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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
15/08/2016 |
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Data da última atualização: |
02/12/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHAVES, J. da S.; BARAÚNA, A. C.; MOSQUEIRA, C. A.; GIANLUPPI, V.; ZILLI, J. E.; SILVA, K. da. |
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Afiliação: |
JOSIMAR DA SILVA CHAVES, UFRR; ALEXANDRE CARDOSO BARAÚNA, UFRRJ; CÁTIA APARECIDA MOSQUEIRA, UFRR; VICENTE GIANLUPPI, CPAF-Roraima; JERRI EDSON ZILLI, CNPAB; KRISLE DA SILVA, CPAF-Roraima. |
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Título: |
Stylosanthes spp. from Amazon savanna harbour diverse and potentially effective rhizobia. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Applied Soil Ecology, v. 108, p. 54-61, 2016 |
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Idioma: |
Inglês |
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Conteúdo: |
The Stylosanthes genus stands out among the forage legume species well-adapted to the soil and climatic conditions of tropical regions. This legume establishes a symbiotic relationship with nitrogen-fixing bacteria, which can be used to improve the quality of pastures. However, there are few studies about the diversity and symbiotic efficiency of nitrogen-fixing bacteria in Stylosanthes plants. Therefore, the objective was to isolate, characterize and evaluate the symbiotic efficiency of nitrogen-fixing bacteria in symbiosis with Stylosanthes in savanna areas in Roraima, Brazil. Plants with nodules were collected at 6 sites, including cultivated and native areas of the savanna. Shoots of Stylosanthes with flowers were collected to prepare exsiccatae for species identification. The nodules were surface-sterilized with 5% sodium hypochlorite after the roots were washed, and isolation and morphological characterization were performed in 79 culture medium. The isolates were selected according to their morphology, the representativeness of each collection site and the associated Stylosanthes species. Representative isolates were tested for nodulation in cowpea plants, and the isolates able to nodulate the gene 16S rRNA was sequenced. The symbiotic efficiency was evaluated in Stylosanthes capitata cv. Lavradeiro in greenhouse conditions for 65 days. A total of 504 nodules were collected, from which 258 pure isolates were obtained from 5 species of Stylosanthes (S. capitata,S. scabra, S. gracilis, S. humilis and S. angustifolia). The isolates showed significant phenotypic diversity, and 89 isolates were selected. Thirty-one isolates were confirmed to be nodulating bacteria in cowpea plants. These isolates were identified as a-Proteobacteria, with Bradyrhizobium, rhizobium and Mesorhizobium genera, and b-Proteobacteria, with Burkholderia and Herbaspirillum genera. When evaluated in S. capitata, treatment with two Bradyrhizobium isolates, ERR 1178 and ERR 942, resulted in significant production of plant biomass production and N accumulation. These results indicated that a large diversity of a-Proteobacteria and b-Proteobacteria are able to nodulate Stylosanthes spp. and some isolates have potential for testing under
field conditions. MenosThe Stylosanthes genus stands out among the forage legume species well-adapted to the soil and climatic conditions of tropical regions. This legume establishes a symbiotic relationship with nitrogen-fixing bacteria, which can be used to improve the quality of pastures. However, there are few studies about the diversity and symbiotic efficiency of nitrogen-fixing bacteria in Stylosanthes plants. Therefore, the objective was to isolate, characterize and evaluate the symbiotic efficiency of nitrogen-fixing bacteria in symbiosis with Stylosanthes in savanna areas in Roraima, Brazil. Plants with nodules were collected at 6 sites, including cultivated and native areas of the savanna. Shoots of Stylosanthes with flowers were collected to prepare exsiccatae for species identification. The nodules were surface-sterilized with 5% sodium hypochlorite after the roots were washed, and isolation and morphological characterization were performed in 79 culture medium. The isolates were selected according to their morphology, the representativeness of each collection site and the associated Stylosanthes species. Representative isolates were tested for nodulation in cowpea plants, and the isolates able to nodulate the gene 16S rRNA was sequenced. The symbiotic efficiency was evaluated in Stylosanthes capitata cv. Lavradeiro in greenhouse conditions for 65 days. A total of 504 nodules were collected, from which 258 pure isolates were obtained from 5 species of Stylosanthes (S. capitata,S. scab... Mostrar Tudo |
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Palavras-Chave: |
16S rRNA; Biological nitrogen fixation; Eficiência simbiótica; FBN; Forragem tropical; Symbiotic efficiency; Tropical forage. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 03033naa a2200265 a 4500
001 2050888
005 2016-12-02
008 2016 bl --- 0-- u #d
100 1 $aCHAVES, J. da S.
245 $aStylosanthes spp. from Amazon savanna harbour diverse and potentially effective rhizobia.$h[electronic resource]
260 $c2016
520 $aThe Stylosanthes genus stands out among the forage legume species well-adapted to the soil and climatic conditions of tropical regions. This legume establishes a symbiotic relationship with nitrogen-fixing bacteria, which can be used to improve the quality of pastures. However, there are few studies about the diversity and symbiotic efficiency of nitrogen-fixing bacteria in Stylosanthes plants. Therefore, the objective was to isolate, characterize and evaluate the symbiotic efficiency of nitrogen-fixing bacteria in symbiosis with Stylosanthes in savanna areas in Roraima, Brazil. Plants with nodules were collected at 6 sites, including cultivated and native areas of the savanna. Shoots of Stylosanthes with flowers were collected to prepare exsiccatae for species identification. The nodules were surface-sterilized with 5% sodium hypochlorite after the roots were washed, and isolation and morphological characterization were performed in 79 culture medium. The isolates were selected according to their morphology, the representativeness of each collection site and the associated Stylosanthes species. Representative isolates were tested for nodulation in cowpea plants, and the isolates able to nodulate the gene 16S rRNA was sequenced. The symbiotic efficiency was evaluated in Stylosanthes capitata cv. Lavradeiro in greenhouse conditions for 65 days. A total of 504 nodules were collected, from which 258 pure isolates were obtained from 5 species of Stylosanthes (S. capitata,S. scabra, S. gracilis, S. humilis and S. angustifolia). The isolates showed significant phenotypic diversity, and 89 isolates were selected. Thirty-one isolates were confirmed to be nodulating bacteria in cowpea plants. These isolates were identified as a-Proteobacteria, with Bradyrhizobium, rhizobium and Mesorhizobium genera, and b-Proteobacteria, with Burkholderia and Herbaspirillum genera. When evaluated in S. capitata, treatment with two Bradyrhizobium isolates, ERR 1178 and ERR 942, resulted in significant production of plant biomass production and N accumulation. These results indicated that a large diversity of a-Proteobacteria and b-Proteobacteria are able to nodulate Stylosanthes spp. and some isolates have potential for testing under field conditions.
653 $a16S rRNA
653 $aBiological nitrogen fixation
653 $aEficiência simbiótica
653 $aFBN
653 $aForragem tropical
653 $aSymbiotic efficiency
653 $aTropical forage
700 1 $aBARAÚNA, A. C.
700 1 $aMOSQUEIRA, C. A.
700 1 $aGIANLUPPI, V.
700 1 $aZILLI, J. E.
700 1 $aSILVA, K. da
773 $tApplied Soil Ecology$gv. 108, p. 54-61, 2016
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
28/12/2006 |
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Data da última atualização: |
03/08/2019 |
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Tipo da produção científica: |
Artigo de Divulgação na Mídia |
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Autoria: |
MANDELLI, F.; ZAT, D. A. |
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Afiliação: |
FRANCISCO MANDELLI, CNPUV; DALTON ANTONIO ZAT, CNPUV. |
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Título: |
30 anos de observações meteorológicas. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Bon Vivant, Flores da Cunha, v. 8, n. 94, p. 8, 2006. |
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Idioma: |
Português |
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Conteúdo: |
Visando monitorar o comportamento das condições climáticas, a Embrapa Uva e Vinho de Bento Gonçalves-RS possui há 30 anos uma Estação Agroclimatológica, cujos trabalhos estão sendo coordenados pelo técnico Dalton Antonio Zat, desde 1976. A estação é integrante da rede do Instituto Nacional de Meteorologia e está subordinada ao 8° Distrito de Meteorologia, que tem sede em Porto Alegre e é o órgão coordenador para o Rio Grande do Sul, Santa Catarina e Paraná. |
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Palavras-Chave: |
Embrapa Uva e Vinho. |
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Thesagro: |
Clima; Historia; Uva; Viticultura. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/200228/1/8296-2006-p.8.pdf
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Marc: |
LEADER 00956nam a2200181 a 4500
001 1541560
005 2019-08-03
008 2006 bl uuuu u00u1 u #d
100 1 $aMANDELLI, F.
245 $a30 anos de observações meteorológicas.$h[electronic resource]
260 $aBon Vivant, Flores da Cunha, v. 8, n. 94, p. 8$c2006
520 $aVisando monitorar o comportamento das condições climáticas, a Embrapa Uva e Vinho de Bento Gonçalves-RS possui há 30 anos uma Estação Agroclimatológica, cujos trabalhos estão sendo coordenados pelo técnico Dalton Antonio Zat, desde 1976. A estação é integrante da rede do Instituto Nacional de Meteorologia e está subordinada ao 8° Distrito de Meteorologia, que tem sede em Porto Alegre e é o órgão coordenador para o Rio Grande do Sul, Santa Catarina e Paraná.
650 $aClima
650 $aHistoria
650 $aUva
650 $aViticultura
653 $aEmbrapa Uva e Vinho
700 1 $aZAT, D. A.
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Embrapa Uva e Vinho (CNPUV) |
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