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| Registros recuperados : 35 | |
| 21. |  | VIEIRA, N. G.; ALVES, G. S. C.; FREIRE, L. P.; CARNEIRO, F. A.; MENDONÇA, L. M. C.; COSTA, T. S.; ALEKCEVETCH, J. C.; TAQUITA, J. A.; BLOCH JUNIOR, C.; MARRACCINI, P.; ANDRADE, A. C. No-hitomics: understanding the coffee unknowns. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 2012, San José. Proceedings... [S.l]: Association for Science and Information on Coffee (ASIC), 2012. p. 223.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 22. | | MARRACCINI, P.; ALVES, G. S. C.; VINECKY, F.; FREIRE, L. P.; VIEIRA, N. G.; RAMOS, H. J. O.; VIEIRA, L. G. E.; ELBELT, S.; MARQUES, T.; RODRIGUES, G. C.; ANDRADE, A. C. Identificação de genes com expressão diferencial em folhas de cafeeiro Coffea canephora e Coffea arabica submetidas à diferentes condições de estresse hídrico. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 6., 2009, Vitória. Inovação científica, competitividade e mudanças climáticas: anais. Vitória: Consórcio Pesquisa Café, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 23. | | MARRACCINI, P.; ALVES, G. S. C.; VINECKY, F.; FREIRE, L. P.; VIEIRA, N. G.; RAMOS, H. J. O.; VIEIRA, L. G. E.; ELBELT, S.; MARQUES, T.; RODRIGUES, G. C.; ANDRADE, A. C. Identificação de genes com expressão diferencial em folhas de cafeeiro Coffea canephora e Coffea arabica submetidas à diferentes condições de estresse hídrico. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 6., 2009, Vitória. Inovação científica, competitividade e mudanças climáticas: anais... Vitória: Consórcio Pesquisa Café, 2009.| Biblioteca(s): Embrapa Cerrados. |
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| 24. | | DUARTE, K. E.; VIEIRA, N. G.; MARTINS, P. K.; RIBEIRO, A. P.; CUNHA, B. A. B. D.; MOLINARI, H. B. C.; KOBAYASHI, A. K.; MARRACCINI, P.; ANDRADE, A. C. Genetic transformation of Setaria viridis with CcUNK8 for enhanced drought tolerance. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia, Colombia. [Proceedings...]. [Armenia]: ASIC, 2015. p. 42-45.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 25. | | DUARTE, K. E.; VIEIRA, N. G.; RÊGO, E. C. S.; MARTINS, P. K.; RIBEIRO, A. P.; CUNHA, B. A. D. B. da; MOLINARI, H. B. C.; KOBAYASHI, A. K.; SOUSA, C. A. de; MARRACCINI, P.; ANDRADE, A. C. Ensaio de déficit hidríco de Setaria viridis transformada com o gene órfão CcUNK8 de Coffea canephora. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 9., 2015, Curitiba. Anais... Brasília, DF: Embrapa Café, 2015.| Biblioteca(s): Embrapa Café. |
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| 26. | | VIEIRA, N. G.; FREIRE, L. P.; ALVES, G. S. C.; VINECKY, F.; ELBELT, S.; RAMOS, H. J. de O.; MONTAGNON, C.; VIEIRA, L. G. E.; LEROY, T.; POT, D.; SILVA, V. A.; RODRIGUES, G. C.; MARRACCINI, P.; ANDRADE, A. C. Expressão do gene RBCS1 em café: Coffea ortólogos, Coffea arabica homeólogos e variabilidade de expressão entre genótipos e sob estresse hídrico. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 27. | | COTTA, M. G.; BARROS, L. M. G.; ALMEIDA, J. D. de; LAMOTTE, F. de; BARBOSA, E. A.; VIEIRA, N. G.; ALVES, G. S. C.; VINECKY, F.; ANDRADE, A. C.; MARRACCINI, P. Lipid transfer proteins in coffee: isolation of Coffea orthologs, Coffea arabica homeologs, expression during coffee fruit development and promoter analysis in transgenic tobacco plants. Plant Molecular Biology, v. 85, n. 1, p.11-31, 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 28. | | HEIMBECK, I. G. R.; MELO, J. A. T.; PRATES, M. V.; VINECKY, F.; ALVES, G. S. C.; VIEIRA, N. G.; FREIRE, L. P.; BLOCH JUNIOR, C.; MARRACCINI, P.; ANDRADE, A. C. Desenvolvimento de novas metodologias para a identificação de proteínas durante o desenvolvimento de frutos do café (Coffea arabica L.). In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 29. | | HEIMBECK, I. G. R.; PRATES, M. V.; MELO, J. A. T.; VINECKY, F.; ALVES, G. S. C.; VIEIRA, N. G.; FREIRE, L. P.; BLOCH JUNIOR, C.; MARRACCINI, P.; ANDRADE, A. C. Desenvolvimento de uma metodologia para a identificação de metabólitos durante o desenvolvimento do frutos de café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 30. | | MARRACCINI, P.; FREIRE, L. P.; ALVES, G. S. C.; VIEIRA, N. G.; VINECKY, F.; ELBELT, S.; RAMOS, H. J; MONTAGNON, C.; VIEIRA, L. G. E.; LEROY, T.; POT, D.; SILVA, V. A.; RODRIGUES, G. C.; ANDRADE, A. C. RBCS1 expression in coffee: coffea orthologs, coffea arabica homeologs, and expression variability between genotypes and under drought stress BMC Plant Biology, v. 11, n. 85, 2011.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia. |
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| 31. | | MARRACCINI, P.; FREIRE, L. P.; ALVES, G. S. C.; VIEIRA, N. G.; VINECKY, F.; ELBELT, S.; RAMOS, H. J; MONTAGNON, C.; VIEIRA, L. G. E.; LEROY, T.; POT, D.; SILVA, V. A.; RODRIGUES, G. C.; ANDRADE, A. C. RBCS1 expression in coffee: coffea orthologs, coffea arabica homeologs, and expression variability between genotypes and under drought stress BMC Plant Biology, v. 11, n. 85, 2011.| Biblioteca(s): Embrapa Cerrados. |
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| 32. | | CARNEIRO, F. A.; MENDONÇA, L. M. C.; ALEKCEVETCH, J. C.; VIEIRA, N. G.; COSTA, T. S.; FERRAO, M. A. G.; SILVA, V. A.; MARRACCINI, P.; ANDRADE, A. C.; FERRAO, M. A. G. Rootomics: the first high-throughtput sequencing of cDNA extracted from roots of different clones of Coffea Canephora var. Conilon submitted to drought. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 2012, San José. Proceedings... [S.l]: Association for Science and Information on Coffee (ASIC), 2012. p. 223. P. 223| Biblioteca(s): Embrapa Café. |
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| 33. | | MARRACCINI, P.; VINECKY, F.; ALVES, G. S. C.; RAMOS, H. J. O.; ELBELT, S.; VIEIRA, N. G.; CARNEIRO, F. A.; SUJII, P. S.; ALEKCEVETCH, J. C.; SILVA, V. A.; DaMATTA, F. M.; FERRÃO, M. A. G.; LEROY, T.; POT, D.; VIEIRA, L. G. E.; SILVA, F. R. da; ANDRADE, A. C. Differentially expressed genes and proteins upon drought acclimation in tolerant and sensitive genotypes of Coffea canephora. Journal of Experimental Botany, Oxford, v. 63, n. 11, p. 4191-4212, 2012.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia. |
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| 34. | | MOFATTO, L. S.; CARNEIRO, F. de A.; VIEIRA, N. G.; DUARTE, K. E.; VIDAL, R. O.; ALEKCEVETCH, J. C.; COTTA, M. G.; VERDEIL, J-L.; LAPEYRE-MONTES, F.; LARTAUD, M.; LEROY, T.; DE BELLIS, F.; POT, D; RODRIGUES, G. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; ANDRADE, A. C.; MARRACCINI, P. Identification of candidate genes for drought tolerance in coffee by high-throughput sequencing in the shoot apex of different Coffea arabica cultivars. BMC Plant Biology, v. 16, n. 94, p. 1-18, 2016.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Café. |
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| 35. | | MARRACCINI, P.; VIEIRA, N. G.; DUARTE, K. E.; AQUINO, S. O.; CARNEIRO, F. A.; COSTA, T. S.; SUJII, P. S.; VINECKY, F.; ALEKCEVETCH, J. C.; ALVES, G. S. C.; LEROY, T.; BELLIS, F. de; FERRÃO, M. A. G.; DAMATTA, F. M.; POT, D.; SILVA, V. A.; RODRIGUES, G. C.; ANDRADE, A. C. Molecular responses of coffee plants to drought stress. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilheus. The challenge for the 21st century: book of abstracts. Ilhéus: International Advanced Biology Consortium, 2013. p. 29. Resumo S04T04.| Biblioteca(s): Embrapa Agricultura Digital. |
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| Registros recuperados : 35 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Café. |
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Data corrente: |
20/04/2016 |
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Data da última atualização: |
22/02/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MOFATTO, L. S.; CARNEIRO, F. de A.; VIEIRA, N. G.; DUARTE, K. E.; VIDAL, R. O.; ALEKCEVETCH, J. C.; COTTA, M. G.; VERDEIL, J-L.; LAPEYRE-MONTES, F.; LARTAUD, M.; LEROY, T.; DE BELLIS, F.; POT, D; RODRIGUES, G. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; ANDRADE, A. C.; MARRACCINI, P. |
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Afiliação: |
LUCIANA SOUTO MOFATTO, IB/Unicamp; FERNANDA DE ARAÚJO CARNEIRO, CENARGEN; NATALIA GOMES VIEIRA, CENARGEN; KAROLINE ESTEFANI DUARTE, CENARGEN; RAMON OLIVEIRA VIDAL, IB/Unicamp; JEAN CAROS ALEKCEVETCH, CENARGEN; MICHELLE GUITTON COTTA, CENARGEN; JEAN-LUC VERDEIL, CIRAD/UMR/AGAP; FABIENNE LEPEYRE-MONTES, CIRAD/UMR/AGAP; MARC LARTAUD, CIRAD/UMR/AGAP; THIERRY LEROY, CIRAD/UMR/AGAP; FABIEN DE BELLIS, CIRAD/UMR/AGAP; DAVID POT, CIRAD/UMR/AGAP; GUSTAVO COSTA RODRIGUES, CNPTIA; MARCELO FALSARELLA CARAZZOLLE, IB/Unicamp; GONÇALO AMARANTE GUIMARÃES PEREIRA, IB/Unicamp; ALAN CARVALHO ANDRADE, SAPC; PIERRE MARRACCINI, CENARGEN, CIRAD/UMR/AGAP. |
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Título: |
Identification of candidate genes for drought tolerance in coffee by high-throughput sequencing in the shoot apex of different Coffea arabica cultivars. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
BMC Plant Biology, v. 16, n. 94, p. 1-18, 2016. |
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DOI: |
10.1186/s12870-016-0777-5 |
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Idioma: |
Inglês |
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Conteúdo: |
BACKGROUND: Drought is a widespread limiting factor in coffee plants. It affects plant development, fruit production, bean development and consequently beverage quality. Genetic diversity for drought tolerance exists within the coffee genus. However, the molecular mechanisms underlying the adaptation of coffee plants to drought are largely unknown. In this study, we compared the molecular responses to drought in two commercial cultivars (IAPAR59, drought-tolerant and Rubi, drought-susceptible) of Coffea arabica grown in the field under control (irrigation) and drought conditions using the pyrosequencing of RNA extracted from shoot apices and analysing the expression of 38 candidate genes. RESULTS: Pyrosequencing from shoot apices generated a total of 34.7 Mbp and 535,544 reads enabling the identification of 43,087 clusters (41,512 contigs and 1,575 singletons). These data included 17,719 clusters (16,238 contigs and 1,575 singletons) exclusively from 454 sequencing reads, along with 25,368 hybrid clusters assembled with 454 sequences. The comparison of DNA libraries identified new candidate genes (n = 20) presenting differential expression between IAPAR59 and Rubi and/or drought conditions. Their expression was monitored in plagiotropic buds, together with those of other (n = 18) candidates genes. Under drought conditions, up-regulated expression was observed in IAPAR59 but not in Rubi for CaSTK1 (protein kinase), CaSAMT1 (SAM-dependent methyltransferase), CaSLP1 (plant development) and CaMAS1 (ABA biosynthesis). Interestingly, the expression of lipid-transfer protein (nsLTP) genes was also highly up-regulated under drought conditions in IAPAR59. This may have been related to the thicker cuticle observed on the abaxial leaf surface in IAPAR59 compared to Rubi. CONCLUSIONS: The full transcriptome assembly of C. arabica, followed by functional annotation, enabled us to identify differentially expressed genes related to drought conditions. Using these data, candidate genes were selected and their differential expression profiles were confirmed by qPCR experiments in plagiotropic buds of IAPAR59 and Rubi under drought conditions. As regards the genes up-regulated under drought conditions, specifically in the drought-tolerant IAPAR59, several corresponded to orphan genes but also to genes coding proteins involved in signal transduction pathways, as well as ABA and lipid metabolism, for example. The identification of these genes should help advance our understanding of the genetic determinism of drought tolerance in coffee. MenosBACKGROUND: Drought is a widespread limiting factor in coffee plants. It affects plant development, fruit production, bean development and consequently beverage quality. Genetic diversity for drought tolerance exists within the coffee genus. However, the molecular mechanisms underlying the adaptation of coffee plants to drought are largely unknown. In this study, we compared the molecular responses to drought in two commercial cultivars (IAPAR59, drought-tolerant and Rubi, drought-susceptible) of Coffea arabica grown in the field under control (irrigation) and drought conditions using the pyrosequencing of RNA extracted from shoot apices and analysing the expression of 38 candidate genes. RESULTS: Pyrosequencing from shoot apices generated a total of 34.7 Mbp and 535,544 reads enabling the identification of 43,087 clusters (41,512 contigs and 1,575 singletons). These data included 17,719 clusters (16,238 contigs and 1,575 singletons) exclusively from 454 sequencing reads, along with 25,368 hybrid clusters assembled with 454 sequences. The comparison of DNA libraries identified new candidate genes (n = 20) presenting differential expression between IAPAR59 and Rubi and/or drought conditions. Their expression was monitored in plagiotropic buds, together with those of other (n = 18) candidates genes. Under drought conditions, up-regulated expression was observed in IAPAR59 but not in Rubi for CaSTK1 (protein kinase), CaSAMT1 (SAM-dependent methyltransferase), CaSLP1 (plant deve... Mostrar Tudo |
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Palavras-Chave: |
Candidate gene; Coffee; Differential gene expression; Real-time PCR (RT-qPCR); RNA-Seq. |
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Thesagro: |
Café; Coffea Arábica; Resistência à seca; Seca. |
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Thesaurus NAL: |
Drought; Drought tolerance; Sequence analysis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142466/1/Identification-of-candidate-genes.pdf
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Marc: |
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024 7 $a10.1186/s12870-016-0777-5$2DOI
100 1 $aMOFATTO, L. S.
245 $aIdentification of candidate genes for drought tolerance in coffee by high-throughput sequencing in the shoot apex of different Coffea arabica cultivars.$h[electronic resource]
260 $c2016
520 $aBACKGROUND: Drought is a widespread limiting factor in coffee plants. It affects plant development, fruit production, bean development and consequently beverage quality. Genetic diversity for drought tolerance exists within the coffee genus. However, the molecular mechanisms underlying the adaptation of coffee plants to drought are largely unknown. In this study, we compared the molecular responses to drought in two commercial cultivars (IAPAR59, drought-tolerant and Rubi, drought-susceptible) of Coffea arabica grown in the field under control (irrigation) and drought conditions using the pyrosequencing of RNA extracted from shoot apices and analysing the expression of 38 candidate genes. RESULTS: Pyrosequencing from shoot apices generated a total of 34.7 Mbp and 535,544 reads enabling the identification of 43,087 clusters (41,512 contigs and 1,575 singletons). These data included 17,719 clusters (16,238 contigs and 1,575 singletons) exclusively from 454 sequencing reads, along with 25,368 hybrid clusters assembled with 454 sequences. The comparison of DNA libraries identified new candidate genes (n = 20) presenting differential expression between IAPAR59 and Rubi and/or drought conditions. Their expression was monitored in plagiotropic buds, together with those of other (n = 18) candidates genes. Under drought conditions, up-regulated expression was observed in IAPAR59 but not in Rubi for CaSTK1 (protein kinase), CaSAMT1 (SAM-dependent methyltransferase), CaSLP1 (plant development) and CaMAS1 (ABA biosynthesis). Interestingly, the expression of lipid-transfer protein (nsLTP) genes was also highly up-regulated under drought conditions in IAPAR59. This may have been related to the thicker cuticle observed on the abaxial leaf surface in IAPAR59 compared to Rubi. CONCLUSIONS: The full transcriptome assembly of C. arabica, followed by functional annotation, enabled us to identify differentially expressed genes related to drought conditions. Using these data, candidate genes were selected and their differential expression profiles were confirmed by qPCR experiments in plagiotropic buds of IAPAR59 and Rubi under drought conditions. As regards the genes up-regulated under drought conditions, specifically in the drought-tolerant IAPAR59, several corresponded to orphan genes but also to genes coding proteins involved in signal transduction pathways, as well as ABA and lipid metabolism, for example. The identification of these genes should help advance our understanding of the genetic determinism of drought tolerance in coffee.
650 $aDrought
650 $aDrought tolerance
650 $aSequence analysis
650 $aCafé
650 $aCoffea Arábica
650 $aResistência à seca
650 $aSeca
653 $aCandidate gene
653 $aCoffee
653 $aDifferential gene expression
653 $aReal-time PCR (RT-qPCR)
653 $aRNA-Seq
700 1 $aCARNEIRO, F. de A.
700 1 $aVIEIRA, N. G.
700 1 $aDUARTE, K. E.
700 1 $aVIDAL, R. O.
700 1 $aALEKCEVETCH, J. C.
700 1 $aCOTTA, M. G.
700 1 $aVERDEIL, J-L.
700 1 $aLAPEYRE-MONTES, F.
700 1 $aLARTAUD, M.
700 1 $aLEROY, T.
700 1 $aDE BELLIS, F.
700 1 $aPOT, D
700 1 $aRODRIGUES, G. C.
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700 1 $aPEREIRA, G. A. G.
700 1 $aANDRADE, A. C.
700 1 $aMARRACCINI, P.
773 $tBMC Plant Biology$gv. 16, n. 94, p. 1-18, 2016.
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Embrapa Agricultura Digital (CNPTIA) |
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