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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
26/01/2016 |
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Data da última atualização: |
23/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
KHAJURIA, C.; VÉLEZ, A. M.; RANGASAMY, M.; WANG, H.; FISHILEVICH, E.; FREY, M. L. F.; CARNEIRO, N. P.; GANDRA, P.; NARVA, K. E.; SIEGFRIE, B. D. |
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Afiliação: |
NEWTON PORTILHO CARNEIRO, CNPMS. |
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Título: |
Parental RNA interference of genes involved in embryonic development of the western corn rootworm, Diabrotica virgifera virgifera LeConte. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Insect Biochemistry and Molecular Biology, Oxford, v. 63, p. 54-62, 2015. |
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DOI: |
10.1016/j.ibmb.2015.05.011 |
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Idioma: |
Inglês |
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Conteúdo: |
RNA interference (RNAi) is being developed as a potential tool for insect pest management and one of the most likely target pest species for transgenic plants that express double stranded RNA (dsRNA) is the western corn rootworm. Thus far, most genes proposed as targets for RNAi in rootworm cause lethality in the larval stage. In this study, we describe RNAi-mediated knockdown of two developmental genes, hunchback (hb) and brahma (brm), in the western corn rootworm delivered via dsRNA fed to adult fe- males. dsRNA feeding caused a significant decrease inhb and brm transcripts in the adult females. Although total oviposition was not significantly affected, there was almost complete absence of hatching in the eggs collected from females exposed to dsRNA for either gene. These results confirm that RNAi is systemic in nature for western corn rootworms. These results also indicate that hunchback and brahma play important roles in rootworm embryonic development and could provide useful RNAi targets in adult rootworms to prevent crop injury by impacting the population of larval progeny of exposed adults. The ability to deliver dsRNA in a trans-generational manner by feeding to adult rootworms may offer an additional approach to utilizing RNAi for rootworm pest management. The potential to develop parental RNAi technology targeting progeny of adult rootworms in combination with Bt proteins or dsRNA lethal to larvae may increase opportunities to develop sustainable approaches to rootworm management involving RNAi technologies for rootworm control. MenosRNA interference (RNAi) is being developed as a potential tool for insect pest management and one of the most likely target pest species for transgenic plants that express double stranded RNA (dsRNA) is the western corn rootworm. Thus far, most genes proposed as targets for RNAi in rootworm cause lethality in the larval stage. In this study, we describe RNAi-mediated knockdown of two developmental genes, hunchback (hb) and brahma (brm), in the western corn rootworm delivered via dsRNA fed to adult fe- males. dsRNA feeding caused a significant decrease inhb and brm transcripts in the adult females. Although total oviposition was not significantly affected, there was almost complete absence of hatching in the eggs collected from females exposed to dsRNA for either gene. These results confirm that RNAi is systemic in nature for western corn rootworms. These results also indicate that hunchback and brahma play important roles in rootworm embryonic development and could provide useful RNAi targets in adult rootworms to prevent crop injury by impacting the population of larval progeny of exposed adults. The ability to deliver dsRNA in a trans-generational manner by feeding to adult rootworms may offer an additional approach to utilizing RNAi for rootworm pest management. The potential to develop parental RNAi technology targeting progeny of adult rootworms in combination with Bt proteins or dsRNA lethal to larvae may increase opportunities to develop sustainable approaches to root... Mostrar Tudo |
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Palavras-Chave: |
Acido ribonucleico; Controle. |
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Thesagro: |
Besouro; Gene; Inseto. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02465naa a2200301 a 4500
001 2035201
005 2017-05-23
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1016/j.ibmb.2015.05.011$2DOI
100 1 $aKHAJURIA, C.
245 $aParental RNA interference of genes involved in embryonic development of the western corn rootworm, Diabrotica virgifera virgifera LeConte.$h[electronic resource]
260 $c2015
520 $aRNA interference (RNAi) is being developed as a potential tool for insect pest management and one of the most likely target pest species for transgenic plants that express double stranded RNA (dsRNA) is the western corn rootworm. Thus far, most genes proposed as targets for RNAi in rootworm cause lethality in the larval stage. In this study, we describe RNAi-mediated knockdown of two developmental genes, hunchback (hb) and brahma (brm), in the western corn rootworm delivered via dsRNA fed to adult fe- males. dsRNA feeding caused a significant decrease inhb and brm transcripts in the adult females. Although total oviposition was not significantly affected, there was almost complete absence of hatching in the eggs collected from females exposed to dsRNA for either gene. These results confirm that RNAi is systemic in nature for western corn rootworms. These results also indicate that hunchback and brahma play important roles in rootworm embryonic development and could provide useful RNAi targets in adult rootworms to prevent crop injury by impacting the population of larval progeny of exposed adults. The ability to deliver dsRNA in a trans-generational manner by feeding to adult rootworms may offer an additional approach to utilizing RNAi for rootworm pest management. The potential to develop parental RNAi technology targeting progeny of adult rootworms in combination with Bt proteins or dsRNA lethal to larvae may increase opportunities to develop sustainable approaches to rootworm management involving RNAi technologies for rootworm control.
650 $aBesouro
650 $aGene
650 $aInseto
653 $aAcido ribonucleico
653 $aControle
700 1 $aVÉLEZ, A. M.
700 1 $aRANGASAMY, M.
700 1 $aWANG, H.
700 1 $aFISHILEVICH, E.
700 1 $aFREY, M. L. F.
700 1 $aCARNEIRO, N. P.
700 1 $aGANDRA, P.
700 1 $aNARVA, K. E.
700 1 $aSIEGFRIE, B. D.
773 $tInsect Biochemistry and Molecular Biology, Oxford$gv. 63, p. 54-62, 2015.
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