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| 2. |  | MARTÍNEZ OCHOA, E. del C.; ANDRADE-RODRIGUEZ, M.; ROCANDIO RODRIGUEZ, M.; VILLEGAS MONTER, A. Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 11, p. 1629-1636, nov. 2012. Título em português: Identificação de plântulas zigóticas e nucelares em cultivares de manga poliembriônicas.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | ANDRADE-RODRÍGUEZ, M.; VILLEGAS-MONTER, A.; CARRILLO-CASTAÑEDA, G.; GARCÍA-VELÁZQUEZ, A. Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 6, p. 551-559, junho 2004 Título em português: Poliembrionia e identificação de seedlings zigóticos e nucelares de limão volkameriano com RAPD.| Biblioteca(s): Embrapa Unidades Centrais. |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
15/05/2013 |
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Data da última atualização: |
06/09/2017 |
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Autoria: |
MARTÍNEZ OCHOA, E. del C.; ANDRADE-RODRIGUEZ, M.; ROCANDIO RODRIGUEZ, M.; VILLEGAS MONTER, A. |
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Afiliação: |
ELISA DEL CARMEN MARTÍNEZ OCHOA, Colegio de Postgraduados; MARÍA ANDRADE-RODRIGUEZ, Universidad Autónoma del Estado de Morelos/Facultad de Ciencias Agropecuarias; MARIO ROCANDIO RODRIGUEZ, Colegio de Postgraduados; ANGEL VILLEGAS MONTER, Colegio de Postgraduados. |
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Título: |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 11, p. 1629-1636, nov. 2012. |
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ISSN: |
0100-204X |
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Idioma: |
Inglês |
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Notas: |
Título em português: Identificação de plântulas zigóticas e nucelares em cultivares de manga poliembriônicas. |
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Conteúdo: |
The objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in seven of nine 'Manila' polyembryonic seeds, and in four of seven 'Ataulfo' ones. Also, in polyembryonic seeds not all zygotic seedlings were produced by small embryos located at the micropyle. |
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Palavras-Chave: |
Funículo. |
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Thesagro: |
Cultura de embrião; Mangifera Indica; Marcador molecular; Polimorfismo. |
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Thesaurus NAL: |
Embryo culture; Genetic markers; Polymorphism. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/82846/1/IDENTIFICATION.pdf
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Marc: |
LEADER 01886naa a2200277 a 4500
001 1957984
005 2017-09-06
008 2012 bl uuuu u00u1 u #d
022 $a0100-204X
100 1 $aMARTÍNEZ OCHOA, E. del C.
245 $aIdentification of zygotic and nucellar seedlings in polyembryonic mango cultivars.
260 $c2012
500 $aTítulo em português: Identificação de plântulas zigóticas e nucelares em cultivares de manga poliembriônicas.
520 $aThe objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in seven of nine 'Manila' polyembryonic seeds, and in four of seven 'Ataulfo' ones. Also, in polyembryonic seeds not all zygotic seedlings were produced by small embryos located at the micropyle.
650 $aEmbryo culture
650 $aGenetic markers
650 $aPolymorphism
650 $aCultura de embrião
650 $aMangifera Indica
650 $aMarcador molecular
650 $aPolimorfismo
653 $aFunículo
700 1 $aANDRADE-RODRIGUEZ, M.
700 1 $aROCANDIO RODRIGUEZ, M.
700 1 $aVILLEGAS MONTER, A.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 47, n. 11, p. 1629-1636, nov. 2012.
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