| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
29/12/2014 |
|
Data da última atualização: |
07/04/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BENCKE-MALATO, M.; CABREIRA, C.; WIEBKE-STROHM, B.; BÜCKER-NETO, L.; MANCINI, E.; OSORIO, M. B.; HOMRICH, M. S.; TURCHETTO-ZOLET, A. C.; CARVALHO, M. C. C. G. de; STOLF, R.; WEBER, R. L. M.; WESTERGAARD, G.; CASTAGNARO, A. P.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C.; MARGIS-PINHEIRO, M.; BODANESE-ZANETTINI, M. H. |
|
Afiliação: |
MARTA BENCKE-MALATO, UFRGS; CAROLINE CABREIRA, UFRGS; BEATRIZ WIEBKE-STROHM, UFRGS; LAURO BÜCKER-NETO, UFRGS; ESTEFANIA MANCINI, Instituto de Agrobiotecnologia Rosario SA; MARINA B. OSORIO, UFRGS; MILENA S. HOMRICH, UFRGS; ANDREIA CARINA TURCHETTO-ZOLET, UFRGS; MAYRA C. C. G. DE CARVALHO, CNPSO - estagiária; RENATA STOLF, CNPSO - estagiària; RICARDO L. M. WEBER, UFRGS; GASTÓN WESTERGAARD, Instituto de Agrobiotecnologia Rosario SA; ATÍLIO P. CASTAGNARO, Estación Experimental Agroindustrial Obispo Colombres (EEAOC); RICARDO VILELA ABDELNOOR, CNPSO; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO; MÁRCIA MARGIS-PINHEIRO, UFRGS; MARIA HELENA BODANESE-ZANETTINI, UFRGS. |
|
Título: |
Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
BMC Plant Biology, v. 14, n. 1, article 236, Sept. 2014. |
|
Páginas: |
18 p. |
|
ISSN: |
1471-2229 |
|
DOI: |
10.1186/s12870-014-0236-0 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified. Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants. Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants. MenosBackground: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified. Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than ... Mostrar Tudo |
|
Thesagro: |
Soja. |
|
Thesaurus Nal: |
Soybeans. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114583/1/Genome-wide-annotation-of-the-soybean-WRKY-family-and-functional-characterization-of-genes-involved-in-response-to-Phakopsora-pachyrhizi-infection.pdf
|
|
Marc: |
LEADER 02998naa a2200373 a 4500
001 2003866
005 2022-04-07
008 2014 bl uuuu u00u1 u #d
022 $a1471-2229
024 7 $a10.1186/s12870-014-0236-0$2DOI
100 1 $aBENCKE-MALATO, M.
245 $aGenome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection.$h[electronic resource]
260 $c2014
300 $a18 p.
520 $aBackground: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified. Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants. Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.
650 $aSoybeans
650 $aSoja
700 1 $aCABREIRA, C.
700 1 $aWIEBKE-STROHM, B.
700 1 $aBÜCKER-NETO, L.
700 1 $aMANCINI, E.
700 1 $aOSORIO, M. B.
700 1 $aHOMRICH, M. S.
700 1 $aTURCHETTO-ZOLET, A. C.
700 1 $aCARVALHO, M. C. C. G. de
700 1 $aSTOLF, R.
700 1 $aWEBER, R. L. M.
700 1 $aWESTERGAARD, G.
700 1 $aCASTAGNARO, A. P.
700 1 $aABDELNOOR, R. V.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aBODANESE-ZANETTINI, M. H.
773 $tBMC Plant Biology$gv. 14, n. 1, article 236, Sept. 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
19/08/2005 |
|
Data da última atualização: |
07/04/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
MELO, P. G. S.; MELO, L. C.; SOARES, A. A.; LIMA, L. M. de; REIS, M. de S.; JULIATTI, F. C.; CORNÉLIO, V. M. O. |
|
Afiliação: |
PATRÍCIA GUIMARÃES SANTOS MELO, UFG; LEONARDO CUNHA MELO, CNPAF; ANTÔNIO ALVES SOARES, UFLA; LUCIANA MARIA DE LIMA, UFLA; MOIZÉS DE SOUSA REIS, EPAMIG; FERNANDO CEZAR JULIATTI, UFU; VANDA MARIA OLIVEIRA CORNÉLIO, EPAMIG. |
|
Título: |
Study of the interaction genotypes x environments in the selection process of upland rice. |
|
Ano de publicação: |
2005 |
|
Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, v. 5, n. 1, p. 38-46, Mar. 2005. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The objectives of this study were the investigation of the interactions between upland rice lines/cultivars x planting system, years and locations. The experiments were conducted in the agricultural years of 2001/02 and 2002/03 in Lavras, Uberlandia and Patrocinio in the state of Minas Gerais (MG), Brazil, in two planting systems: conventional and no-tillage. The treatments were 20 lines/cultivars and the evaluated traits were: plant height, flowering date, grain yield and disease incidence. The absence genotype x system interaction of grain yield indicated that cultivars recommended for sowing traditional can be utilized in the no-tillage system. The interactions genotypes x locations and genotypes x years were significant. The Cruz methodology indicated that cultivar Caiapo presented adaptation in unfavourable environments and the line CNAs 8983 showed response in the environment improvement. The Annicchiarico methodology showed that Guarani and CNAs 8989 were the most stable. |
|
Palavras-Chave: |
Estabilidade; No-tillage sowing; Stability. |
|
Thesagro: |
Arroz; Melhoramento; Oryza Sativa; Plantio Direto. |
|
Thesaurus NAL: |
breeding; rice. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/24017/1/bd6b8337-3e42-29fd.pdf
|
|
Marc: |
LEADER 01838naa a2200301 a 4500
001 1213579
005 2022-04-07
008 2005 bl uuuu u00u1 u #d
100 1 $aMELO, P. G. S.
245 $aStudy of the interaction genotypes x environments in the selection process of upland rice.$h[electronic resource]
260 $c2005
520 $aThe objectives of this study were the investigation of the interactions between upland rice lines/cultivars x planting system, years and locations. The experiments were conducted in the agricultural years of 2001/02 and 2002/03 in Lavras, Uberlandia and Patrocinio in the state of Minas Gerais (MG), Brazil, in two planting systems: conventional and no-tillage. The treatments were 20 lines/cultivars and the evaluated traits were: plant height, flowering date, grain yield and disease incidence. The absence genotype x system interaction of grain yield indicated that cultivars recommended for sowing traditional can be utilized in the no-tillage system. The interactions genotypes x locations and genotypes x years were significant. The Cruz methodology indicated that cultivar Caiapo presented adaptation in unfavourable environments and the line CNAs 8983 showed response in the environment improvement. The Annicchiarico methodology showed that Guarani and CNAs 8989 were the most stable.
650 $abreeding
650 $arice
650 $aArroz
650 $aMelhoramento
650 $aOryza Sativa
650 $aPlantio Direto
653 $aEstabilidade
653 $aNo-tillage sowing
653 $aStability
700 1 $aMELO, L. C.
700 1 $aSOARES, A. A.
700 1 $aLIMA, L. M. de
700 1 $aREIS, M. de S.
700 1 $aJULIATTI, F. C.
700 1 $aCORNÉLIO, V. M. O.
773 $tCrop Breeding and Applied Biotechnology$gv. 5, n. 1, p. 38-46, Mar. 2005.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
