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| Registros recuperados : 81 | |
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| 22. | | PÉRTILLE, F.; ZANELLA, R.; FELÍCIO, A. M.; LEDUR, M. C.; PEIXOTO, J. de O.; COUTINHO, L. L. Identification of polymorphisms associated with production traits on chicken (Gallus gallus) chromosome 4. Genetics and Molecular Research, v. 14, n. 3, p. 10717-10728, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 23. | | ZANELLA, R.; CANTAO, M. E.; LEDUR, M. C.; MORES, N.; ZANELLA, E. L.; OKINO, C. H.; ZANELLA, J. R. C. Whole genome sequencing on the identification of pathogens involved with high mortality in piglets. In: INTERNATIONAL PIG VETERINARY SOCIETY CONGRESS, 24., Dublin. Abstracts Book... Dublin, Ireland: IPVS, 2016. p. 460.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 24. | | FERREIRA, J. A.; FACCO, J. F.; RIZZETTI, T. M.; PRESTES, O. D.; ZANELLA, R.; TALAMINI, V.; FERREIRA, J. M. S.; BOTTOLI, C. B. G. Avaliação da água de coco e albúmen sólido na determinação de agrotóxicos por QuEChERS UHPLCMS/MS. In: SIMPÓSIO BRASILEIRO DE CROMATOGRAFIA E TÉCNICAS AFINS - SIMCRO, 2014, Campos do Jordão. Livro de resumos... Campos de Jordão, 2014.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 25. | | CANTAO, M. E.; ZANELLA, R.; PEIXOTO, J. de O.; IBELLI, A. M. G.; PANDOLFI, J. R. C.; SETTLES, M.; LEDUR, M. C. Age effects on gene expression related to the bacterial chondronecrosis with osteomyelitis in broiler. In: INTERNATIONAL PLANT & ANIMAL GENOME, 23., 2015, San Diego, CA. Abstracts? San Diego, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 26. | | CANTÃO, M. E.; OLIVEIRA FILHO, J. X. de; KICH, J. D.; KLEIN, C. S.; ZANELLA, R.; MORÉS, M. A. Z.; REBELATTO, R.; MORÉS, N. Comparative genomic analysis between pathogenic and non-pathogenic strains of pasteurella multocida. In: INTERNATIONAL PLANT & ANIMAL GENOME, 22., 2014, San Diego, CA. Abstracts... San Diego, 2014. P073.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 27. | | PETRY, B.; ZANELLA, R.; IBELLI, A. M. G.; MARCHESI, J. A. P.; PANDOLFI, J. R. C.; LEDUR, M. C.; PEIXOTO, J. de O. Comparação de métodos de homogeneização celular e congelamento para extração de RNA. In: JORNADA DE INICIAÇÃO CIENTÍFICA (JINC), 7., 2013, Concórdia. Anais... Brasília, DF: Embrapa, 2013. p. 13-14.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 28. | | SUPERTI, B. F. V.; SOUZA, A. P. de; MÜLLER, B. C.; SILVA, Z. da; ZANELLA, R.; ZANELLA, E. L.; MARQUES, M. G. Comparison of different surgical procedures to prepare teaser boars. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 31., 2017, Cabo de Santo Agostinho, PE. Proceedings... Belo Horizonte: Colégio Brasileiro de Reprodução Animal, 2017. p. 809.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 29. | | BATISTA, A. M.; ROSA, F.; FARIA, D. A.; DIAS, L. T.; WARPECHOWSKI, M. B.; LEDUR, M. C.; ZANELLA, R.; BRACCINI NETO, J. Diversidade genética de populações de porcos moura. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 6., 2020. Recursos genéticos e bioeconomia: inovação para um futuro sustentável: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2020. Trabalho 364. Evento online.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 30. | | SAVOLDI, I. R.; KOWACIC, R. da C.; IBELLI, A. M. G.; LOPES, L. dos S.; PALUDO, E.; ZANELLA, R.; PEIXOTO, J. de O.; LEDUR, M. C. Avaliação fenotípica do fêmur e da tíbia de frangos de corte afetados ou não com problemas locomotores. In: JORNADA DE INICIAÇÃO CIENTÍFICA (JINC), 9., 2015, Concórdia. Anais... Brasília: Embrapa, 2015. p. 104-105.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 31. | | SILVA, A. N. da; LAGO, L. V.; ZANELLA, E. L.; MARQUES, M. G.; PEIXOTO, J. de O.; SILVA, M. V. G. B.; LEDUR, M. C.; ZANELLA, R. Genome wide association test with scrotal hérnias in pigs. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 31., 2017, Cabo de Santo Agostinho, PE. Proceedings... Belo Horizonte: Colégio Brasileiro de Reprodução Animal, 2017. p. 845.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 32. | | ZANELLA, R.; PEIXOTO, J. de O.; CARDOSO, F. F.; CARDOSO, L. L.; BIEGELMEYER, P.; CANTAO, M. E.; OTAVIANO, A.; FREITAS, M. S.; CAETANO, A. R.; LEDUR, M. C. Genetic diversity analysis of two commercial breeds of pigs using genomic and pedigree data. Genetics Selection Evolution, v. 48, n. 24, 30 Mar. 2016.| Biblioteca(s): Embrapa Pecuária Sul; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Suínos e Aves. |
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| 33. | | PEIXOTO, J. de O.; KAWSKI, V. L.; IBELLI, A. M. G.; ZANELLA, R.; MAZZUCO, H.; SOUZA, C. G.; MUNARI, D. P.; JAENISCH, F. R. F.; LEDUR, M. C. Genetic evaluation of body weight and tibia resistance in broilers. In: WORLD CONGRESS OF GENETICS APPLIED TO LIVESTOCK PRODUCTION, 10., 2014, Vancouver. Proceedings... Vancouver: WCGALP: American Society of Animal Science, 2014.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 34. | | WOYANN, L. G.; ZDZIARSKI, A. D.; ZANELLA, R.; ROSA, A. C.; CASTRO, R. L. de; CAIERÃO, E.; TOIGO, M. D.; STORCK, L.; WU, J.; BENIN, G. Genetic gain over 30 years of spring wheat breeding in Brazil. Crop Science, v. 59, Sep./Oct. 2019.| Biblioteca(s): Embrapa Trigo. |
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| 35. | | ZANELLA, R.; GAVA, D.; PEIXOTO, J. de O.; SCHAEFER, R.; ZANELLA, J. R. C.; BIONDO, N.; SILVA, M. V. G. B.; CANTAO, M. E.; LEDUR, M. C. Genetic markers associated with influenza vaccination efficacy in swine. In: INTERNATIONAL SYMPOSIUM ON EMERGING AND RE-EMERGING PIG DISEASES, 7., 2015, Kyoto. Proceedings... Kyoto: Science Council of Japan; JSVS; JPVS; JASV, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 36. | | ZANELLA, R.; IBELLI, A. M. G.; PEIXOTO, J. de O.; CANTAO, M. E.; SILVA, M. V. G. B.; GIACHETTO, P. F.; FREITAS, M.; LOPES, J.; LEDUR, M. C. Genetic associations of farrowing length in two maternal lines of pigs. In: WORLD CONGRESS OF GENETICS APPLIED TO LIVESTOCK PRODUCTION, 10., 2014, Vancouver. Proceedings... Vancouver: WCGALP: American Society of Animal Science, 2014.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Suínos e Aves. |
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| 37. | | CAMARGO, J. de; MARQUES, M. G.; FACCIN, J. C. V.; JORGE NETO, P. N.; KICH, J. D.; SCARIOT, C. A.; ZANELLA, R. The feasibility of a swine semen storage system produced with bacteriostatic molecules to control bacterial proliferation. In: INTERNATIONAL SYMPOSIUM ON ANIMAL BIOLOGY OF REPRODUCTION, 9., 2022, Bento Gonçalves. Abstracts...Belo Horizonte: CBRA, 2022. Animal Reproduction, v. 19, n. 4, 2022.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 38. | | ROMERO TISOT, C. M.; ZANELLA, R.; SOUZA, A. P.; LÖF, L. M.; SOARES, J. C. M.; MARQUES, M. G.; ZANELLA, E. L. Melatonin effect on cryopreserved sperm cells of crioulo stallions. Open Access Journal of Veterinary Science & Research, v. 5, n. 2, 2020.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 39. | | TESSMANN, A. L.; MARCHESI, J. A. P.; ZANELLA, R.; CANTAO, M. E.; PANDOLFI, J. R. C.; IBELLI, A. M. G.; PEIXOTO, J. de O.; LEDUR, M. C. Mining for polymorphisms in the CALB gene and its associations with performance and carcass traits in a paternal broiler line. In: CONGRESSO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Ribeirão Preto: SBG, 2013. p. 149.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 40. | | FACIOLI, F. L.; BEZUTTI, G. da F.; BENDER, R. S.; MARQUES, M. G.; BONDAN, C.; ZANELLA, E. L; BERTOLINI, M.; ZANELLA, R. A rare case of heteropaternal twin calves after natural mating in Brazil. Animal Reproduction, v. 17, n. 4, e20200217, 2020.| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 81 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Suínos e Aves. |
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Data corrente: |
13/11/2018 |
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Data da última atualização: |
03/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
OKINO, C. H.; IBELLI, A. M. G.; BRENTANO, L.; ZANELLA, R.; MARQUES, M. G. |
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Afiliação: |
CINTIA HIROMI OKINO, CPPSE; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; LIANA BRENTANO, CNPSA; Ricardo Zanella, Universidade de Passo Fundo; MARIANA GROKE MARQUES, CNPSA. |
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Título: |
Evaluation of candidate reference genes for investigation of the uterine tissue and corpus luteum of pigs on day 6 after artificial insemination. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 198, p. 202-209, 2018. |
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DOI: |
10.1016/j.anireprosci.2018.09.020 |
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Idioma: |
Inglês |
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Conteúdo: |
Housekeeping genes (HKG) are genes necessary for the maintenance of basal cellular functions, regardless of the specific roles within a tissue. It, therefore, is expected that these genes will maintain a relatively constant expression profile when there are varying physiological conditions. The identification of tissue specific reference genes is highly important for the normalization of gene expression profiles among different tissues. In this sow study, the objective was to identify stable reference genes in the uterine tissue and corpus luteum (CL), 6 days post-artificial insemination. The stability of ubiquitin (UBB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), DNA topoisomerase 2-beta (TOP2B), histone H3 (H3F3A) and hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) abundances of mRNA were evaluated using the Bestkeeper technique. Briefly, total RNA was extracted for each tissue from 20 gilts (n=20), processed by RT-qPCR and submitted to analysis using the Bestkeeper technique, which allowed for the evaluation of the consistency in abundance of mRNA for the reference genes. For all evaluated genes, the abundance of mRNA was relatively consistent in the uterine tissue, with the greatest abundance being for the GAPDH and TOP2B genes. The analysis of these genes in the CL, however, indicated there was a relatively greater variation of mRNA abundance for the various reference genes. Data suggest that UBB was the reference gene with the most consistent relative abundance of mRNA and that this gene could be used as a reference for corpora lutea analyses of mRNA MenosHousekeeping genes (HKG) are genes necessary for the maintenance of basal cellular functions, regardless of the specific roles within a tissue. It, therefore, is expected that these genes will maintain a relatively constant expression profile when there are varying physiological conditions. The identification of tissue specific reference genes is highly important for the normalization of gene expression profiles among different tissues. In this sow study, the objective was to identify stable reference genes in the uterine tissue and corpus luteum (CL), 6 days post-artificial insemination. The stability of ubiquitin (UBB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), DNA topoisomerase 2-beta (TOP2B), histone H3 (H3F3A) and hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) abundances of mRNA were evaluated using the Bestkeeper technique. Briefly, total RNA was extracted for each tissue from 20 gilts (n=20), processed by RT-qPCR and submitted to analysis using the Bestkeeper technique, which allowed for the evaluation of the consistency in abundance of mRNA for the reference genes. For all evaluated genes, the abundance of mRNA was relatively consistent in the uterine tissue, with the greatest abundance being for the GAPDH and TOP2B genes. The analysis of these genes in the CL, however, indicated there was a relatively greater variation of mRNA abundance for the various reference genes. Data suggest that UBB was the reference gene with the most consistent relative a... Mostrar Tudo |
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Palavras-Chave: |
GAPDH; Housekeeping; TOP2B. |
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Thesagro: |
Inseminação; Reprodução Animal; Suíno; Útero. |
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Thesaurus NAL: |
Gene expression; Sows; Ubiquitin. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
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001 2099280
005 2019-01-03
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.anireprosci.2018.09.020$2DOI
100 1 $aOKINO, C. H.
245 $aEvaluation of candidate reference genes for investigation of the uterine tissue and corpus luteum of pigs on day 6 after artificial insemination.$h[electronic resource]
260 $c2018
520 $aHousekeeping genes (HKG) are genes necessary for the maintenance of basal cellular functions, regardless of the specific roles within a tissue. It, therefore, is expected that these genes will maintain a relatively constant expression profile when there are varying physiological conditions. The identification of tissue specific reference genes is highly important for the normalization of gene expression profiles among different tissues. In this sow study, the objective was to identify stable reference genes in the uterine tissue and corpus luteum (CL), 6 days post-artificial insemination. The stability of ubiquitin (UBB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), DNA topoisomerase 2-beta (TOP2B), histone H3 (H3F3A) and hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) abundances of mRNA were evaluated using the Bestkeeper technique. Briefly, total RNA was extracted for each tissue from 20 gilts (n=20), processed by RT-qPCR and submitted to analysis using the Bestkeeper technique, which allowed for the evaluation of the consistency in abundance of mRNA for the reference genes. For all evaluated genes, the abundance of mRNA was relatively consistent in the uterine tissue, with the greatest abundance being for the GAPDH and TOP2B genes. The analysis of these genes in the CL, however, indicated there was a relatively greater variation of mRNA abundance for the various reference genes. Data suggest that UBB was the reference gene with the most consistent relative abundance of mRNA and that this gene could be used as a reference for corpora lutea analyses of mRNA
650 $aGene expression
650 $aSows
650 $aUbiquitin
650 $aInseminação
650 $aReprodução Animal
650 $aSuíno
650 $aÚtero
653 $aGAPDH
653 $aHousekeeping
653 $aTOP2B
700 1 $aIBELLI, A. M. G.
700 1 $aBRENTANO, L.
700 1 $aZANELLA, R.
700 1 $aMARQUES, M. G.
773 $tAnimal Reproduction Science$gv. 198, p. 202-209, 2018.
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