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Registro Completo |
Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
Data corrente: |
03/04/2018 |
Data da última atualização: |
04/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PEIXOTO, F. M.; GOUVÊA, A. C. M. S.; SANTIAGO, M. C. P. de A.; MARTINS, Z. E. de S. V.; BORGUINI, R. G.; GODOY, R. L. de O. |
Afiliação: |
F. M. Peixoto, UFRRJ; A. C. M. Senna Gouvêa, UFRRJ; MANUELA CRISTINA P DE A SANTIAGO, CTAA; Z .E. de Sá Velosos Martins, Universidade do Porto; RENATA GALHARDO BORGUINI, CTAA; RONOEL LUIZ DE OLIVEIRA GODOY, CTAA. |
Título: |
Characterization and bioaccessibility of anthocyanins from blueberry (Vaccinium corymbosum L.) after simulated gastrointestinal digestion: A positive effect on malvidin derivatives. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Fruits, v. 73, n. 2, p. 88-96, mar./apr. 2018. |
ISSN: |
0248-1294 print, 1625-967X online |
DOI: |
10.17660/th2018/73.2.2 |
Idioma: |
Inglês |
Conteúdo: |
Recent studies have reported many preventive and therapeutic properties against cardiovascular disease and cancer, associated to the consumption of polyphenols, including anthocyanins. Anthocyanin bioavailability is a major issue regarding their biological effects, because the results are still controversial and divergent. Bioaccessibility and absorption studies represent the first steps in predicting bioavailability of the bioactive compounds in humans. The objectives of the current research were to perform an in vitro gastro-intestinal digestion model by mimicking oral, gastric and intestinal physiological conditions, in order to investigate the bioaccessibility of blueberry anthocyanins. Materials and methods – Identification and quantification of anthocyanins were carried out by HPLC and ESI-MS/MS detector. Results and discussion – Total anthocyanin content (125.27 ± 19.80 mg 100 g-1) was greatly decreased after digestion (6.50 ± 1.03 mg 100 g-1), which represented 5.20 ± 0.82% of bioaccessibility. All delphinidin derivatives were unstable, not detected after digestion, and had the lowest bioaccessible forms. Otherwise, malvidin derivatives, including the acetyl form, were stable and displayed the highest bioaccessibility percentage. More -OCH3 groups seems to increase the bioaccessibility, because higher structure size molecules can promote steric hindrance and impact on anthocyanin low degradation or conversion to non-detectable forms. Conclusion – These results should contribute to answer some questions concerning anthocyanin stability during gastro-intestinal path. MenosRecent studies have reported many preventive and therapeutic properties against cardiovascular disease and cancer, associated to the consumption of polyphenols, including anthocyanins. Anthocyanin bioavailability is a major issue regarding their biological effects, because the results are still controversial and divergent. Bioaccessibility and absorption studies represent the first steps in predicting bioavailability of the bioactive compounds in humans. The objectives of the current research were to perform an in vitro gastro-intestinal digestion model by mimicking oral, gastric and intestinal physiological conditions, in order to investigate the bioaccessibility of blueberry anthocyanins. Materials and methods – Identification and quantification of anthocyanins were carried out by HPLC and ESI-MS/MS detector. Results and discussion – Total anthocyanin content (125.27 ± 19.80 mg 100 g-1) was greatly decreased after digestion (6.50 ± 1.03 mg 100 g-1), which represented 5.20 ± 0.82% of bioaccessibility. All delphinidin derivatives were unstable, not detected after digestion, and had the lowest bioaccessible forms. Otherwise, malvidin derivatives, including the acetyl form, were stable and displayed the highest bioaccessibility percentage. More -OCH3 groups seems to increase the bioaccessibility, because higher structure size molecules can promote steric hindrance and impact on anthocyanin low degradation or conversion to non-detectable forms. Conclusion – These results should... Mostrar Tudo |
Palavras-Chave: |
Blueberry; Nutritional value; Phenolics. |
Thesagro: |
Vaccinium Corymbosum. |
Thesaurus Nal: |
antioxidant activity; in vitro digestion. |
Categoria do assunto: |
Q Alimentos e Nutrição Humana |
Marc: |
LEADER 02557naa a2200277 a 4500 001 2090182 005 2018-05-04 008 2018 bl uuuu u00u1 u #d 022 $a0248-1294 print, 1625-967X online 024 7 $a10.17660/th2018/73.2.2$2DOI 100 1 $aPEIXOTO, F. M. 245 $aCharacterization and bioaccessibility of anthocyanins from blueberry (Vaccinium corymbosum L.) after simulated gastrointestinal digestion$bA positive effect on malvidin derivatives.$h[electronic resource] 260 $c2018 520 $aRecent studies have reported many preventive and therapeutic properties against cardiovascular disease and cancer, associated to the consumption of polyphenols, including anthocyanins. Anthocyanin bioavailability is a major issue regarding their biological effects, because the results are still controversial and divergent. Bioaccessibility and absorption studies represent the first steps in predicting bioavailability of the bioactive compounds in humans. The objectives of the current research were to perform an in vitro gastro-intestinal digestion model by mimicking oral, gastric and intestinal physiological conditions, in order to investigate the bioaccessibility of blueberry anthocyanins. Materials and methods – Identification and quantification of anthocyanins were carried out by HPLC and ESI-MS/MS detector. Results and discussion – Total anthocyanin content (125.27 ± 19.80 mg 100 g-1) was greatly decreased after digestion (6.50 ± 1.03 mg 100 g-1), which represented 5.20 ± 0.82% of bioaccessibility. All delphinidin derivatives were unstable, not detected after digestion, and had the lowest bioaccessible forms. Otherwise, malvidin derivatives, including the acetyl form, were stable and displayed the highest bioaccessibility percentage. More -OCH3 groups seems to increase the bioaccessibility, because higher structure size molecules can promote steric hindrance and impact on anthocyanin low degradation or conversion to non-detectable forms. Conclusion – These results should contribute to answer some questions concerning anthocyanin stability during gastro-intestinal path. 650 $aantioxidant activity 650 $ain vitro digestion 650 $aVaccinium Corymbosum 653 $aBlueberry 653 $aNutritional value 653 $aPhenolics 700 1 $aGOUVÊA, A. C. M. S. 700 1 $aSANTIAGO, M. C. P. de A. 700 1 $aMARTINS, Z. E. de S. V. 700 1 $aBORGUINI, R. G. 700 1 $aGODOY, R. L. de O. 773 $tFruits$gv. 73, n. 2, p. 88-96, mar./apr. 2018.
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Embrapa Agroindústria de Alimentos (CTAA) |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
22/08/2011 |
Data da última atualização: |
27/08/2011 |
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
Autoria: |
SANTOS, S. N.; CASTANHA, R. F.; HABER, L. L.; MARQUES, M. O. M.; SCRAMIN, S.; MELO, I. S. de. |
Afiliação: |
SUIKINAI NOBRE SANTOS, ESALQ-USP; RODRIGO FERNANDES CASTANHA, CNPMA; LENITA LIMA HABER, IAC; MÁRCIA ORTIZ MAYO MARQUES, IAC; SHIRLEI SCRAMIN, CNPMA; ITAMAR SOARES DE MELO, CNPMA. |
Título: |
Determinação quantitativa da atividade antioxidante de extratos brutos de microrganismos pelo método de captura de radical livre DPPH. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Jaguariúna: Embrapa Meio Ambiente, 2011. |
Páginas: |
5 p. |
Série: |
(Embrapa Meio Ambiente. Comunicado Técnico, 50). |
Idioma: |
Português |
Conteúdo: |
O escopo deste trabalho é a disponibilização da metodologia científica utilizada na determinação quantitativa da atividade antioxidante de extratos livres DPPH, baseadas nas adaptações realizadas no Laboratório de Microbiologia Ambiental em parceria com o Laboratório de Produtos Naturais, ambos da Embrapa Meio Ambiente. |
Palavras-Chave: |
Radical livre. |
Thesagro: |
Análise bacteriológica; Análise de laboratório; Análise microbiológica; Método; Microrganismo. |
Thesaurus NAL: |
Free radicals; Laboratory techniques. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/40220/1/comunicado-50.pdf
|
Marc: |
LEADER 01250nam a2200289 a 4500 001 1898412 005 2011-08-27 008 2011 bl uuuu u0uu1 u #d 100 1 $aSANTOS, S. N. 245 $aDeterminação quantitativa da atividade antioxidante de extratos brutos de microrganismos pelo método de captura de radical livre DPPH.$h[electronic resource] 260 $aJaguariúna: Embrapa Meio Ambiente$c2011 300 $a5 p. 490 $a(Embrapa Meio Ambiente. Comunicado Técnico, 50). 520 $aO escopo deste trabalho é a disponibilização da metodologia científica utilizada na determinação quantitativa da atividade antioxidante de extratos livres DPPH, baseadas nas adaptações realizadas no Laboratório de Microbiologia Ambiental em parceria com o Laboratório de Produtos Naturais, ambos da Embrapa Meio Ambiente. 650 $aFree radicals 650 $aLaboratory techniques 650 $aAnálise bacteriológica 650 $aAnálise de laboratório 650 $aAnálise microbiológica 650 $aMétodo 650 $aMicrorganismo 653 $aRadical livre 700 1 $aCASTANHA, R. F. 700 1 $aHABER, L. L. 700 1 $aMARQUES, M. O. M. 700 1 $aSCRAMIN, S. 700 1 $aMELO, I. S. de
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