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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
17/01/2008 |
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Data da última atualização: |
20/07/2018 |
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Autoria: |
COSTA, C. T. da; ALBUQUERQUE, A. C. S.; NASCIMENTO JUNIOR, A. do; MARCELINO-GUIMARÃES, F. C.; PEREIRA, J. F. |
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Afiliação: |
Cibele Tesser da Costa, CNPT; ANA CHRISTINA SAGEBIN ALBUQUERQUE, Embrapa Sede; ALFREDO DO NASCIMENTO JUNIOR, CNPT; FRANCISMAR CORREA MARCELINO GUIMARA, CNPSO; JORGE FERNANDO PEREIRA, CNPT. |
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Título: |
Genetic diversity of Brazilian triticales evaluated with genomic wheat microsatellites |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 11, p. 1577-1586, nov. 2007 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Diversidade genética de triticales brasileiros avaliada com microssatélites genômicos de trigo. |
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Conteúdo: |
The objective of this work was to determine the genetic variability available for triticale (X Triticosecale Wittmack) crop improvement in Brazil. Forty-two wheat genomic microsatellites were used to estimate the molecular diversity of 54 genotypes, which constitute the base of one of the major triticale breeding programs in the country. Average heterozygosity was 0.06 and average and effective number of alleles per locus were 2.13 and 1.61, respectively, with average allelic frequency of 0.34. The set of genomic wheat microsatellites used clustered the genotypes into seven groups, even when the germplasm was originated primarily from only two triticale breeding programs, a fact reflected on the average polymorphic information content value estimated for the germplasm (0.36). The 71.42% transferability achieved for the tested microsatellites indicates the possibility of exploiting these transferable markers in further triticale genetic and breeding studies, even those mapped on the D genome of wheat, when analyzing hexaploid triticales. |
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Palavras-Chave: |
freqüência alélica; frequency of alleles; heterozigosidade; índice de polimorfismo; number of alleles; número efetivo de alelos; polymorphism information content; transferabilidade; transferability; X Triticosecale. |
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Thesaurus Nal: |
heterozygosity. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/41162/1/42n11a09.pdf
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Marc: |
LEADER 02145naa a2200313 a 4500
001 1122827
005 2018-07-20
008 2007 bl uuuu u00u1 u #d
100 1 $aCOSTA, C. T. da
245 $aGenetic diversity of Brazilian triticales evaluated with genomic wheat microsatellites
260 $c2007
500 $aTítulo em português: Diversidade genética de triticales brasileiros avaliada com microssatélites genômicos de trigo.
520 $aThe objective of this work was to determine the genetic variability available for triticale (X Triticosecale Wittmack) crop improvement in Brazil. Forty-two wheat genomic microsatellites were used to estimate the molecular diversity of 54 genotypes, which constitute the base of one of the major triticale breeding programs in the country. Average heterozygosity was 0.06 and average and effective number of alleles per locus were 2.13 and 1.61, respectively, with average allelic frequency of 0.34. The set of genomic wheat microsatellites used clustered the genotypes into seven groups, even when the germplasm was originated primarily from only two triticale breeding programs, a fact reflected on the average polymorphic information content value estimated for the germplasm (0.36). The 71.42% transferability achieved for the tested microsatellites indicates the possibility of exploiting these transferable markers in further triticale genetic and breeding studies, even those mapped on the D genome of wheat, when analyzing hexaploid triticales.
650 $aheterozygosity
653 $afreqüência alélica
653 $afrequency of alleles
653 $aheterozigosidade
653 $aíndice de polimorfismo
653 $anumber of alleles
653 $anúmero efetivo de alelos
653 $apolymorphism information content
653 $atransferabilidade
653 $atransferability
653 $aX Triticosecale
700 1 $aALBUQUERQUE, A. C. S.
700 1 $aNASCIMENTO JUNIOR, A. do
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aPEREIRA, J. F.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 42, n. 11, p. 1577-1586, nov. 2007
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
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Data corrente: |
07/06/2023 |
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Data da última atualização: |
29/11/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
FERNANDEZ, Z.; RODRIGUES, R. de A.; TORRES, J. M.; MARCON, G. E. B.; FERREIRA, E. de C.; SOUZA, V. F. de; SARTI, E. F. B.; BERTOLLI, G. F.; ARAUJO, D.; DEMARCHI, L. H. F.; LICHS, G.; ZARDIN, M. U.; GONÇALVES, C. C. M.; CUENCA, V.; FAVACHO, A.; GUILHERMINO, J.; SANTOS, L. R. dos; ARAUJO, F. R.; SILVA, M. R. |
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Afiliação: |
ZORAIDA FERNANDEZ, Fundação Oswaldo Cruz; RUDIELLE DE ARRUDA RODRIGUES, Fundação Oswaldo Cruz; JAIRE MARINHO TORRES, Fundação Oswaldo Cruz; GLAUCIA ELISETE BARBOSA MARCON, Fundação Oswaldo Cruz; EDUARDO DE CASTRO FERREIRA, Fundação Oswaldo Cruz; VANESSA FELIPE DE SOUZA, CNPGC; ELAINE FERNANDES BAEZ SARTI, Universidade Federal de Mato Grosso do Sul; GUILHERME FERMINAO BERTOLLI, Universidade Federal de Mato Grosso do Sul; DANIEL ARAUJO, Universidade Federal de Mato Grosso do Sul; LUIZ HENRIQUE FERRAZ DEMARCHI, Laboratório Central de Saúde do Mato Grosso do Sul; GISLENE LICHS, Laboratório Central de Saúde do Mato Grosso do Sul; MARINA UMAKI ZARDIN, Laboratório Central de Saúde do Mato Grosso do Sul; CRHISTINNE CAVALHEIRO MAYMONE GONÇALVES, Secretaria de Estado de Saúde do Mato Grosso do Sul; VALTER CUENCA, Fundação Universidade Federal de Mato Grosso do Sul; ALEXSANDRA FAVACHO, Fundação Oswaldo Cruz; JISLAINE GUILHERMINO, Fundação Oswaldo Cruz; LENITA RAMIRES DOS SANTOS, CNPGC; FLABIO RIBEIRO DE ARAUJO, CNPGC; MARCIO ROBERTO SILVA, CNPGL. |
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Título: |
Development and validity assessment of ELISA test with recombinant chimeric protein of SARS-CoV-2. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Journal of Immunological Methods, v. 519, e113489, 2023. |
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DOI: |
https://doi.org/10.1016/j.jim.2023.113489 |
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Idioma: |
Inglês |
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Conteúdo: |
Serological tests developed for COVID-19 diagnostic are based on antibodies specific for SARS-CoV-2 antigens. Most of the antigens consist of a fragment or a whole amino acid sequence of the nucleocapsid or spike proteins. We evaluated a chimeric recombinant protein as an antigen in an ELISA test, using the most conserved and hydrophilic portions of the S1-subunit of the S and Nucleocapsid (N) proteins. These proteins, individually, indicated a suitable sensitivity of 93.6 and 100% and a specificity of 94.5 and 91.3%, respectively. However, our study with the chimera containing S1 and N proteins of SARS-CoV-2 suggested that the recombinant protein could better balance both the sensitivity (95.7%) and the specificity (95.5%) of the serological assay when comparing with the ELISA test using the antigens N and S1, individually. Accordingly, the chimera showed a high area under the ROC curve of 0.98 (CI 95% 0.958-1). Thus, our chimeric approach could be used to assess the natural exposure against SARS-CoV-2 virus over time, however, other tests will be necessary to better understand the behaviour of the chimera in samples from people with different vaccination doses and/or infected with different variants of the virus. |
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Palavras-Chave: |
Chimeric protein; COVID 19; Diagnostic; Proteína quimérica; Teste ELISA. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02413naa a2200409 a 4500
001 2154381
005 2023-11-29
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.jim.2023.113489$2DOI
100 1 $aFERNANDEZ, Z.
245 $aDevelopment and validity assessment of ELISA test with recombinant chimeric protein of SARS-CoV-2.$h[electronic resource]
260 $c2023
520 $aSerological tests developed for COVID-19 diagnostic are based on antibodies specific for SARS-CoV-2 antigens. Most of the antigens consist of a fragment or a whole amino acid sequence of the nucleocapsid or spike proteins. We evaluated a chimeric recombinant protein as an antigen in an ELISA test, using the most conserved and hydrophilic portions of the S1-subunit of the S and Nucleocapsid (N) proteins. These proteins, individually, indicated a suitable sensitivity of 93.6 and 100% and a specificity of 94.5 and 91.3%, respectively. However, our study with the chimera containing S1 and N proteins of SARS-CoV-2 suggested that the recombinant protein could better balance both the sensitivity (95.7%) and the specificity (95.5%) of the serological assay when comparing with the ELISA test using the antigens N and S1, individually. Accordingly, the chimera showed a high area under the ROC curve of 0.98 (CI 95% 0.958-1). Thus, our chimeric approach could be used to assess the natural exposure against SARS-CoV-2 virus over time, however, other tests will be necessary to better understand the behaviour of the chimera in samples from people with different vaccination doses and/or infected with different variants of the virus.
653 $aChimeric protein
653 $aCOVID 19
653 $aDiagnostic
653 $aProteína quimérica
653 $aTeste ELISA
700 1 $aRODRIGUES, R. de A.
700 1 $aTORRES, J. M.
700 1 $aMARCON, G. E. B.
700 1 $aFERREIRA, E. de C.
700 1 $aSOUZA, V. F. de
700 1 $aSARTI, E. F. B.
700 1 $aBERTOLLI, G. F.
700 1 $aARAUJO, D.
700 1 $aDEMARCHI, L. H. F.
700 1 $aLICHS, G.
700 1 $aZARDIN, M. U.
700 1 $aGONÇALVES, C. C. M.
700 1 $aCUENCA, V.
700 1 $aFAVACHO, A.
700 1 $aGUILHERMINO, J.
700 1 $aSANTOS, L. R. dos
700 1 $aARAUJO, F. R.
700 1 $aSILVA, M. R.
773 $tJournal of Immunological Methods$gv. 519, e113489, 2023.
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Embrapa Gado de Corte (CNPGC) |
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