Data/hora: 12/06/2024 08:53:48

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	01/06/2023	
Data da última atualização: 	01/06/2023	
Autoria: 	DORTA, S. DE O.; ATTÍLIO, L. B.; ZANARDI, O. Z.; LOPES, J. R. S.; MACHADO, M. A.; ASTUA, J. de F.	
Afiliação: 	SÍLVIA DE OLIVEIRA DORTA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; LÍSIA BORGES ATTÍLIO, INSTITUTO AGRONÔMICO DE CAMPINAS; ODIMAR ZANUZO ZANARDI, INSTITUTO FEDERAL DE SANTA CATARINA; JOÃO ROBERTO SPOTTI LOPES, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; MARCOS ANTONIO MACHADO, INSTITUTO AGRONÔMICO DE CAMPINAS; JULIANA DE FREITAS ASTUA, CNPMF.	
Título: 	Genetic transformation of 'Hamlin' and 'Valencia' sweet orange plants expressing the cry11A gene of Bacillus thuringiensis as an additional tool for the management of Diaphorina citri (Hemiptera: Liviidae).	
Ano de publicação: 	2023	
Fonte/Imprenta: 	Journal of Biotechnolog, v.68, May, p.60-70, 2023.	
ISSN: 	0168-1656	
DOI: 	https://doi.org/10.1016/j.jbiotec.2023.04.007	
Idioma: 	Inglês	
Conteúdo: 	The Asian citrus psyllid (ACP) Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the vector of Candidatus Liberibacter spp., the bacteria associated with huanglongbing (HLB), the most devastating disease of citrus worldwide. HLB management has heavily relied on insecticide applications to control the ACP, although there are efforts towards more sustainable alternatives. In previous work, our group assessed the potential bioactivity of different strains of Bacillus thuringiensis (Eubacteriales: Bacillaceae) (Bt) containing cry/cyt genes as feasible tools to control ACP nymphs. Here, we report an attempt to use the cry11A gene from Bt to produce transgenic sweet orange plants using two promoters. For the genetic transformation, ?Hamlin? and ?Valencia? sweet orange seedlings were used as sources of explants. Transgenic plants were detected by polymerase chain reaction (PCR) with specific primers, and the transgene copy number was confirmed by Southern blot analyses. Transcript expression levels were determined by qPCR. Mortality assays of D. citri nymphs were carried out in a greenhouse, and the effect of the events tested ranged from 22% to 43% at the end of the five-day exposure period. To our knowledge, this is the first manuscript reporting the production of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs.	
Thesagro: 	Laranja; Melhoramento Vegetal.	
Thesaurus Nal: 	Oranges.	
Categoria do assunto: 	G Melhoramento Genético	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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