Data/hora: 02/06/2024 15:54:51

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	18/12/2018	
Data da última atualização: 	24/01/2023	
Autoria: 	OTTO, P. I.; GUIMARÃES, S. E. F.; VERARDO, L. L.; AZEVEDO, A. L. S.; VANDENPLAS, J.; SOARES, A. C. C.; SEVILLANO, C. A.; VERONEZE R; PIRES, M. de F. A.; FREITAS, C. de; PRATA, M. C. de A.; FURLONG, J.; VERNEQUE, R. da S.; MARTINS, M. F.; PANETTO, J. C. do C.; CARVALHO, W. A.; GOBO, D. O. R.; SILVA, M. V. G. B.; MACHADO, M. A.	
Afiliação: 	PAMELA I. OTTO, UFV; SIMONE ELIZA FACIONI GUIMARAES, UFV; LUCAS L. VERARDO, UFV; ANA LUISA SOUSA AZEVEDO, CNPGL; J. VANDENPLAS, Wageningen University; ALINE C. C. SOARES, UFV; CLAUDIA A. SEVILLANO, Wageningen University; Topigs Norsvin Research Center, Beuningen; RENATA VERONEZE, UFV; MARIA DE FATIMA AVILA PIRES, CNPGL; CELIO DE FREITAS, CNPGL; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; JOHN FURLONG, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; MARTA FONSECA MARTINS, CNPGL; JOAO CLAUDIO DO CARMO PANETTO, CNPGL; WANESSA ARAUJO CARVALHO, CNPGL; DIEGO O. R. GOBO, UFV; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; MARCO ANTONIO MACHADO, CNPGL.	
Título: 	Genome-wide association studies for tick resistance in Bos taurus × Bos indicus crossbred cattle: A deeper look into this intricate mechanism.	
Ano de publicação: 	2018	
Fonte/Imprenta: 	Journal of Dairy Science, v. 101, n. 12, p. 11020-11032, 2018.	
DOI: 	10.3168/jds.2017-14223	
Idioma: 	Inglês	
Conteúdo: 	ABSTRACT Rhipicephalus (Boophilus) microplus is the main cattle ectoparasite in tropical areas. Gir × Holstein crossbred cows are well adapted to different production systems in Brazil. In this context, we performed genome-wide association study (GWAS) and post-GWAS analyses for R. microplus resistance in an experimental Gir × Holstein F2 population. Single nucleotide polymorphisms (SNP) identified in GWAS were used to build gene networks and to investigate the breed of origin for its alleles. Tick artificial infestations were performed during the dry and rainy seasons. Illumina BovineSNP50 BeadChip (Illumina Inc., San Diego, CA) and single-step BLUP procedure was used for GWAS. Post-GWAS analyses were performed by gene ontology terms enrichment and gene transcription factors networks, generated from enriched transcription factors, identified from the promoter sequences of selected gene sets. The genetic origin of marker alleles in the F2 population was assigned using the breed of origin of alleles approach. Heritability estimates for tick counts were 0.40 ± 0.11 in the rainy season and 0.54 ± 0.11 in the dry season. The top ten 0.5-Mbp windows with the highest percentage of genetic variance explained by SNP markers were found in chromosomes 10 and 23 for both the dry and rainy seasons. Gene network analyses allowed the identification of genes involved with biological processes relevant to immune system functions (TREM1, TREM2, and CD83). Gene-transcription factors network allowed the identification of genes involved with immune functions (MYO5A, TREML1, and PRSS16). In resistant animals, the average proportion of animals showing significant SNPs with paternal and maternal alleles originated from Gir breed was 44.8% whereas the proportion of animals with both paternal and maternal alleles originated from Holstein breed was 11.3%. Susceptible animals showing both paternal and maternal alleles originated from Holstein breed represented 44.6% on average, whereas both paternal and maternal alleles originated from Gir breed animals represented 9.3%. This study allowed us to identify candidate genes for tick resistance in Gir × Holstein crossbreds in both rainy and dry seasons. According to the origin of alleles analysis, we found that most animals classified as resistant showed 2 alleles from Gir breed, while the susceptible ones showed alleles from Holstein. Based on these results, the identified genes may be thoroughly investigated in additional experiments aiming to validate their effects on tick resistance phenotype in cattle.	
Palavras-Chave: 	Breed of origin; Gene network; Gir × Holstein crossbred.	
Thesaurus Nal: 	Genetic variance.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (24334-1) - UPC - AP - DD -  -  -  - DIS