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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
12/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, M. R.; ROCHA, A. S.; COSTA, R. R.; ALENCAR, A. P.; OLIVEIRA, V. M. de; FONSECA JÚNIOR, A. A.; SALES, M. L.; ISSA, M. O.; SOARES FILHO, P. M.; PEREIRA, O. T. V.; SANTOS, E. C.; MENDES, R. S.; FERREIRA, A. M. de J.; MOTA, P. M. P. C.; SUFFYS, P. N.; GUIMARAES, M. D. C. |
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Afiliação: |
MARCIO ROBERTO SILVA, CNPGL; Adalgiza da Silva Rocha, FIOCRUZ RJ; Ronaldo Rodrigues da Costa, FHEMIG Juiz de Fora; Andrea Padilha de Alencar, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; VANIA MARIA DE OLIVEIRA, CNPGL; Antônio Augusto Fonseca Júnior, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Mariana Lázaro Sales, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Marina de Azevedo Issa, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Paulo Martins Soares Filho, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Omara Tereza Vianello Pereira, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Eduardo Calazans dos Santos, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Rejane Silva Mendes, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Ângela Maria de Jesus Ferreira, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Pedro Moacyr Pinto Coelho Mota, Laboratório Nacional Agropecuário, MAPA, Pedro Leopoldo, MG; Philip Noel Suffys, FIOCRUZ RJ; Mark Drew Crosland Guimarães, UFMG. |
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Título: |
Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Memórias do Instituto Oswaldo Cruz, v. 108, n. 3, p. 321-327, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB) patients living in an urban area in Brazil were characterised from 2008-2010 using phenotypic and molecular speciation methods (pncA gene and oxyR pseudogene analysis). Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ) and Stonebrink (SB)-containing media and presented phenotypic and molecular profiles of Mycobacterium tuberculosis, whereas 12 had molecular profiles of M. tuberculosis based on the DNA analysis of formalin-fixed paraffin wax-embedded tissue samples (paraffin blocks). One patient produced two sputum isolates, the first of which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, and the second of which only grew on SB media and presented phenotypic profiles of Mycobacterium bovis. One patient provided a bronchial lavage isolate, which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, but had molecular profiles of M. bovis from paraffin block DNA analysis, and one sample had molecular profiles of M. tuberculosis and M. bovis identified from two distinct paraffin blocks. Moreover, we found a low prevalence (1.6%) of M. bovis among these isolates, which suggests that local health service procedures likely underestimate its real frequency and that it deserves more attention from public health officials. |
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Palavras-Chave: |
Zoonotic tuberculosis. |
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Thesagro: |
Mycobacterium Bovis. |
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Thesaurus Nal: |
Mycobacterium tuberculosis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97214/1/Artigo-Marcio-Tuberculosis-0074-0276-mioc-108-03-00321.pdf
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Marc: |
LEADER 02490naa a2200337 a 4500
001 1979618
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
100 1 $aSILVA, M. R.
245 $aTuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil.$h[electronic resource]
260 $c2013
520 $aIn this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB) patients living in an urban area in Brazil were characterised from 2008-2010 using phenotypic and molecular speciation methods (pncA gene and oxyR pseudogene analysis). Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ) and Stonebrink (SB)-containing media and presented phenotypic and molecular profiles of Mycobacterium tuberculosis, whereas 12 had molecular profiles of M. tuberculosis based on the DNA analysis of formalin-fixed paraffin wax-embedded tissue samples (paraffin blocks). One patient produced two sputum isolates, the first of which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, and the second of which only grew on SB media and presented phenotypic profiles of Mycobacterium bovis. One patient provided a bronchial lavage isolate, which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, but had molecular profiles of M. bovis from paraffin block DNA analysis, and one sample had molecular profiles of M. tuberculosis and M. bovis identified from two distinct paraffin blocks. Moreover, we found a low prevalence (1.6%) of M. bovis among these isolates, which suggests that local health service procedures likely underestimate its real frequency and that it deserves more attention from public health officials.
650 $aMycobacterium tuberculosis
650 $aMycobacterium Bovis
653 $aZoonotic tuberculosis
700 1 $aROCHA, A. S.
700 1 $aCOSTA, R. R.
700 1 $aALENCAR, A. P.
700 1 $aOLIVEIRA, V. M. de
700 1 $aFONSECA JÚNIOR, A. A.
700 1 $aSALES, M. L.
700 1 $aISSA, M. O.
700 1 $aSOARES FILHO, P. M.
700 1 $aPEREIRA, O. T. V.
700 1 $aSANTOS, E. C.
700 1 $aMENDES, R. S.
700 1 $aFERREIRA, A. M. de J.
700 1 $aMOTA, P. M. P. C.
700 1 $aSUFFYS, P. N.
700 1 $aGUIMARAES, M. D. C.
773 $tMemórias do Instituto Oswaldo Cruz$gv. 108, n. 3, p. 321-327, 2013.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/12/2022 |
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Data da última atualização: |
12/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CARVALHO, M. de J. da S. de; SOUZA, A. da S.; EMANUELA BARBOSA SANTOS; SOARES FILHO, W. dos S.; LEDO, C. A. da S.; COSTA, E. M. R.; SOUZA, F. V. D. |
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Afiliação: |
MARIANE DE JESUS DA SILVA DE CARVALHO, UNIVERSIDADE FEDERAL DO RECÔNCAVO DA BAHIA; ANTONIO DA SILVA SOUZA, CNPMF; EMANUELA BARBOSA SANTOS, UNIVERSIDADE FEDERAL DO CEARÁ; WALTER DOS SANTOS SOARES FILHO, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; EVA MARIA RODRIGUES COSTA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
In vitro conservation of 'Florida Rough' lemon plants. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Ciência Rural, Santa Maria, v.52:12, e20210276, 2022. |
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ISSN: |
1678-4596 |
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DOI: |
https://doi.org/10.1590/0103-8478cr20210276 |
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Idioma: |
Inglês |
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Conteúdo: |
The establishment of minimum growth conditions is essential for in vitro germplasm conservation. Changes to the basic medium and carbon source concentrations are important factors for reducing plant growth in vitro. This study adjusted a protocol for the in vitro conservation of ?Florida Rough? lemon plants. Microcuttings (approximately 1 cm) from plants that were previously cultivated in vitro were inoculated into test tubes with 10 mL of woody plant medium (WPM) at different concentrations (1/1, 1/2 and 1/4) and supplemented with 0, 12.5, 25 and 50 g.L-1 of sucrose, solidified with 7 g.L-1 agar and adjusted to pH 5.8. The experiment was completely randomized in a 3 x 4 factorial design with 15 replications and was maintained under controlled conditions for 360 days. After this period the plant height in cm (PH), the plant dry mass in g (PDM) and the 21 numbers of green leaves (NGL), senescent leaves (NSL) and microcuttings (NM) were evaluated. The variables that best explained the observed behavior of the ?Florida Rough? lemon plants were NGL and PH, with values of 61.63 and 35.08%, respectively. The original concentration of the WPM with the addition of 25 g. L-1 of sucrose yielded the best ?Florida Rough? lemon plant growth reduction in vitro while maintaining the physiologically health of the plants. |
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Thesagro: |
Cultura In Vitro; Limão. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1149407/1/In-vitro-conservation.pdf
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Marc: |
LEADER 02052naa a2200241 a 4500
001 2149407
005 2022-12-12
008 2022 bl uuuu u00u1 u #d
022 $a1678-4596
024 7 $ahttps://doi.org/10.1590/0103-8478cr20210276$2DOI
100 1 $aCARVALHO, M. de J. da S. de
245 $aIn vitro conservation of 'Florida Rough' lemon plants.$h[electronic resource]
260 $c2022
520 $aThe establishment of minimum growth conditions is essential for in vitro germplasm conservation. Changes to the basic medium and carbon source concentrations are important factors for reducing plant growth in vitro. This study adjusted a protocol for the in vitro conservation of ?Florida Rough? lemon plants. Microcuttings (approximately 1 cm) from plants that were previously cultivated in vitro were inoculated into test tubes with 10 mL of woody plant medium (WPM) at different concentrations (1/1, 1/2 and 1/4) and supplemented with 0, 12.5, 25 and 50 g.L-1 of sucrose, solidified with 7 g.L-1 agar and adjusted to pH 5.8. The experiment was completely randomized in a 3 x 4 factorial design with 15 replications and was maintained under controlled conditions for 360 days. After this period the plant height in cm (PH), the plant dry mass in g (PDM) and the 21 numbers of green leaves (NGL), senescent leaves (NSL) and microcuttings (NM) were evaluated. The variables that best explained the observed behavior of the ?Florida Rough? lemon plants were NGL and PH, with values of 61.63 and 35.08%, respectively. The original concentration of the WPM with the addition of 25 g. L-1 of sucrose yielded the best ?Florida Rough? lemon plant growth reduction in vitro while maintaining the physiologically health of the plants.
650 $aCultura In Vitro
650 $aLimão
700 1 $aSOUZA, A. da S.
700 1 $aEMANUELA BARBOSA SANTOS
700 1 $aSOARES FILHO, W. dos S.
700 1 $aLEDO, C. A. da S.
700 1 $aCOSTA, E. M. R.
700 1 $aSOUZA, F. V. D.
773 $tCiência Rural, Santa Maria$gv.52:12, e20210276, 2022.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Classificação |
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