016000300009 |
Idioma: |
Inglês |
Conteúdo: |
ABSTRACT: The objective of this work was to evaluate the effect of the processing conditions of soybean tempeh on the contents of β‑glycoside isoflavones and on their bioconversion into aglycones. Different times of soaking (6, 12, and 18 hours), cooking (15, 30, and 45 minutes), and fermentation (18, 24, and 30 hours) with Rhizopus oligosporus at 37°C were evaluated for tempeh preparation. Grains from the cultivar 'BRS 267' were used, and the experiment was carried out according to a central composite design (23). The response functions comprised the contents of genistin, malonyldaidzin, malonylgenistin, daidzein, and genistein, quantified by ultraperformance liquid chromatography (UPLC). Soaking, cooking, and fermentation times change the content, profile, and distribution of the different forms of isoflavones in tempeh. The highest bioconversion of glycoside isoflavones into aglycones occurred in 6‑hour soaked soybean grains, whose cotyledons were cooked for 15 minutes and subjected to 18‑hour fermentation. RESUMO:O objetivo deste trabalho foi avaliar o efeito das condições de processamento do tempeh de soja sobre o conteúdo de isoflavonas ??glicosídeos e sobre sua bioconversão em agliconas. Diferentes tempos de maceração (6, 12 e 18 horas), cozimento (15, 30 e 45 minutos) e fermentação (18, 24 e 30 horas) com Rhizopus oligosporus a 37°C foram avaliados na preparação do tempeh. Foram utilizados grãos da cultivar 'BRS 267', e o experimento foi realizado de acordo com um delineamento composto central (23). As funções?respostas compreenderam o teor de genistina, malonildaidzina, malonilgenistina, daidzeína e genisteína, quantificadas por cromatografia líquida de ultraeficiência (CLUE). Os tempos de maceração, cozimento e fermentação alteraram o conteúdo, o perfil e a distribuição das diferentes formas de isoflavonas no tempeh. A maior bioconversão de ??glicosídeos em agliconas ocorreu em grãos de soja macerados por 6 horas, cujos cotilédones foram cozidos por 15 minutos e submetidos à fermentação por 18 horas. MenosABSTRACT: The objective of this work was to evaluate the effect of the processing conditions of soybean tempeh on the contents of β‑glycoside isoflavones and on their bioconversion into aglycones. Different times of soaking (6, 12, and 18 hours), cooking (15, 30, and 45 minutes), and fermentation (18, 24, and 30 hours) with Rhizopus oligosporus at 37°C were evaluated for tempeh preparation. Grains from the cultivar 'BRS 267' were used, and the experiment was carried out according to a central composite design (23). The response functions comprised the contents of genistin, malonyldaidzin, malonylgenistin, daidzein, and genistein, quantified by ultraperformance liquid chromatography (UPLC). Soaking, cooking, and fermentation times change the content, profile, and distribution of the different forms of isoflavones in tempeh. The highest bioconversion of glycoside isoflavones into aglycones occurred in 6‑hour soaked soybean grains, whose cotyledons were cooked for 15 minutes and subjected to 18‑hour fermentation. RESUMO:O objetivo deste trabalho foi avaliar o efeito das condições de processamento do tempeh de soja sobre o conteúdo de isoflavonas ??glicosídeos e sobre sua bioconversão em agliconas. Diferentes tempos de maceração (6, 12 e 18 horas), cozimento (15, 30 e 45 minutos) e fermentação (18, 24 e 30 horas) com Rhizopus oligosporus a 37°C foram avaliados na preparação do tempeh. Foram utilizados grãos da cultivar 'BRS 267', e o experimento foi realiz... Mostrar Tudo |
Palavras-Chave: |
Alimento funcional; Daidzeína; Fermented soybean; Genisteína; Rhizopus microsporus var oligosporus; Soja fermentada. |
Thesagro: |
Alimento fermentado; Fungo; Glycine Max; Nutrição humana; Rhizopus; Soja. |
Thesaurus Nal: |
Daidzein; Fermented foods; Functional foods; Genistein; Human nutrition; Soybeans. |
Categoria do assunto: |
-- Q Alimentos e Nutrição Humana |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/145844/1/ID43717-2016PABv5n3p271.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143013/1/Contents-and-bioconversion....pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143678/1/Contents-and-bioconversion.pdf
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Marc: |
LEADER 03293naa a2200409 a 4500 001 2049699 005 2021-08-17 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1590/S0100-204X2016000300009$2DOI 100 1 $aBORGES, C. W. C. 245 $aContents and bioconversion of B-glycoside isoflavones to aglycones in the processing conditions of soybean tempeh. 260 $c2016 520 $aABSTRACT: The objective of this work was to evaluate the effect of the processing conditions of soybean tempeh on the contents of β‑glycoside isoflavones and on their bioconversion into aglycones. Different times of soaking (6, 12, and 18 hours), cooking (15, 30, and 45 minutes), and fermentation (18, 24, and 30 hours) with Rhizopus oligosporus at 37°C were evaluated for tempeh preparation. Grains from the cultivar 'BRS 267' were used, and the experiment was carried out according to a central composite design (23). The response functions comprised the contents of genistin, malonyldaidzin, malonylgenistin, daidzein, and genistein, quantified by ultraperformance liquid chromatography (UPLC). Soaking, cooking, and fermentation times change the content, profile, and distribution of the different forms of isoflavones in tempeh. The highest bioconversion of glycoside isoflavones into aglycones occurred in 6‑hour soaked soybean grains, whose cotyledons were cooked for 15 minutes and subjected to 18‑hour fermentation. RESUMO:O objetivo deste trabalho foi avaliar o efeito das condições de processamento do tempeh de soja sobre o conteúdo de isoflavonas ??glicosídeos e sobre sua bioconversão em agliconas. Diferentes tempos de maceração (6, 12 e 18 horas), cozimento (15, 30 e 45 minutos) e fermentação (18, 24 e 30 horas) com Rhizopus oligosporus a 37°C foram avaliados na preparação do tempeh. Foram utilizados grãos da cultivar 'BRS 267', e o experimento foi realizado de acordo com um delineamento composto central (23). As funções?respostas compreenderam o teor de genistina, malonildaidzina, malonilgenistina, daidzeína e genisteína, quantificadas por cromatografia líquida de ultraeficiência (CLUE). Os tempos de maceração, cozimento e fermentação alteraram o conteúdo, o perfil e a distribuição das diferentes formas de isoflavonas no tempeh. A maior bioconversão de ??glicosídeos em agliconas ocorreu em grãos de soja macerados por 6 horas, cujos cotilédones foram cozidos por 15 minutos e submetidos à fermentação por 18 horas. 650 $aDaidzein 650 $aFermented foods 650 $aFunctional foods 650 $aGenistein 650 $aHuman nutrition 650 $aSoybeans 650 $aAlimento fermentado 650 $aFungo 650 $aGlycine Max 650 $aNutrição humana 650 $aRhizopus 650 $aSoja 653 $aAlimento funcional 653 $aDaidzeína 653 $aFermented soybean 653 $aGenisteína 653 $aRhizopus microsporus var oligosporus 653 $aSoja fermentada 700 1 $aCARRÃO-PANIZZI, M. C. 700 1 $aMANDARINO, J. M. G. 700 1 $aSILVA, J. B. da 700 1 $aBENEDETTI, S. 700 1 $aIDA, E. I. 773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 51, n. 3, p. 271-279, mar. 2016.
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Registro original: |
Embrapa Trigo (CNPT) |
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Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
18/06/2012 |
Data da última atualização: |
23/07/2012 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SOARES-CAVALCANTI, N. M.; BELARMINO, L. C.; KIDO, E. A.; PANDOLFI, V.; MARCELINO-GUIMARÃES, F. C.; RODRIGUES, F. A.; PEREIRA, G. A. G.; BENKO-ISEPPON, A. M. |
Afiliação: |
NINA M. SOARES-CAVALCANTI, UFPE; LUÍS C. BELARMINO, UFPE; EDERSON A. KIDO, UFPE; VALESCA PANDOLFI, UFPE; FRANCISMAR CORREA MARCELINO, CNPSO; FABIANA A. RODRIGUES, CNPSo; GONÇALO A. G. PEREIRA, UNICAMP; ANA M. BENKO-ISEPPON, UFPE. |
Título: |
Overall picture of expressed Heat Shock Factors in Glycine max, Lotus japonicus and Medicago truncatula. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 247-259, May 2012. |
Idioma: |
Inglês |
Conteúdo: |
Heat shock (HS) leads to the activation of molecular mechanisms, known as HS-response, that prevent damage and enhance survival under stress. Plants have a flexible and specialized network of Heat Shock Factors (HSFs), which are transcription factors that induce the expression of heat shock proteins. The present work aimed to identify and characterize the Glycine max HSF repertory in the Soybean Genome Project (GENOSOJA platform), comparing them with other legumes (Medicago truncatula and Lotus japonicus) in view of current knowledge of Arabidopsis thaliana. The HSF characterization in leguminous plants led to the identification of 25, 19 and 21 candidate ESTs in soybean, Lotus and Medicago, respectively. A search in the SuperSAGE libraries revealed 68 tags distributed in seven HSF gene types. From the total number of obtained tags, more than 70% were related to root tissues (water deficit stress libraries vs. controls), indicating their role in abiotic stress responses, since the root is the first tissue to sense and respond to abiotic stress. Moreover, as heat stress is related to the pressure of dryness, a higher HSF expression was expected at the water deficit libraries. On the other hand, expressive HSF candidates were obtained from the library inoculated with Asian Soybean Rust, inferring crosstalk among genes associated with abiotic and biotic stresses. Evolutionary relationships among sequences were consistent with different HSF classes and subclasses. Expression profiling indicated that regulation of specific genes is associated with the stage of plant development and also with stimuli from other abiotic stresses pointing to the maintenance of HSF expression at a basal level in soybean, favoring its activation under heat-stress conditions. MenosHeat shock (HS) leads to the activation of molecular mechanisms, known as HS-response, that prevent damage and enhance survival under stress. Plants have a flexible and specialized network of Heat Shock Factors (HSFs), which are transcription factors that induce the expression of heat shock proteins. The present work aimed to identify and characterize the Glycine max HSF repertory in the Soybean Genome Project (GENOSOJA platform), comparing them with other legumes (Medicago truncatula and Lotus japonicus) in view of current knowledge of Arabidopsis thaliana. The HSF characterization in leguminous plants led to the identification of 25, 19 and 21 candidate ESTs in soybean, Lotus and Medicago, respectively. A search in the SuperSAGE libraries revealed 68 tags distributed in seven HSF gene types. From the total number of obtained tags, more than 70% were related to root tissues (water deficit stress libraries vs. controls), indicating their role in abiotic stress responses, since the root is the first tissue to sense and respond to abiotic stress. Moreover, as heat stress is related to the pressure of dryness, a higher HSF expression was expected at the water deficit libraries. On the other hand, expressive HSF candidates were obtained from the library inoculated with Asian Soybean Rust, inferring crosstalk among genes associated with abiotic and biotic stresses. Evolutionary relationships among sequences were consistent with different HSF classes and subclasses. Expression pro... Mostrar Tudo |
Palavras-Chave: |
Bioinformática. |
Thesagro: |
Gene; Genoma; Soja. |
Thesaurus NAL: |
Abiotic stress; Bioinformatics; Genes; Genome; Soybeans; Transcription factors. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/61075/1/gmb.overall.v35n1s.247-259.2012.pdf
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Marc: |
LEADER 02713naa a2200325 a 4500 001 1926601 005 2012-07-23 008 2012 bl uuuu u00u1 u #d 100 1 $aSOARES-CAVALCANTI, N. M. 245 $aOverall picture of expressed Heat Shock Factors in Glycine max, Lotus japonicus and Medicago truncatula. 260 $c2012 520 $aHeat shock (HS) leads to the activation of molecular mechanisms, known as HS-response, that prevent damage and enhance survival under stress. Plants have a flexible and specialized network of Heat Shock Factors (HSFs), which are transcription factors that induce the expression of heat shock proteins. The present work aimed to identify and characterize the Glycine max HSF repertory in the Soybean Genome Project (GENOSOJA platform), comparing them with other legumes (Medicago truncatula and Lotus japonicus) in view of current knowledge of Arabidopsis thaliana. The HSF characterization in leguminous plants led to the identification of 25, 19 and 21 candidate ESTs in soybean, Lotus and Medicago, respectively. A search in the SuperSAGE libraries revealed 68 tags distributed in seven HSF gene types. From the total number of obtained tags, more than 70% were related to root tissues (water deficit stress libraries vs. controls), indicating their role in abiotic stress responses, since the root is the first tissue to sense and respond to abiotic stress. Moreover, as heat stress is related to the pressure of dryness, a higher HSF expression was expected at the water deficit libraries. On the other hand, expressive HSF candidates were obtained from the library inoculated with Asian Soybean Rust, inferring crosstalk among genes associated with abiotic and biotic stresses. Evolutionary relationships among sequences were consistent with different HSF classes and subclasses. Expression profiling indicated that regulation of specific genes is associated with the stage of plant development and also with stimuli from other abiotic stresses pointing to the maintenance of HSF expression at a basal level in soybean, favoring its activation under heat-stress conditions. 650 $aAbiotic stress 650 $aBioinformatics 650 $aGenes 650 $aGenome 650 $aSoybeans 650 $aTranscription factors 650 $aGene 650 $aGenoma 650 $aSoja 653 $aBioinformática 700 1 $aBELARMINO, L. C. 700 1 $aKIDO, E. A. 700 1 $aPANDOLFI, V. 700 1 $aMARCELINO-GUIMARÃES, F. C. 700 1 $aRODRIGUES, F. A. 700 1 $aPEREIRA, G. A. G. 700 1 $aBENKO-ISEPPON, A. M. 773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 35, n. 1, suppl., p. 247-259, May 2012.
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