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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
11/09/2018 |
Data da última atualização: |
31/10/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
KISHI, R. N. I.; STACH-MACHADO, D.; SINGULANI, J. de L.; SANTOS, C. T. dos; FUSCO-ALMEIDA, A. M.; CILLI, E. M.; ASTUA, J. de F.; PICCHI, S. C.; MACHADO, M. A. |
Afiliação: |
ROSANGELA NAOMI INUI KISHI, Instituto Agronomico; DAGMAR STACH-MACHADO, Universidade Estadual de Campinas; JUNYA DE LACORTE SINGULANI, Universidade Estadual Paulista Julio de Mesquita Filho; CLAUDIA TAVARES DOS SANTOS, Universidade Estadual Paulista Julio de Mesquita Filho; ANA MARISA FUSCO-ALMEIDA, Universidade Estadual Paulista Julio de Mesquita Filho; EDUARDO MAFFUD CILLI, Universidade Estadual Paulista Julio de Mesquita Fil; JULIANA DE FREITAS ASTUA, CNPMF; SIMONE CRISTINA PICCHI, Instituto Agronomico; MARCOS ANTONIO MACHADO, Instituto Agronômico. |
Título: |
Evaluation of cytotoxicity features of antimicrobial peptides with potential to control bacterial diseases of citrus. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Plos One, 2018. |
ISSN: |
1932-6203 |
DOI: |
10.1371/journal. pone.0203451 |
Idioma: |
Inglês |
Conteúdo: |
considered an alternative for pesticides used to control plant pathogens, including those affecting citrus. Brazil is the largest producer and exporter of frozen concentrated orange juice in the world. However, the citrus industry has been affected by several diseases such as citrus canker and huanglongbing (HLB), caused by the bacteria Xanthomonas citri subsp. citri (X.citri) and Candidatus Liberibacter asiaticus (CaLas), respectively. In order to control these pathogens, putative AMPs were prospected in databases containing citrus sequences. Furthermore, AMPs already reported in the literature were also used for in vitro and in vivo assays against X.citri. Since CaLas cannot be cultivated in vitro, surrogates as Sinorhizobium meliloti and Agrobacterium tumefaciens were used. This study reports the evaluation of six AMPs obtained from different sources, two of them from Citrus spp. (citrus-amp1 and citrus-amp2), three from amphibians (Hylin-a1, K0-W6-Hy-a1 and Ocellatin 4-analogue) and one from porcine (Tritrpticin). Peptides K0-W6-Hy-a1, Ocellatin 4-analogue, and citrus-amp1 showed bactericidal activity against X.citri and S. meliloti and bacteriostatic effect on A. tumefaciens. These results were confirmed for X.citri in planta. In addition cytotoxicity evaluations of these molecules were performed. The AMPs that showed the lowest hemolytic activities were Triptrpticin, citrus-amp1 and citrus-amp2. Citrus-amp1 and citrus-amp2 not presented toxicity in experiments using in vivo model, G. mellonella and U87 MG cells. To verify the interaction of these AMPs with bacteria and erythrocyte cell membranes, vesicles mimicking these cells were built. Citrus-amp1 and Tritrpticin exhibited higher affinity to bacterial membranes, while Ocellatin 4-analogue and Hylin-a1 showed higher affinity to erythrocyte membranes; exclude their use in citrus. This work demonstrates an essential alternative, trough AMPs obtained from Citrus spp., which can be feasibly used to control bacterial pathogens. Menosconsidered an alternative for pesticides used to control plant pathogens, including those affecting citrus. Brazil is the largest producer and exporter of frozen concentrated orange juice in the world. However, the citrus industry has been affected by several diseases such as citrus canker and huanglongbing (HLB), caused by the bacteria Xanthomonas citri subsp. citri (X.citri) and Candidatus Liberibacter asiaticus (CaLas), respectively. In order to control these pathogens, putative AMPs were prospected in databases containing citrus sequences. Furthermore, AMPs already reported in the literature were also used for in vitro and in vivo assays against X.citri. Since CaLas cannot be cultivated in vitro, surrogates as Sinorhizobium meliloti and Agrobacterium tumefaciens were used. This study reports the evaluation of six AMPs obtained from different sources, two of them from Citrus spp. (citrus-amp1 and citrus-amp2), three from amphibians (Hylin-a1, K0-W6-Hy-a1 and Ocellatin 4-analogue) and one from porcine (Tritrpticin). Peptides K0-W6-Hy-a1, Ocellatin 4-analogue, and citrus-amp1 showed bactericidal activity against X.citri and S. meliloti and bacteriostatic effect on A. tumefaciens. These results were confirmed for X.citri in planta. In addition cytotoxicity evaluations of these molecules were performed. The AMPs that showed the lowest hemolytic activities were Triptrpticin, citrus-amp1 and citrus-amp2. Citrus-amp1 and citrus-amp2 not presented toxicity in experiments using in... Mostrar Tudo |
Thesagro: |
Doença de Planta; Fruta Cítrica. |
Thesaurus Nal: |
Citrus; Plant diseases and disorders; Replant disease. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182724/1/Inui-Kishi-et-al-2018-journal.pone.0203451.pdf
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Marc: |
LEADER 02901naa a2200301 a 4500 001 2095442 005 2018-10-31 008 2018 bl uuuu u00u1 u #d 022 $a1932-6203 024 7 $a10.1371/journal. pone.0203451$2DOI 100 1 $aKISHI, R. N. I. 245 $aEvaluation of cytotoxicity features of antimicrobial peptides with potential to control bacterial diseases of citrus.$h[electronic resource] 260 $c2018 520 $aconsidered an alternative for pesticides used to control plant pathogens, including those affecting citrus. Brazil is the largest producer and exporter of frozen concentrated orange juice in the world. However, the citrus industry has been affected by several diseases such as citrus canker and huanglongbing (HLB), caused by the bacteria Xanthomonas citri subsp. citri (X.citri) and Candidatus Liberibacter asiaticus (CaLas), respectively. In order to control these pathogens, putative AMPs were prospected in databases containing citrus sequences. Furthermore, AMPs already reported in the literature were also used for in vitro and in vivo assays against X.citri. Since CaLas cannot be cultivated in vitro, surrogates as Sinorhizobium meliloti and Agrobacterium tumefaciens were used. This study reports the evaluation of six AMPs obtained from different sources, two of them from Citrus spp. (citrus-amp1 and citrus-amp2), three from amphibians (Hylin-a1, K0-W6-Hy-a1 and Ocellatin 4-analogue) and one from porcine (Tritrpticin). Peptides K0-W6-Hy-a1, Ocellatin 4-analogue, and citrus-amp1 showed bactericidal activity against X.citri and S. meliloti and bacteriostatic effect on A. tumefaciens. These results were confirmed for X.citri in planta. In addition cytotoxicity evaluations of these molecules were performed. The AMPs that showed the lowest hemolytic activities were Triptrpticin, citrus-amp1 and citrus-amp2. Citrus-amp1 and citrus-amp2 not presented toxicity in experiments using in vivo model, G. mellonella and U87 MG cells. To verify the interaction of these AMPs with bacteria and erythrocyte cell membranes, vesicles mimicking these cells were built. Citrus-amp1 and Tritrpticin exhibited higher affinity to bacterial membranes, while Ocellatin 4-analogue and Hylin-a1 showed higher affinity to erythrocyte membranes; exclude their use in citrus. This work demonstrates an essential alternative, trough AMPs obtained from Citrus spp., which can be feasibly used to control bacterial pathogens. 650 $aCitrus 650 $aPlant diseases and disorders 650 $aReplant disease 650 $aDoença de Planta 650 $aFruta Cítrica 700 1 $aSTACH-MACHADO, D. 700 1 $aSINGULANI, J. de L. 700 1 $aSANTOS, C. T. dos 700 1 $aFUSCO-ALMEIDA, A. M. 700 1 $aCILLI, E. M. 700 1 $aASTUA, J. de F. 700 1 $aPICCHI, S. C. 700 1 $aMACHADO, M. A. 773 $tPlos One, 2018.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
16/07/1996 |
Data da última atualização: |
18/03/2019 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
VIEIRA, V. J. de S.; RICHE, G. R.; TONNEAU, J. P. |
Afiliação: |
GILLES ROBERT RICHÉ, Consultor CPATSA; JEAN PHILIPPE TONNEAU, Consultor CIRAD. |
Título: |
Implantacao de pequena irrigacao com agua de pocos tubulares bombeados por catavento no tropico Semi-Árido - Massaroca, BA. |
Ano de publicação: |
1990 |
Fonte/Imprenta: |
In: SEMINARIO FRANCO BRASILEIRO DE PEQUENA IRRIGACAO, 1990, Recife, PE. Pesquisa e desenvolvimento: anais. Recife: SUDENE/Embaixada da Franca, 1990. |
Páginas: |
p. 113-130. |
Idioma: |
Português |
Conteúdo: |
Com o objetivo de avaliar a potencialidade das aguas de poco tubular para pequena irrigacao e tambem o desempenho de sistemas de bombeamento e distribuicao de agua por catavento ligado a um reservatorio para o consequente dimensionamento da area a ser irrigada, foi montado este trabalho de Pesquisa-Desenvolvimento nas comunidades de Lagoa do Angico e posteriormente, Canoa (Massaroca) tentando dar uma maior estabilidade aos sistemas de producoes do semi-arido nordestino. A presente proposta tenta desenvolver uma alternativa baseada sobre o aproveitamento das aguas subterraneas, mais permanente se o bombeamento foi limitado as possiblidades do lencol. E a comprovacao destas hipoteses que sera objetivo deste trabalho. |
Palavras-Chave: |
Bahia; Catavento; Fonte alternativa; Poco tubular; Small irrigation; Well; Windmill. |
Thesagro: |
Energia; Irrigação. |
Thesaurus NAL: |
energy; Irrigation; wind power. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/194392/1/Riche.pdf
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Marc: |
LEADER 01611nam a2200289 a 4500 001 1133671 005 2019-03-18 008 1990 bl uuuu u00u1 u #d 100 1 $aVIEIRA, V. J. de S. 245 $aImplantacao de pequena irrigacao com agua de pocos tubulares bombeados por catavento no tropico Semi-Árido - Massaroca, BA. 260 $aIn: SEMINARIO FRANCO BRASILEIRO DE PEQUENA IRRIGACAO, 1990, Recife, PE. Pesquisa e desenvolvimento: anais. Recife: SUDENE/Embaixada da Franca$c1990 300 $ap. 113-130. 520 $aCom o objetivo de avaliar a potencialidade das aguas de poco tubular para pequena irrigacao e tambem o desempenho de sistemas de bombeamento e distribuicao de agua por catavento ligado a um reservatorio para o consequente dimensionamento da area a ser irrigada, foi montado este trabalho de Pesquisa-Desenvolvimento nas comunidades de Lagoa do Angico e posteriormente, Canoa (Massaroca) tentando dar uma maior estabilidade aos sistemas de producoes do semi-arido nordestino. A presente proposta tenta desenvolver uma alternativa baseada sobre o aproveitamento das aguas subterraneas, mais permanente se o bombeamento foi limitado as possiblidades do lencol. E a comprovacao destas hipoteses que sera objetivo deste trabalho. 650 $aenergy 650 $aIrrigation 650 $awind power 650 $aEnergia 650 $aIrrigação 653 $aBahia 653 $aCatavento 653 $aFonte alternativa 653 $aPoco tubular 653 $aSmall irrigation 653 $aWell 653 $aWindmill 700 1 $aRICHE, G. R. 700 1 $aTONNEAU, J. P.
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