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| Registros recuperados : 44 | |
| 24. |  | BOITÉ, M. C.; CAMARGO, L. S. A.; GUIMARÃES, M. F. M.; SERAPIÃO, R. V.; WOHLRES-VIANA, S.; VIANA, J. H. M.; SÁ, W. F.; NOGUEIRA, L. A. G. Analysis of gene expression of in vitro produced bovine blastocyst cultured for 72 hours post-vitrification. In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 16., 2008, Budapest. Abstracts... Budapest, 2008. p. 186| Biblioteca(s): Embrapa Gado de Leite. |
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| 26. | | RODRIGUES, M. F.; ALVES, C. C. S.; FIGUEIREDO, B. B. M.; REZENDE, A. B.; WOHLRES-VIANA, S.; SILVA, V. L.; MACHADO, M. A.; TEIXEIRA, H. C. Tumour necrosis factor receptors and apoptosis of alveolar macrophages during early infection with attenuaed and virulent Mycobacterium bovis. Immunology, v. 139, n. 4, p. 503-512, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 27. | | ARASHIRO, E. K. N.; PALHÃO, M. P.; WOHLRES-VIANA, S.; SIQUEIRA, L. G. B.; CAMARGO, L. S. de A.; HENRY, M. R. J. M.; VIANA, J. H. M. In vivo collection of follicular fluid and granulosa cells from individual follicles of different diameters in cattle by an adapted ovum pick-up system. Reproductive Biology and Endocrinology, v. 11, article 73, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 28. | | CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M. Contrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos. Reproduction in Domestic Animals, v. 54, n. 10, p. 1357-1365, 2019.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 29. | | CAMARGO, L. S. de A.; PEREIRA, M. M.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; IGUMA, L. T.; CARVALHO, B. C. de; SIQUEIRA, L. G. B.; VIANA, J. H. M. Altered gene expression in bovine somatic cell nuclear-transfered embryos after trichostatin a treatment. Reproduction, Fertility and Development, v. 24, n. 1, p. 121-122, 2011.| Biblioteca(s): Embrapa Gado de Leite. |
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| 30. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; SUDRÉ, A. P.; SIQUEIRA, L. G. B.; PALHÃO, M. P.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; MACHADO, M. A.; VIANA, J. H. M. Luteinizing hormone receptor gene expression during follicular divergence in B. taurus vs B. indicus dairy breeds. Animal Reproduction, v.10, n.3, p. 425, Jul./Sept. 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 31. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; MINARE, T. P.; FERNANDES, C. A. C.; GRAZIA, J. G. V.; SIQUEIRA, L. G. B.; MACHADO, M. A.; VIANA, J. H. M. Differential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle. Theriogenology, v. 126, p. 68-74, 2019.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 32. | | PEREIRA, M. M.; WOHLRES-VIANA, S.; SALES, J. N. S.; CAMARGO, A. R.; QUINTAO, C. C. R.; RABELO, N. C.; CARVALHO, B. C. de; VIANA, J. H. M.; CAMARGO, L. S. de A.; GUIMARAES, M. F. M.; IGUMA, L. T. Differential gene expression od in vitro-matured bovine oocytes qith od without a polar body. In: ANNUAL CONFERENCE OF THE INTERNACIONAL EMBRYO TRANSFES SOCIETY, 2012, Phoenix. Proceedings... [S.l.:s.n.], 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 33. | | CAMARGO, L. S. de A.; BOITE, M. C.; WOHLRES-VIANA, S.; MOTA, G. B.; SERAPIÃO, R. V.; SÁ, W. F. de; VIANA, J. H. M.; NOGUEIRA, L. A. G. Osmotic challenge and expression of aquaporin 3 and Na/K ATPase genes in bovine embryos produced in vitro. Cryobiology, v. 63, n. 3, p. 256-262, 2011.| Biblioteca(s): Embrapa Gado de Leite. |
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| 34. | | PEREIRA, M. M.; BATISTA, R. I. T. P.; WOHLRES-VIANA, S.; BOITÉ, M. C.; SERAPIÃO, R. V.; POLISSENI, J.; IGUMA, L. T.; SÁ, W. F.; CAMARGO, L. S. A.; VIANA, J. H. M. Relação entre qualidade morfológica de complexos cumulus-oócitos bovinos e potencial de desenvolvimento in vitro. Acta Scientiae Veterinariae, v. 35, p. s1182, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.| Biblioteca(s): Embrapa Gado de Leite. |
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| 35. | | BATISTA, R. I. T. P.; FAZA, A. P.; WOHLRES-VIANA, S.; PINTO, I. S. B.; MORAES, M. N. de C. M.; LIMA, K. D. A.; CAMARGO, L. S. A.; VIANA, J. H. M. Resposta superovulatória em camundongos F1 produzidos a partir de diferentes estratégias de cruzamento entre linhagens endogâmicas. Acta Scientiae Veterinariae, v. 35, p. s1299, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.| Biblioteca(s): Embrapa Gado de Leite. |
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| 36. | | BOITÉ, M. C.; NOGUEIRA, L. A. G.; PEREIRA, M. M.; BATISTA, R. I. T. P.; WOHLRES-VIANA, S.; POLISSENI, J.; SERAPIÃO, R. V.; SÁ, W. F.; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. A. Comportamento osmótico de embriões bovinos cultivados em meio SOFaa ou CR2aa. Acta Scientiae Veterinariae, v. 35, p. s997, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.| Biblioteca(s): Embrapa Gado de Leite. |
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| 37. | | MINARÉ, T. P.; ARASHIRO, E. K. N.; WOHLRES-VIANA, S.; SIQUEIRA, L. G. B.; PALHÃO, M. P.; CORDEIRO, C. E. C.; FERNANDES, C. A. C.; VIANA, J. H. M. Characterization of individual differences in the efficiency of superovulation in the Gyr breed. Animal Reproduction, v.10, n.3, p. 546, Jul./Sept. 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 38. | | CAMPOS JÚNIOR, P. H. A.; CARVALHO, B. C.; SERAPIÃO, R. V.; PEREIRA, M. M.; COSTA, F. Q.; POLISSENI, J.; WOHLRES-VIANA, S.; SÁ, W. F.; VIANA, J. H. M.; CAMARGO, L. S. Produção e criotolerância de embriões bovinos produzidos sob diferentes sistemas de cultivo. In: 31ª SEMANA DE BIOLOGIA. MOSTRA DE PRODUÇÃO CIENTÍFICA. 14. 2008, Anais. Juiz de Fora. UFJF, 2008. 4 p. 1 CD.| Biblioteca(s): Embrapa Gado de Leite. |
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| 39. | | ARASHIRO, E. K. N.; WOHLRES-VIANA, S.; PALHÃO, M. P.; RABELO, N. A.; CAMARGO, L. S. de A.; SIQUEIRA, L. G. B.; PAIM, C. A. S.; VIANA, J. H. M. Follicular fluid and granulosa cell recovery from bovine follicles of different diameters using an adapted transvaginal-guided follicular aspiration system. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 38., 2012, Phoenix, Arizona. Abstracts... [S.l.: s.n.], 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 40. | | DOMINGUES, R.; WOHLRES-VIANA, S.; REIS, D. R. de L.; TEIXEIRA, H. C.; FERREIRA, A. P.; GUIMARÃES, S. E. F.; PRATA, M. C. de A.; FURLONG, J.; VERNEQUE, R. da S.; MACHADO, M. A. Expression of immune response genes in peripheral blood of cattle infested with Rhipicephalus microplus. Genetics and Molecular Research, v. 13, n. 2, p. 4013-21, 2014.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 44 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
14/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; REIS, D. R. de L.; FERNANDES, L. E.; PEIXOTO, M. G. C. D.; MACHADO, M. A.; VIANA, J. H. M. |
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Afiliação: |
S. Wohlres-Viana, UFJF; E. K. N. Arashiro, UFJF; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; L. E. Fernandes; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Polymorphisms and alternative splicing of the luteinizing hormone receptor of dairy cattle. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 15, n. 2, 2016. |
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Páginas: |
12 p. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The aim of this study was to screen for variability in the luteinizing hormone/choriogonadotropin receptor (LHCGR) and to determine the occurrence of LHCGR mRNA isoforms in two dairy breeds of cattle. Granulosa cells from dominant ovarian follicles were recovered from 16 Gir and 16 Holstein cows, and total RNA was extracted. Complementary DNA was synthesized and PCR was used to generate amplicons for sequencing. Chromatograms were evaluated, and multiple sequences were aligned and analyzed for the presence of polymorphisms, allele frequency, polymorphic information content (PIC), and Hardy-Weinberg equilibrium (HWE). Twentyone single nucleotide polymorphisms (SNPs) were identified in LH receptor mRNA. Seventeen SNPs were identified in Gir cattle (seven exclusively), and 14 were found in Holstein cattle (four exclusively). Seven of the 21 polymorphisms found did not alter which amino acid was translated. Eight SNPs caused a change to an amino acid in a different chemical group. Classification of SNPs according to PIC values identified 12 as being highly informative in Gir cattle and five in Holstein. Eight SNPs deviated from HWE in Gir compared with 11 in Holstein, and eight in both breeds. Two isoforms were also identified, one in exon 1, which lacks 30 nucleotides beginning at position 118, and the other in exon 10. Taken together, these data show that LHCGR in dairy cattle breeds has a high frequency of polymorphism and exists in multiple isoforms resulting from alternative splicing. MenosAbstract The aim of this study was to screen for variability in the luteinizing hormone/choriogonadotropin receptor (LHCGR) and to determine the occurrence of LHCGR mRNA isoforms in two dairy breeds of cattle. Granulosa cells from dominant ovarian follicles were recovered from 16 Gir and 16 Holstein cows, and total RNA was extracted. Complementary DNA was synthesized and PCR was used to generate amplicons for sequencing. Chromatograms were evaluated, and multiple sequences were aligned and analyzed for the presence of polymorphisms, allele frequency, polymorphic information content (PIC), and Hardy-Weinberg equilibrium (HWE). Twentyone single nucleotide polymorphisms (SNPs) were identified in LH receptor mRNA. Seventeen SNPs were identified in Gir cattle (seven exclusively), and 14 were found in Holstein cattle (four exclusively). Seven of the 21 polymorphisms found did not alter which amino acid was translated. Eight SNPs caused a change to an amino acid in a different chemical group. Classification of SNPs according to PIC values identified 12 as being highly informative in Gir cattle and five in Holstein. Eight SNPs deviated from HWE in Gir compared with 11 in Holstein, and eight in both breeds. Two isoforms were also identified, one in exon 1, which lacks 30 nucleotides beginning at position 118, and the other in exon 10. Taken together, these data show that LHCGR in dairy cattle breeds has a high frequency of polymorphism and exists in multiple isoforms resulting from a... Mostrar Tudo |
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Palavras-Chave: |
LHCGR isoforms. |
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Thesaurus NAL: |
reproduction; zebu. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/155636/1/Cnpgl-2016-GMR-Polylmorphisms.pdf
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Marc: |
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001 2064058
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100 1 $aWOHLRES-VIANA, S.
245 $aPolymorphisms and alternative splicing of the luteinizing hormone receptor of dairy cattle.$h[electronic resource]
260 $c2016
300 $a12 p.
520 $aAbstract The aim of this study was to screen for variability in the luteinizing hormone/choriogonadotropin receptor (LHCGR) and to determine the occurrence of LHCGR mRNA isoforms in two dairy breeds of cattle. Granulosa cells from dominant ovarian follicles were recovered from 16 Gir and 16 Holstein cows, and total RNA was extracted. Complementary DNA was synthesized and PCR was used to generate amplicons for sequencing. Chromatograms were evaluated, and multiple sequences were aligned and analyzed for the presence of polymorphisms, allele frequency, polymorphic information content (PIC), and Hardy-Weinberg equilibrium (HWE). Twentyone single nucleotide polymorphisms (SNPs) were identified in LH receptor mRNA. Seventeen SNPs were identified in Gir cattle (seven exclusively), and 14 were found in Holstein cattle (four exclusively). Seven of the 21 polymorphisms found did not alter which amino acid was translated. Eight SNPs caused a change to an amino acid in a different chemical group. Classification of SNPs according to PIC values identified 12 as being highly informative in Gir cattle and five in Holstein. Eight SNPs deviated from HWE in Gir compared with 11 in Holstein, and eight in both breeds. Two isoforms were also identified, one in exon 1, which lacks 30 nucleotides beginning at position 118, and the other in exon 10. Taken together, these data show that LHCGR in dairy cattle breeds has a high frequency of polymorphism and exists in multiple isoforms resulting from alternative splicing.
650 $areproduction
650 $azebu
653 $aLHCGR isoforms
700 1 $aARASHIRO, E. K. N.
700 1 $aREIS, D. R. de L.
700 1 $aFERNANDES, L. E.
700 1 $aPEIXOTO, M. G. C. D.
700 1 $aMACHADO, M. A.
700 1 $aVIANA, J. H. M.
773 $tGenetics and Molecular Research$gv. 15, n. 2, 2016.
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Embrapa Gado de Leite (CNPGL) |
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