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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
19/09/2022 |
Data da última atualização: |
19/09/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MATTOS, L. M.; REIS, F. C.; RACANICCI, A. M. C.; PIVATO, I.; TONELLI, G. S. S. S.; BÁO, S. N.; MARTINS, C. F. |
Afiliação: |
LUCIANA MIRANDA MATTOS; FILIPE CARNEIRO REIS; ALINE M.C. RACANICCI; IVO PIVATO; GUILHERME S.S.S. TONELLI; SÔNIA NAIR BÁO; CARLOS FREDERICO MARTINS, CPAC. |
Título: |
Freezing for the Future: obtaining fibroblast samples from deceased wild mammals for the Brazilian Cerrado Germplasm Bank. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Biopreservation and Biobanking, ahead of print, 2022. |
DOI: |
DOI: 10.1089/bio.2022.0039 |
Idioma: |
Inglês |
Conteúdo: |
We isolated and further characterized fibroblasts obtained from postmortem skin biopsies of three different Brazilian wild species (Chrysocyon brachyurus?maned wolf, Cerdocyon thous?crab-eating fox, Mazama gouazoubira?brown brocket deer). The effects of two cryoprotectants, 10% dimethyl sulfoxide (DMSO) and 5% dimethylformamide (DMF), were assessed to determine the most efficient cryopreservation protocol. Such an investigation promotes the creation of germplasm banks, using samples that would otherwise be rejected and permanently lost following the death of the animals. We utilized animal corpses that were involved in highway accidents, found dead in the natural environment, or referred to us from the veterinary hospital at the Brası ́lia Zoo. Fibroblasts from C. brachyurus specimens presented a delay in cell growth in Dulbecco?s modified Eagle?s medium in relation to other species. This observation is a limiting factor for the future storage of cells from this species. Differences in cellular morphology were observed between C. brachyurus, C. thous, and M. goua- zoubira, presenting branched, fusiform, and spherical forms, respectively. The cryoprotective solution con- taining 10% DMSO was more efficient than 5% DMF medium in preserving the viability of fibroblasts of the three species ( p < 0.05). After defining the best cryopreservation solution, a germplasm bank was successfully formed. This biological reservoir is configured as the first germplasm bank containing somatic cells and gametes of wild mammals of the Cerrado biome of Brazil. This material will be used for future characterization of the species and multiplication by means of nuclear transfer cloning. MenosWe isolated and further characterized fibroblasts obtained from postmortem skin biopsies of three different Brazilian wild species (Chrysocyon brachyurus?maned wolf, Cerdocyon thous?crab-eating fox, Mazama gouazoubira?brown brocket deer). The effects of two cryoprotectants, 10% dimethyl sulfoxide (DMSO) and 5% dimethylformamide (DMF), were assessed to determine the most efficient cryopreservation protocol. Such an investigation promotes the creation of germplasm banks, using samples that would otherwise be rejected and permanently lost following the death of the animals. We utilized animal corpses that were involved in highway accidents, found dead in the natural environment, or referred to us from the veterinary hospital at the Brası ́lia Zoo. Fibroblasts from C. brachyurus specimens presented a delay in cell growth in Dulbecco?s modified Eagle?s medium in relation to other species. This observation is a limiting factor for the future storage of cells from this species. Differences in cellular morphology were observed between C. brachyurus, C. thous, and M. goua- zoubira, presenting branched, fusiform, and spherical forms, respectively. The cryoprotective solution con- taining 10% DMSO was more efficient than 5% DMF medium in preserving the viability of fibroblasts of the three species ( p < 0.05). After defining the best cryopreservation solution, a germplasm bank was successfully formed. This biological reservoir is configured as the first germplasm bank contain... Mostrar Tudo |
Thesagro: |
Animal Selvagem; Banco de Germoplasma; Biotecnologia; Criopreservação. |
Thesaurus Nal: |
Biotechnology; Cryopreservation; Germplasm; Wild animals. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02613naa a2200301 a 4500 001 2146609 005 2022-09-19 008 2022 bl uuuu u00u1 u #d 024 7 $aDOI: 10.1089/bio.2022.0039$2DOI 100 1 $aMATTOS, L. M. 245 $aFreezing for the Future$bobtaining fibroblast samples from deceased wild mammals for the Brazilian Cerrado Germplasm Bank.$h[electronic resource] 260 $c2022 520 $aWe isolated and further characterized fibroblasts obtained from postmortem skin biopsies of three different Brazilian wild species (Chrysocyon brachyurus?maned wolf, Cerdocyon thous?crab-eating fox, Mazama gouazoubira?brown brocket deer). The effects of two cryoprotectants, 10% dimethyl sulfoxide (DMSO) and 5% dimethylformamide (DMF), were assessed to determine the most efficient cryopreservation protocol. Such an investigation promotes the creation of germplasm banks, using samples that would otherwise be rejected and permanently lost following the death of the animals. We utilized animal corpses that were involved in highway accidents, found dead in the natural environment, or referred to us from the veterinary hospital at the Brası ́lia Zoo. Fibroblasts from C. brachyurus specimens presented a delay in cell growth in Dulbecco?s modified Eagle?s medium in relation to other species. This observation is a limiting factor for the future storage of cells from this species. Differences in cellular morphology were observed between C. brachyurus, C. thous, and M. goua- zoubira, presenting branched, fusiform, and spherical forms, respectively. The cryoprotective solution con- taining 10% DMSO was more efficient than 5% DMF medium in preserving the viability of fibroblasts of the three species ( p < 0.05). After defining the best cryopreservation solution, a germplasm bank was successfully formed. This biological reservoir is configured as the first germplasm bank containing somatic cells and gametes of wild mammals of the Cerrado biome of Brazil. This material will be used for future characterization of the species and multiplication by means of nuclear transfer cloning. 650 $aBiotechnology 650 $aCryopreservation 650 $aGermplasm 650 $aWild animals 650 $aAnimal Selvagem 650 $aBanco de Germoplasma 650 $aBiotecnologia 650 $aCriopreservação 700 1 $aREIS, F. C. 700 1 $aRACANICCI, A. M. C. 700 1 $aPIVATO, I. 700 1 $aTONELLI, G. S. S. S. 700 1 $aBÁO, S. N. 700 1 $aMARTINS, C. F. 773 $tBiopreservation and Biobanking, ahead of print, 2022.
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Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Tabuleiros Costeiros. |
Data corrente: |
30/03/2016 |
Data da última atualização: |
05/09/2022 |
Tipo da produção científica: |
Documentos |
Autoria: |
RODRIGUES, R. F. de A.; MOTA, D. M. da; SILVA JUNIOR, J. F. da; VIEIRA, D. L. M.; PEREIRA, E. O.; BRITO, J. V. dos S.; JESUS, N. S. de. |
Afiliação: |
RAQUEL FERNANDES DE A RODRIGUES, CPATC; DALVA MARIA DA MOTA, CPATU; JOSUE FRANCISCO DA SILVA JUNIOR, CPATC; DANIEL LUIS MASCIA VIEIRA, CENARGEN; EMANUEL OLIVEIRA PEREIRA; JANE VELMA DOS SANTOS BRITO; NADIA SANTOS DE JESUS. |
Título: |
As catadoras de mangaba em defesa dos seus modos de vida. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Aracaju: Embrapa Tabuleiros Costeiros, 2015. |
Páginas: |
55 p. |
Série: |
(Embrapa Tabuleiros Costeiros. Documentos, 192). |
ISSN: |
1678-1953 |
Idioma: |
Português |
Conteúdo: |
Este documento registra as atividades e conteúdos de acordo com a ordem cronológica do II Encontro das Catadoras de Mangaba. Por fim, os autores refletem sobre as experiências de acesso aos recursos e político-institucionais do público extrativista presente, frente à conservação das áreas de ocorrência da mangabeira e modos de vidas das comunidades locais envolvidas com a coleta da mangaba em Sergipe. |
Palavras-Chave: |
Catador; Extrativismo; Moso de vida; Sergipe. |
Thesagro: |
Comunidade Rural; Fruta tropical; Mangaba; Mulher Rural. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141804/1/DOC-192.pdf
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Marc: |
LEADER 01287nam a2200313 a 4500 001 2042163 005 2022-09-05 008 2015 bl uuuu 00u1 u #d 022 $a1678-1953 100 1 $aRODRIGUES, R. F. de A. 245 $aAs catadoras de mangaba em defesa dos seus modos de vida.$h[electronic resource] 260 $aAracaju: Embrapa Tabuleiros Costeiros$c2015 300 $a55 p. 490 $a(Embrapa Tabuleiros Costeiros. Documentos, 192). 520 $aEste documento registra as atividades e conteúdos de acordo com a ordem cronológica do II Encontro das Catadoras de Mangaba. Por fim, os autores refletem sobre as experiências de acesso aos recursos e político-institucionais do público extrativista presente, frente à conservação das áreas de ocorrência da mangabeira e modos de vidas das comunidades locais envolvidas com a coleta da mangaba em Sergipe. 650 $aComunidade Rural 650 $aFruta tropical 650 $aMangaba 650 $aMulher Rural 653 $aCatador 653 $aExtrativismo 653 $aMoso de vida 653 $aSergipe 700 1 $aMOTA, D. M. da 700 1 $aSILVA JUNIOR, J. F. da 700 1 $aVIEIRA, D. L. M. 700 1 $aPEREIRA, E. O. 700 1 $aBRITO, J. V. dos S. 700 1 $aJESUS, N. S. de
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