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| Registros recuperados : 15 | |
| 2. |  | DALMOLIN, C. C.; VIVAN, A. L.; RIBEIRO, Z. M. A.; CASTRO, M. E. B. Análise de proteínas estruturais e de DNA de isolados temporais de Bombyx Mori Nucleopolyhedrovirus. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7., 2002, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2002. p. 32.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | DALMOLIN, C. C.; MAIA, E. A.; RIBEIRO, B. M.; SOUZA, M. L.; CASTRO, M. E. B. Clonagem do gene 25KFP de Anticarsia gemmatalis Mnpv para transformação estável de células de insetos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 115.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | DALMOLIN, C. C.; SANTOS, A. C. B.; RIBEIRO, Z. M. A.; CASTRO, M. E. B. Identificação do gene dnapol de Anticarsia gemmatalis Nucleopolyhedrovirus. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 7., 2002, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2002. p. 49.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | SANTOS, A. C. B.; RIBEIRO, Z. M. A.; DALMOLIN, C. C.; CASTRO, M. E. B. Infectividade do isolado 1-01 de bombyx mori nucleopolyhedrovirus em linhagens celulares de insetos. ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENETICOS E BIOTECNOLOGIA, 8., 2003, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2003. p. 77| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | PAIVA, M. R.; MIRANDA, H. A.; CIAMPI, A. Y.; DALMOLIN, C. C.; ALEGRIA, M. R. M.; BUSO, G. S. C. Busca de primers microssatélites, in silico, de genes-chave para características bióticas e abióticas de feijão, milho e mandioca. In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia, SP. A era da genômica: da bioestatística à bioinformática: anais. Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2005. p. 708.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | PAIVA, M. R.; MIRANDA, H. A.; CIAMPI, A. Y.; DALMOLIN, C. C.; ALEGRIA, M. R. M.; BUSO, G. S. C. Busca de primers microssatélites, in silico, de genes-chave para características bióticas e abióticas de feijão, milho e mandioca. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 156.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | RIBEIRO, Z. M. A.; MAIA, A. E.; DALMOLIN, C. C.; SOUZA, M. L.; RIBEIRO, B. M.; CASTRO, M. E. B. Construção de uma linhagem celular estável de Trichoplusia ni contendo o gene 25KFP do baculovírus AgMNPV. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 10., 2007, Brasília, DF. Inovar para preservar a vida. Resumos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 250).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | CASTRO, M. E. B.; DALMOLIN, C. C.; SANTOS, A. C. B. dos; RIBEIRO, Z. M. de A.; RIBEIRO, B. M.; SOUZA, M. L. Identification, location and partial sequencing of an Anticarsia gemmatalis nucleopolyhedrovirus DNA polymerase gene. In: INTERNATIONAL COLLOQUIUM ON INVERTEBRATE PATHOLOGY AND MICROBIAL CONTROL, 8.; INTERNATIONAL CONFERENCE ON BACILLUS THURINGIENSIS 6.; ANNUAL MEETING OF THE SIP, 35., 2002, Foz do Iguassu. Program and abstracts. Londrina: Embrapa Soja: Embrapa Recursos Genéticos e Biotecnologia: UEL: SIP, 2002. p. 84-85. (Embrapa Soja. Documentos, 183; Embrapa Recursos Genéticos e Biotecnologia, 73).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 15 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
23/04/2019 |
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Data da última atualização: |
06/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
DORTA, S. de O.; BALBINOTTE, J.; MONNERAT, R.; LOPES, J. R. S.; CUNHA, T. da; ZANARDI, O. Z.; MIRANDA, M. P. de; MACHADO, M. A.; ASTUA, J. de F. |
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Afiliação: |
SILVIA DE OLIVEIRA DORTA, ESALQ; JULIANA BALBINOTTE, Centro de Citricultura Sylvio Moreira; ROSE MONNERAT, ESALQ; JOAO ROBERTO SPOTTI LOPES, Centro de Citricultura Sylvio Moreira; TATIANE DA CUNHA, ESALQ; ODIMAR ZANUZO ZANARDI, Fundecitrus; MARCELO PEDREIRA DE MIRANDA, Fundecitrus; MARCOS ANTONIO MACHADO, Centro de Citricultura Sylvio Moreira; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Insect Science, p.1-12, 2019. |
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ISSN: |
1744-7917 |
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Idioma: |
Inglês |
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Conteúdo: |
Bacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus. MenosBacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Fruta Cítrica. |
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Thesaurus NAL: |
Citrus; Diaphorina citri; Plant diseases and disorders. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/196193/1/Dorta-et-al-2019-Insect-Science.pdf
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Marc: |
LEADER 02385naa a2200289 a 4500
001 2108421
005 2019-12-06
008 2019 bl uuuu u00u1 u #d
022 $a1744-7917
100 1 $aDORTA, S. de O.
245 $aSelection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera$bLiviidae) nymphs.$h[electronic resource]
260 $c2019
520 $aBacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus.
650 $aCitrus
650 $aDiaphorina citri
650 $aPlant diseases and disorders
650 $aDoença de Planta
650 $aFruta Cítrica
700 1 $aBALBINOTTE, J.
700 1 $aMONNERAT, R.
700 1 $aLOPES, J. R. S.
700 1 $aCUNHA, T. da
700 1 $aZANARDI, O. Z.
700 1 $aMIRANDA, M. P. de
700 1 $aMACHADO, M. A.
700 1 $aASTUA, J. de F.
773 $tInsect Science, p.1-12, 2019.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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