Data/hora: 11/06/2024 23:13:50

Biblioteca(s): | Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 12/12/2018
Data da última atualização: | 24/01/2023
Autoria: | WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; MINARE, T. P.; FERNANDES, C. A. C.; GRAZIA, J. G. V.; SIQUEIRA, L. G. B.; MACHADO, M. A.; VIANA, J. H. M.
Afiliação: | S. WOHLRES-VIANA, UFJF; E. K. N. ARASHIRO, UFF; T. P. MINARE, Universidade José do Rosario Vellano, Alfenas, MG; C. A. C. FERNANDES, Universidade Jose do Rosario Vellano / Biotran Biotecnologia Animal, Alfenas, MG; J. G. V. GRAZIA, UFMG; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen.
Título: | Differential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle.
Ano de publicação: | 2019
Fonte/Imprenta: | Theriogenology, v. 126, p. 68-74, 2019.
DOI: | 10.1016/j.theriogenology.2018.12.004
Idioma: | Inglês
Conteúdo: | Abstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3&#8239;±&#8239;1.2 ova/embryos per flush, n&#8239;=&#8239;5) or poor responders (1.1&#8239;±&#8239;0.3 ova/embryos per flush, n&#8239;=&#8239;5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8&#8239;mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype.
Palavras-Chave: | In vivo embryo production.
Thesaurus Nal: | Embryo transfer; Luteinizing hormone.

Categoria do assunto:

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L Ciência Animal e Produtos de Origem Animal

Registro original: | Embrapa Gado de Leite (CNPGL)

Exemplares:

CENARGEN - (37494-1) - UPC - AP - DD -  -  -  - DIS
CNPGL - (24514-1) - UPC - AP - DD -  -  -  - DIS