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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
12/09/2016 |
Data da última atualização: |
13/04/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SA, F. P. de; LEDO, A. da S.; AMORIM, J. A. E.; MUNIZ, A. V. C. da S.; PASQUAL, M. |
Afiliação: |
FRANCIELEN PAOLA DE SA; ANA DA SILVA LEDO, CPATC; JULIE ANNE ESPINDOLA AMORIM; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; MOACIR PASCOAL. |
Título: |
In vitro propagation and acclimatization of genipapo accessions. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Ciência e Agrotecnologia, Lavras, MG, v. 40, n. 2, p. 155-163, mar./abr. 2016. |
Idioma: |
Inglês |
Conteúdo: |
Genipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions was assessed. The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Culture media supplementation with 1.0 mg L-1 BAP favors the development of adventitious shoots and promotes a higher callogenesis percentage, leaf number and feasibility at 90 days of in vitro culture. The immersion of the basal portion of shoots for 20 seconds in all IBA solutions induces the rhizogenesis in the genipapo accessions at 90 days of in vitro culture. The substrates consisting of washed sand + dried coconut shell powder, at a 1:1 ratio by volume, and Topstrato HT® may be recommended for genipapo acclimatization. The NB accession is genetically different from the CZA and AS populations and shows superiority for most of the variables analyzed. MenosGenipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions was assessed. The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Culture media supplementation with 1.0 mg L-1 BAP favors the development of adventitious shoots and promotes a higher callogenesis percentage, leaf number and feasibility at 90 days of in vitro culture. The immersion of the basal portion of shoots for 20 seconds in all IBA solutions induces the rhizogenesis in the genipapo accessions at 90 days of in vitro culture. The substrates consisting of washed sand + dried coconut shell powder, at a 1:1 ratio by volume, and Topstrato ... Mostrar Tudo |
Palavras-Chave: |
Genética de planta; Genipapo. |
Thesagro: |
Fruta tropical; Jenipapo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02322naa a2200217 a 4500 001 2052710 005 2017-04-13 008 2016 bl uuuu u00u1 u #d 100 1 $aSA, F. P. de 245 $aIn vitro propagation and acclimatization of genipapo accessions.$h[electronic resource] 260 $c2016 520 $aGenipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions was assessed. The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Culture media supplementation with 1.0 mg L-1 BAP favors the development of adventitious shoots and promotes a higher callogenesis percentage, leaf number and feasibility at 90 days of in vitro culture. The immersion of the basal portion of shoots for 20 seconds in all IBA solutions induces the rhizogenesis in the genipapo accessions at 90 days of in vitro culture. The substrates consisting of washed sand + dried coconut shell powder, at a 1:1 ratio by volume, and Topstrato HT® may be recommended for genipapo acclimatization. The NB accession is genetically different from the CZA and AS populations and shows superiority for most of the variables analyzed. 650 $aFruta tropical 650 $aJenipapo 653 $aGenética de planta 653 $aGenipapo 700 1 $aLEDO, A. da S. 700 1 $aAMORIM, J. A. E. 700 1 $aMUNIZ, A. V. C. da S. 700 1 $aPASQUAL, M. 773 $tCiência e Agrotecnologia, Lavras, MG$gv. 40, n. 2, p. 155-163, mar./abr. 2016.
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| Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
14/03/2005 |
Data da última atualização: |
21/08/2007 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Circulação/Nível: |
-- - -- |
Autoria: |
SANTOS, A. B. dos; SILVA, S. C. da; ZIMMERMANN, F. J. P. |
Título: |
Efeitos do manejo da irrigação na temperatura da água e no comportamento do arroz irrigado. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 3.; REUNIÃO DA CULTURA DO ARROZ IRRIGADO, 25., 2003, Balneário Camboriú. Anais... Balneário Camboriú: EPAGRI, 2003. |
Páginas: |
p. 181-183. |
Idioma: |
Português |
Conteúdo: |
O objetivo deste estudo foi determinar os efeitos de manejos de água sobre a temperatura da água de irrigação e o comportamento de genótipos de arroz irrigado. |
Thesagro: |
Arroz; Irrigação; Manejo; Oryza Sativa; Temperatura. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00877naa a2200217 a 4500 001 1213076 005 2007-08-21 008 2003 bl uuuu u00u1 u #d 100 1 $aSANTOS, A. B. dos 245 $aEfeitos do manejo da irrigação na temperatura da água e no comportamento do arroz irrigado. 260 $c2003 300 $ap. 181-183. 520 $aO objetivo deste estudo foi determinar os efeitos de manejos de água sobre a temperatura da água de irrigação e o comportamento de genótipos de arroz irrigado. 650 $aArroz 650 $aIrrigação 650 $aManejo 650 $aOryza Sativa 650 $aTemperatura 700 1 $aSILVA, S. C. da 700 1 $aZIMMERMANN, F. J. P. 773 $tIn: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 3.; REUNIÃO DA CULTURA DO ARROZ IRRIGADO, 25., 2003, Balneário Camboriú. Anais... Balneário Camboriú: EPAGRI, 2003.
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