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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
04/11/2014 |
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Data da última atualização: |
04/11/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, D. R. de; ZAMBERLAM, C. R.; GAIARDO, R. B.; REGO, G. M.; CERUTTI, J. M.; CAVALHEIRO, A. J.; CERUTTI, S. M. |
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Afiliação: |
Daniela Rodrigues de Oliveira, Universidade Federal de Sao Paulo; Cláudia R. Zamberlam, Universidade Federal de Sao Paulo; Renan Barreta Gaiardo, Universidade Federal de Sao Paulo; GIZELDA MAIA REGO, CNPF; Janete M. Cerutti, Universidade Federal de Sao Paulo; Alberto J. Cavalheiro, UNESP; Suzete M. Cerutti, Universidade Federal de Sao Paulo. |
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Título: |
Flavones from Erythrina falcata are modulators of fear memory. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Complementary and Alternative Medicine, v. 14, n. 288, p. 1-17, Aug. 2014. |
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DOI: |
10.1186/1472-6882-14-288 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Flavonoids, which have been identified in a variety of plants, have been demonstrated to elicit beneficial effects on memory. Some studies have reported that flavonoids derived from Erythrina plants can provide such beneficial effects on memory. The aim of this study was to identify the flavonoids present in the stem bark crude extract of Erythrina falcata (CE) and to perform a bioactivity-guided study on conditioned fear memory. Methods: The secondary metabolites of CE were identified by high performance liquid chromatography combined with a diode array detector, electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MSn) and nuclear magnetic resonance (NMR). The buthanolic fraction (BuF) was obtained by partitioning. Subfractions from BuF (BuF1 ? BuF6) and fraction flavonoidic (FfA and FfB) were obtained by flash chromatography. The BuF3 and BuF4 fractions were used for the isolation of flavonoids, which was performed using HPLC-PAD. The isolated substances were quantified by HPLC-DAD and their structures were confirmed by nuclear magnetic resonance (NMR). The activities of CE and the subfractions were monitored using a one-trial, step-down inhibitory avoidance (IA) task to identify the effects of these substances on the acquisition and extinction of conditioned fear in rats. Results: Six subclasses of flavonoids were identified for the first time in CE. According to our behavioral data, CE, BuF, BuF3 and BuF4, the flavonoidic fractions, vitexin, isovitexin and 6-C-glycoside-diosmetin improved the acquisition of fear memory. Rats treated with BuF, BuF3 and BuF4 were particularly resistant to extinction. Nevertheless, rats treated with FfA and FfB, vitexin, isovitexin and 6-C-glycoside-diosmetin exhibited gradual reduction in conditioned fear response during the extinction retest session, which was measured at 48 to 480 h after conditioning. Conclusions: Our results demonstrate that vitexin, isovitexin and diosmetin-6-C-glucoside and flavonoidic fractions resulted in a significant retention of fear memory but did not prevent the extinction of fear memory. These results further substantiate that the treatment with pure flavonoids or flavanoid-rich fractions might represent potential therapeutic approaches for the treatment of neurocognitive disorders, improvement of memory acquisition and spontaneous recovery of fear. MenosBackground: Flavonoids, which have been identified in a variety of plants, have been demonstrated to elicit beneficial effects on memory. Some studies have reported that flavonoids derived from Erythrina plants can provide such beneficial effects on memory. The aim of this study was to identify the flavonoids present in the stem bark crude extract of Erythrina falcata (CE) and to perform a bioactivity-guided study on conditioned fear memory. Methods: The secondary metabolites of CE were identified by high performance liquid chromatography combined with a diode array detector, electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MSn) and nuclear magnetic resonance (NMR). The buthanolic fraction (BuF) was obtained by partitioning. Subfractions from BuF (BuF1 ? BuF6) and fraction flavonoidic (FfA and FfB) were obtained by flash chromatography. The BuF3 and BuF4 fractions were used for the isolation of flavonoids, which was performed using HPLC-PAD. The isolated substances were quantified by HPLC-DAD and their structures were confirmed by nuclear magnetic resonance (NMR). The activities of CE and the subfractions were monitored using a one-trial, step-down inhibitory avoidance (IA) task to identify the effects of these substances on the acquisition and extinction of conditioned fear in rats. Results: Six subclasses of flavonoids were identified for the first time in CE. According to our behavioral data, CE, BuF, BuF3 and BuF4, the flavonoidic fractions, vitexin, isovit... Mostrar Tudo |
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Palavras-Chave: |
Acquisition; Fear conditioning; Flavona; Flavone; Medo condicionado; Memória; Uso terapêutico. |
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Thesagro: |
Erythrina Falcata. |
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Thesaurus Nal: |
extinction. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/110975/1/2014-API-Gizelda-FlavonesErythrina.pdf
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Marc: |
LEADER 03268naa a2200313 a 4500
001 1999163
005 2014-11-04
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1186/1472-6882-14-288$2DOI
100 1 $aOLIVEIRA, D. R. de
245 $aFlavones from Erythrina falcata are modulators of fear memory.$h[electronic resource]
260 $c2014
520 $aBackground: Flavonoids, which have been identified in a variety of plants, have been demonstrated to elicit beneficial effects on memory. Some studies have reported that flavonoids derived from Erythrina plants can provide such beneficial effects on memory. The aim of this study was to identify the flavonoids present in the stem bark crude extract of Erythrina falcata (CE) and to perform a bioactivity-guided study on conditioned fear memory. Methods: The secondary metabolites of CE were identified by high performance liquid chromatography combined with a diode array detector, electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MSn) and nuclear magnetic resonance (NMR). The buthanolic fraction (BuF) was obtained by partitioning. Subfractions from BuF (BuF1 ? BuF6) and fraction flavonoidic (FfA and FfB) were obtained by flash chromatography. The BuF3 and BuF4 fractions were used for the isolation of flavonoids, which was performed using HPLC-PAD. The isolated substances were quantified by HPLC-DAD and their structures were confirmed by nuclear magnetic resonance (NMR). The activities of CE and the subfractions were monitored using a one-trial, step-down inhibitory avoidance (IA) task to identify the effects of these substances on the acquisition and extinction of conditioned fear in rats. Results: Six subclasses of flavonoids were identified for the first time in CE. According to our behavioral data, CE, BuF, BuF3 and BuF4, the flavonoidic fractions, vitexin, isovitexin and 6-C-glycoside-diosmetin improved the acquisition of fear memory. Rats treated with BuF, BuF3 and BuF4 were particularly resistant to extinction. Nevertheless, rats treated with FfA and FfB, vitexin, isovitexin and 6-C-glycoside-diosmetin exhibited gradual reduction in conditioned fear response during the extinction retest session, which was measured at 48 to 480 h after conditioning. Conclusions: Our results demonstrate that vitexin, isovitexin and diosmetin-6-C-glucoside and flavonoidic fractions resulted in a significant retention of fear memory but did not prevent the extinction of fear memory. These results further substantiate that the treatment with pure flavonoids or flavanoid-rich fractions might represent potential therapeutic approaches for the treatment of neurocognitive disorders, improvement of memory acquisition and spontaneous recovery of fear.
650 $aextinction
650 $aErythrina Falcata
653 $aAcquisition
653 $aFear conditioning
653 $aFlavona
653 $aFlavone
653 $aMedo condicionado
653 $aMemória
653 $aUso terapêutico
700 1 $aZAMBERLAM, C. R.
700 1 $aGAIARDO, R. B.
700 1 $aREGO, G. M.
700 1 $aCERUTTI, J. M.
700 1 $aCAVALHEIRO, A. J.
700 1 $aCERUTTI, S. M.
773 $tBMC Complementary and Alternative Medicine$gv. 14, n. 288, p. 1-17, Aug. 2014.
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Registro original: |
Embrapa Florestas (CNPF) |
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| 1. |  | OLIVEIRA, C. J. P. de; OLIVEIRA, L. F. C. de; SILVA, A. G.; CABRERA DIAZ, J. L.; KUSDRA, G. do R. F.; OLIVEIRA, M. G. de C. Desempenho produtivo da cultivar de feijão BRS Esteio em unidades demonstrativas na região Centro-Sul do Paraná, 2015/2016. In: SEMINÁRIO JOVENS TALENTOS, 11., 2017, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2017. p. 47. (Embrapa Arroz e Feijão. Documentos, 316).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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