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| Registros recuperados : 54 | |
| 42. |  | SAMPAIO, E. dos S.; VIEIRA, L. de J.; CONCEIÇÃO, J. Q. da; SANTOS, L. B. P. R. dos; LEDO, C. A. da S.; SOUZA, F. V. D. Enraizamento de estacas de espécies silvetres e hibrido de Manihot. In: CONGRESSO BRASILEIRO DE MANDIOCA, 16.; CONGRESSO LATINO-AMERICANO E CARIBENHO DE MANDIOCA, 2015, Foz do Iguaçu. Integração: segurança alimentar e geração de renda: anais. Foz do Iguaçu: SBM, 2015. 1 CD-ROM. 4p.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 43. | | PASSOS, R. R.; VIEIRA, L. de J.; OLIVEIRA, T. da S.; SANTANA, J. R. F.; LEDO, C. A. da S.; ALVES, A. A. C.; SOUZA, F. V. D. Desidratação de grãos de pólen de diferentes subespécies de M. esculenta. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 44. | | VIEIRA, L. de J.; SANTANA, J. R. F. de; SILVEIRA, T. C. da; ALVES, A. A. C.; SOUZA, F. V. D. Conservação e longevidade de pólen de acessos de Manihot esculenta. In: CONGRESSO BRASILEIRO DE MANDIOCA, 14.; FEIRA BRASILEIRA DA MANDIOCA, 1., 2011, Maceió. Mandioca: fonte de alimento e energia: anais. Maceió: ABAM: SBM, 2011. 1 CD ROM. Melhoramento genético. Resumo n. 266.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 45. | | VIEIRA, L. de J.; ALVES, A. A. C.; VILARINHOS, A. D.; DALTRO, P. S.; SOUZA, W. F.; SILVA, P. H. da; SANTOS, V. J. dos. Seleção de híbridos de mandioca para resistência a vírus de mosaico africano assistida por marcadores moleculares. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA TROPICAL, 2., 2008, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2008. RESUMO_0733.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 46. | | VIEIRA, L. de J.; SANTANA, J. R. F. de; ALVES, A. A. C.; LEDO, C. A. da S.; SOUZA, F. V. D. Use of aniline blue stain to observing pollen tubes development in different Manihot Mill. species. African Journal of Agricultural Research, v. 10, n. 15, p. 1805-1809, 9 April, 2015.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 47. | | SILVEIRA, T. C. da; VIEIRA, L. de J.; SOUZA, F. V. D.; LEDO, C. A. da S.; ALVES, A. A. C. Viabilidade de pólen de sub-espécies de Manihot esculenta. In: CONGRESSO BRASILEIRO DE MANDIOCA, 14.; FEIRA BRASILEIRA DA MANDIOCA, 1., 2011, Maceió. Mandioca: fonte de alimento e energia: anais. Maceió: ABAM: SBM, 2011. 1 CD ROM. Propagação. Resumo n. 249.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 48. | | VIEIRA, L. de J.; SOARES, T. L.; ROSSI, M. L.; ALVES, A. A. C.; SANTOS, F. de A. R. dos; SOUZA, F. V. D. Viability, production and morphology of pollen grains for diff erent species in the genus Manihot (Euphorbiaceae). Acta Botanica Brasilica, v. 26, n. 2, p. 350-356, 2012.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 49. | | VIEIRA, L. de J.; PASSOS, R. R.; OLIVIERA, T. da S.; SANTANA, J. R. F. de; LEDO, C. A. da S.; ALVES, A. A. C.; SOUZA, F. V. D. Avaliação da longevidade de grãos de pólen de de Manihot esculenta spp. flabellifolia por teste colorimétrico e fluorescência. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 50. | | VIEIRA, L. de J.; OLIVEIRA, T. da S.; PASSOS, R. R.; SANTANA, J. R. F. de; LEDO, C. A. da S.; ALVES, A. A. C.; SOUZA, F. V. D. Avaliação de métodos para desidratação de grãos de pólen de M. esculenta. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 51. | | OLIVEIRA, T. da S.; VIEIRA, L. de J.; PASSOS, R. R.; SANTANA, J. R. F. de; MARTINS, M. L. L.; LEDO, C. A. da S.; ALVES, A. A. C.; SOUZA, F. V. D. Avaliação da receptividade de estigmas de Manihot esculenta spp. flabellifolia. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 52. | | SOUZA, F. V. D.; KAYA, E.; VIEIRA, L. de J.; SOUZA, E. H. de; AMORIM, V. B. de O.; SKOGERBOE, D.; MATSUMOTO, T.; ALVES, A. A. C.; LEDO, C. A. da S.; JENDEREK, M. M. Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes. Plant Cell, Tissue and Organ Culture, v.123, n.2, nov. 2015.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 53. | | VIEIRA, L. de J.; SANTANA, J. R. F. de; VIDAL, A. M.; ALVES, A. A. C.; SOUZA, A. da S.; LEDO, C. A. da S.; SOUZA, F. V. D. Use of multivariate analysis to evaluate the effect of sucrose on in vitro cassava conservation. African Journal of Biotechnology, February, v.14, n.5, p. 419-424, 2015.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 54. | | SOUZA, F. V. D.; KAYA, E.; VIEIRA, L. de J.; SOUZA, A. da S.; CARVALHO, M. de J. da S.; SANTOS, E. B.; ALVES, A. A. C.; ELLIS, D. Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique. Scientia Horticulturae, v.224, p.302-305, October ,2017.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 54 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
13/11/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOUZA, F. V. D.; KAYA, E.; VIEIRA, L. de J.; SOUZA, E. H. de; AMORIM, V. B. de O.; SKOGERBOE, D.; MATSUMOTO, T.; ALVES, A. A. C.; LEDO, C. A. da S.; JENDEREK, M. M. |
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Afiliação: |
FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ERGUN KAYA, National Center for Genetic Resources Preservation; LÍVIA DE JESUS VIEIRA; EVERTON HILO DE SOUZA; VANUSIA BATISTA DE OLIVEIRA AMORIM; DIANNE SKOGERBOE, National Center for Genetic Resources Preservation; TRACIE MATSUMOTO, Pacific Basin Agriculture Research Center; ALFREDO AUGUSTO CUNHA ALVES, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; MARIA M. JENDEREK, National Center for Genetic Resources Preservation. |
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Título: |
Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v.123, n.2, nov. 2015. |
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ISSN: |
0167-6857 |
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DOI: |
10.1007/s11240-015-0899-8 |
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Idioma: |
Português |
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Conteúdo: |
Germplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the first report on histological changes occurring in cryopreserved Ananas tissue. MenosGermplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the ... Mostrar Tudo |
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Thesagro: |
Abacaxi; Ananás Comosus. |
|
Thesaurus NAL: |
Histology; Pineapples. |
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Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
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100 1 $aSOUZA, F. V. D.
245 $aDroplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes.$h[electronic resource]
260 $c2015
520 $aGermplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the first report on histological changes occurring in cryopreserved Ananas tissue.
650 $aHistology
650 $aPineapples
650 $aAbacaxi
650 $aAnanás Comosus
700 1 $aKAYA, E.
700 1 $aVIEIRA, L. de J.
700 1 $aSOUZA, E. H. de
700 1 $aAMORIM, V. B. de O.
700 1 $aSKOGERBOE, D.
700 1 $aMATSUMOTO, T.
700 1 $aALVES, A. A. C.
700 1 $aLEDO, C. A. da S.
700 1 $aJENDEREK, M. M.
773 $tPlant Cell, Tissue and Organ Culture$gv.123, n.2, nov. 2015.
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