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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Cerrados. |
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Data corrente: |
16/05/2019 |
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Data da última atualização: |
13/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOZZAQUATRO, J. O.; MELLO, D. C.; OLIVEIRA, R. C. S.; ROSA, R. C. C.; COSTA, A. M.; CALDAS, E. D. |
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Afiliação: |
JOSEANE O. MOZZAQUATRO, UnB; DENISE C. MELLO, UnB; REGINALDO C. S. OLIVEIRA, UnB; RAUL CASTRO CARRIELLO ROSA, CNPAB; ANA MARIA COSTA, CPAC; ELOISA D. CALDAS, UnB. |
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Título: |
Dithiocarbamate residues in fruits and leaves of passion fruit (Passiflora edulis) from different brazilian regions. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of the Brazilian Chemical Society, 2019. |
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DOI: |
0103-5053.20190091 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
Dithiocarbamates are widely used fungicides, including in passion fruit, whose fruits are mainly used for juice production, the leaves for the preparation of herbal tea and medicines. Also, the use of the peel in the food industry has been proposed. In this study, the spectrophotometric method for determination of dithiocarbamate residues, as CS2, in passion fruit (Passiflora edulis) was validated at a limit of quantification (LOQ) of 0.05 mg kg-1 CS2, and 108 samples (55 fruits and 53 leaves) collected from Brazilian growers were analyzed. About 25% of the fruit peel samples were positive (0.06 to 1.4 mg kg-1) and only one sample had residues in the pulp (0.09 mg kg-1), 43.4% of leaf samples contained residues. Washing of fruit reduced the residues in the peel by up to 100%, and drying the leaves increased residue levels by up to 60%. This is the first study that reports dithiocarbamate residues in passion fruit in Brazil, and the results are important for government authorities when planning monitoring programs, and for food and herbal medicine industries. |
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Thesagro: |
Casca; Folha; Fungicida; Herbicida Residual; Maracujá; Resíduo. |
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Thesaurus Nal: |
dithiocarbamate fungicides. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/197375/1/2019-0025AR.pdf
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Marc: |
LEADER 01883naa a2200277 a 4500
001 2109078
005 2020-01-13
008 2019 bl uuuu u00u1 u #d
024 7 $a0103-5053.20190091$2DOI
100 1 $aMOZZAQUATRO, J. O.
245 $aDithiocarbamate residues in fruits and leaves of passion fruit (Passiflora edulis) from different brazilian regions.$h[electronic resource]
260 $c2019
520 $aDithiocarbamates are widely used fungicides, including in passion fruit, whose fruits are mainly used for juice production, the leaves for the preparation of herbal tea and medicines. Also, the use of the peel in the food industry has been proposed. In this study, the spectrophotometric method for determination of dithiocarbamate residues, as CS2, in passion fruit (Passiflora edulis) was validated at a limit of quantification (LOQ) of 0.05 mg kg-1 CS2, and 108 samples (55 fruits and 53 leaves) collected from Brazilian growers were analyzed. About 25% of the fruit peel samples were positive (0.06 to 1.4 mg kg-1) and only one sample had residues in the pulp (0.09 mg kg-1), 43.4% of leaf samples contained residues. Washing of fruit reduced the residues in the peel by up to 100%, and drying the leaves increased residue levels by up to 60%. This is the first study that reports dithiocarbamate residues in passion fruit in Brazil, and the results are important for government authorities when planning monitoring programs, and for food and herbal medicine industries.
650 $adithiocarbamate fungicides
650 $aCasca
650 $aFolha
650 $aFungicida
650 $aHerbicida Residual
650 $aMaracujá
650 $aResíduo
700 1 $aMELLO, D. C.
700 1 $aOLIVEIRA, R. C. S.
700 1 $aROSA, R. C. C.
700 1 $aCOSTA, A. M.
700 1 $aCALDAS, E. D.
773 $tJournal of the Brazilian Chemical Society, 2019.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Meio-Norte. |
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Data corrente: |
02/09/2021 |
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Data da última atualização: |
02/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FERREIRA, T. M. M.; LEAO, A. P.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. |
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Afiliação: |
THALITA M. M. FERREIRA, Universidade Federal de Lavras; ANDRE PEREIRA LEAO, CNPAE; CARLOS ANTONIO FERREIRA DE SOUSA, CPAMN; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
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Título: |
Genes highly overexpressed in salt-stressed young oil palm (Elaeis guineensis) plants. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Revista Brasileira de Engenharia Agrícola e Ambiental, Campina Grande, v. 25, n. 12, p. 813-818, 2021. |
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ISSN: |
1807-1929 |
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DOI: |
http://dx.doi.org/10.1590/1807-1929/agriambi.v25n12p813-818 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Genes altamente superexpressos em dendezeiros jovens (Elaeis guineensis) estressados por sal. |
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Conteúdo: |
Abstract: RNA-seq is a technique based on the large-scale sequencing of transcript-derived cDNAs using next-generation sequencing platforms mostly used today to characterize an organism?s transcriptome. The analysis of RNA-seq data allows for identifying genes differentially expressed in a given condition, such as salt stress. This study aimed to search and characterize genes from the African oil palm (Elaeis guineensis Jacq.) highly up-regulated during salt stress, with a long-term goal of gene promoter prospection and validation. The apical leaves from the control (electrical conductivity of ~2 dS m-1) and salt-stressed (~40 dS m-1) young oil palm plants, collected at 5 and 12 days after the beginning of the stress, were subjected to extraction of total RNA, with three plants (replicates) per treatment. The complete genome of E. guineensis, available at the National Center for Biotechnology Information, was used as the reference genome - BioProject PRJNA192219. The differential expression analysis led to the selection for further characterization of seven genes, which had increased expressions of 37?84 times under salt stress. The strategy used in this study enabled the selection of seven salt-responsive genes highly up-regulated during salt stress, and some of them coded for proteins already reported as responsible for salinity tolerance in other plant species through over-expression or knockout. Resumo: O RNA-seq é uma técnica baseada no sequenciamento em larga escala de cDNAs derivados de transcritos - por meio de plataformas de sequenciamento de nova geração ? amplamente utilizada atualmente para caracterizar o transcriptoma de um organismo. A análise dos dados de RNA-seq permite identificar genes diferencialmente expressos em uma determinada condição, como o estresse salino. Este estudo teve como objetivo prospectar genes do dendê (Elaeis guineensis) responsivos ao estresse salino e realizar sua caracterização funcional e estrutural. A folha apical de plantas jovens de dendê controle (condutividade elétrica de ~2 dS m-1) e estressadas (~40 dS m-1), coletadas aos 5 e 12 dias após o início do estresse, foi submetida à extração de RNA total, com três plantas (repetições) por tratamento. O genoma completo de E. guineensis, disponível no ?National Center for Biotechnology Information?, foi utilizado como genoma de referência - BioProject PRJNA192219. A análise da expressão diferencial levou à seleção de sete genes, cujo nível de expressão aumentou entre 37 e 84 vezes sob estresse salino, para posterior caracterização. A estratégia utilizada neste estudo permitiu a seleção de sete genes responsivos ao sal suprarregulados durante o estresse, e alguns deles codificam proteínas já relatadas como responsáveis pela tolerância à salinidade em outras espécies de plantas por superexpressão ou knock-out. MenosAbstract: RNA-seq is a technique based on the large-scale sequencing of transcript-derived cDNAs using next-generation sequencing platforms mostly used today to characterize an organism?s transcriptome. The analysis of RNA-seq data allows for identifying genes differentially expressed in a given condition, such as salt stress. This study aimed to search and characterize genes from the African oil palm (Elaeis guineensis Jacq.) highly up-regulated during salt stress, with a long-term goal of gene promoter prospection and validation. The apical leaves from the control (electrical conductivity of ~2 dS m-1) and salt-stressed (~40 dS m-1) young oil palm plants, collected at 5 and 12 days after the beginning of the stress, were subjected to extraction of total RNA, with three plants (replicates) per treatment. The complete genome of E. guineensis, available at the National Center for Biotechnology Information, was used as the reference genome - BioProject PRJNA192219. The differential expression analysis led to the selection for further characterization of seven genes, which had increased expressions of 37?84 times under salt stress. The strategy used in this study enabled the selection of seven salt-responsive genes highly up-regulated during salt stress, and some of them coded for proteins already reported as responsible for salinity tolerance in other plant species through over-expression or knockout. Resumo: O RNA-seq é uma técnica baseada no sequenciamento em larga escala de... Mostrar Tudo |
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Palavras-Chave: |
Análise de expressão diferencial; Differential expression analysis; Estresse salino; Genes responsivos ao sal; RNAseq; Salt-responsive genes; Transcriptômica. |
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Thesagro: |
Dendê; Elaeis Guineensis; Gene; RNA. |
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Thesaurus NAL: |
Salt stress; Transcriptomics. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/225681/1/Genes-highly-overexpressed-in-salt-stressed-2021.pdf
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Marc: |
LEADER 04093naa a2200349 a 4500
001 2134026
005 2021-09-02
008 2021 bl uuuu u00u1 u #d
022 $a1807-1929
024 7 $ahttp://dx.doi.org/10.1590/1807-1929/agriambi.v25n12p813-818$2DOI
100 1 $aFERREIRA, T. M. M.
245 $aGenes highly overexpressed in salt-stressed young oil palm (Elaeis guineensis) plants.$h[electronic resource]
260 $c2021
500 $aTítulo em português: Genes altamente superexpressos em dendezeiros jovens (Elaeis guineensis) estressados por sal.
520 $aAbstract: RNA-seq is a technique based on the large-scale sequencing of transcript-derived cDNAs using next-generation sequencing platforms mostly used today to characterize an organism?s transcriptome. The analysis of RNA-seq data allows for identifying genes differentially expressed in a given condition, such as salt stress. This study aimed to search and characterize genes from the African oil palm (Elaeis guineensis Jacq.) highly up-regulated during salt stress, with a long-term goal of gene promoter prospection and validation. The apical leaves from the control (electrical conductivity of ~2 dS m-1) and salt-stressed (~40 dS m-1) young oil palm plants, collected at 5 and 12 days after the beginning of the stress, were subjected to extraction of total RNA, with three plants (replicates) per treatment. The complete genome of E. guineensis, available at the National Center for Biotechnology Information, was used as the reference genome - BioProject PRJNA192219. The differential expression analysis led to the selection for further characterization of seven genes, which had increased expressions of 37?84 times under salt stress. The strategy used in this study enabled the selection of seven salt-responsive genes highly up-regulated during salt stress, and some of them coded for proteins already reported as responsible for salinity tolerance in other plant species through over-expression or knockout. Resumo: O RNA-seq é uma técnica baseada no sequenciamento em larga escala de cDNAs derivados de transcritos - por meio de plataformas de sequenciamento de nova geração ? amplamente utilizada atualmente para caracterizar o transcriptoma de um organismo. A análise dos dados de RNA-seq permite identificar genes diferencialmente expressos em uma determinada condição, como o estresse salino. Este estudo teve como objetivo prospectar genes do dendê (Elaeis guineensis) responsivos ao estresse salino e realizar sua caracterização funcional e estrutural. A folha apical de plantas jovens de dendê controle (condutividade elétrica de ~2 dS m-1) e estressadas (~40 dS m-1), coletadas aos 5 e 12 dias após o início do estresse, foi submetida à extração de RNA total, com três plantas (repetições) por tratamento. O genoma completo de E. guineensis, disponível no ?National Center for Biotechnology Information?, foi utilizado como genoma de referência - BioProject PRJNA192219. A análise da expressão diferencial levou à seleção de sete genes, cujo nível de expressão aumentou entre 37 e 84 vezes sob estresse salino, para posterior caracterização. A estratégia utilizada neste estudo permitiu a seleção de sete genes responsivos ao sal suprarregulados durante o estresse, e alguns deles codificam proteínas já relatadas como responsáveis pela tolerância à salinidade em outras espécies de plantas por superexpressão ou knock-out.
650 $aSalt stress
650 $aTranscriptomics
650 $aDendê
650 $aElaeis Guineensis
650 $aGene
650 $aRNA
653 $aAnálise de expressão diferencial
653 $aDifferential expression analysis
653 $aEstresse salino
653 $aGenes responsivos ao sal
653 $aRNAseq
653 $aSalt-responsive genes
653 $aTranscriptômica
700 1 $aLEAO, A. P.
700 1 $aSOUSA, C. A. F. de
700 1 $aSOUZA JUNIOR, M. T.
773 $tRevista Brasileira de Engenharia Agrícola e Ambiental, Campina Grande$gv. 25, n. 12, p. 813-818, 2021.
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Embrapa Agroenergia (CNPAE) |
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