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| 5. |  | VIEIRA, L. de J.; SOUZA, F. V. D.; ALVES, A. A. C.; VIDAL, M. das G. Produção, tamanho e viabilidade de pólen de variedades domésticas e espécies silvestres de mandioca. In: SEMINÁRIO DE PESQUISA DO RECÔNCAVO DA BAHIA, 3.; SEMINÁRIO ESTUDANTIL DE PESQUISA DA UFRB, 3.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA UFRB, 3., 2009, Cruz das Almas. Evolução do conhecimento e o desenvolvimento social: [resumos...]. Cruz das Almas: Universidade Federal do Recôncavo da Bahia, 2009. 01 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 17. | | SOARES, T. L.; SOUZA, F. V. D.; SOUZA, E. H. de; VIEIRA, L. de J. Viabilidade de pólen de abacaxizeiro Ananas comosus var. comosus. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 400.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 19. | | VIEIRA, L. de J.; SOUZA, F. V. D.; ALVES, A. A. C. Viabilidade de pólen de mandioca silvestre sob diferentes condições de armazenamento. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. 1 CD-ROM (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304). pdf 485.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 20. | | VIEIRA, L. de J.; SOUZA, F. V. D.; SOARES, T. L.; VIDAL, M. das G.; ALVES, A. A. C. Produção e tamanho de pólen de variedades domésticas e espécies silvestres de mandioca. Revista Raízes e Amidos Tropicais, Botucatu, v. 5, p. 727-730, jul. 2009. 1 CD-ROM. Edição dos Anais do XIII Congresso Brasileiro de Mandioca; VII Workshop sobre Tecnologia em Agroindústrias de Tuberosas Tropicais. Botucatu, jul. 2009.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
13/11/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SOUZA, F. V. D.; KAYA, E.; VIEIRA, L. de J.; SOUZA, E. H. de; AMORIM, V. B. de O.; SKOGERBOE, D.; MATSUMOTO, T.; ALVES, A. A. C.; LEDO, C. A. da S.; JENDEREK, M. M. |
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Afiliação: |
FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ERGUN KAYA, National Center for Genetic Resources Preservation; LÍVIA DE JESUS VIEIRA; EVERTON HILO DE SOUZA; VANUSIA BATISTA DE OLIVEIRA AMORIM; DIANNE SKOGERBOE, National Center for Genetic Resources Preservation; TRACIE MATSUMOTO, Pacific Basin Agriculture Research Center; ALFREDO AUGUSTO CUNHA ALVES, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; MARIA M. JENDEREK, National Center for Genetic Resources Preservation. |
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Título: |
Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v.123, n.2, nov. 2015. |
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ISSN: |
0167-6857 |
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DOI: |
10.1007/s11240-015-0899-8 |
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Idioma: |
Português |
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Conteúdo: |
Germplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the first report on histological changes occurring in cryopreserved Ananas tissue. MenosGermplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the ... Mostrar Tudo |
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Thesagro: |
Abacaxi; Ananás Comosus. |
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Thesaurus NAL: |
Histology; Pineapples. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
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024 7 $a10.1007/s11240-015-0899-8$2DOI
100 1 $aSOUZA, F. V. D.
245 $aDroplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes.$h[electronic resource]
260 $c2015
520 $aGermplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the first report on histological changes occurring in cryopreserved Ananas tissue.
650 $aHistology
650 $aPineapples
650 $aAbacaxi
650 $aAnanás Comosus
700 1 $aKAYA, E.
700 1 $aVIEIRA, L. de J.
700 1 $aSOUZA, E. H. de
700 1 $aAMORIM, V. B. de O.
700 1 $aSKOGERBOE, D.
700 1 $aMATSUMOTO, T.
700 1 $aALVES, A. A. C.
700 1 $aLEDO, C. A. da S.
700 1 $aJENDEREK, M. M.
773 $tPlant Cell, Tissue and Organ Culture$gv.123, n.2, nov. 2015.
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