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Registro Completo |
Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
Data corrente: |
03/03/2015 |
Data da última atualização: |
10/04/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LADINIG, A.; LUNNEY, J. K.; SOUZA, C. J. H.; ASHLEY, C.; PLASTOW, G.; HARDING, J. C. S. |
Afiliação: |
ANDREA LADINIG, UNIVERSITY OF SASKATCHEWAN, CANADA; JOAN K. LUNNEY, USDA; CARLOS JOSE HOFF DE SOUZA, CNPASA; CAROLYN ASHLEY, UNIVERSITY OF SASKATCHEWAN, CANADA; GRAHAM PLASTOW, UNIVERSITY OF ALBERTA, CANADA; JOHN C. S. HARDING, UNIVERSITY OF SASKATCHEWAN, CANADA. |
Título: |
Cytokine profiles in pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Veterinary Research, v. 45, 2014. |
DOI: |
10.1186/s13567-014-0113-8 |
Idioma: |
Inglês |
Conteúdo: |
In spite of extensive research, immunologic control mechanisms against Porcine Reproductive and Respiratory Syndrome virus (PRRSv) remain poorly understood. Cytokine responses have been exhaustively studied in nursery pigs and show contradictory results. Since no detailed reports on cytokine responses to PRRSv in pregnant females exist, the objectives of this study were to compare host cytokine responses between PRRSv-infected and non-infected pregnant gilts, and to investigate relationships between cytokine levels in infected gilts and viral load or fetal mortality rate. Serum samples and supernatants of peripheral blood mononuclear cells (PBMC) either stimulated with PRRSv or phorbol myristate acetate/Ionomycin (PMA/Iono) were analyzed for cytokines/chemokines: interleukins (IL) 1-beta (IL1β), IL4, IL8, IL10, IL12, chemokine ligand 2 (CCL2), interferon alpha (IFNα) and interferon gamma (IFNγ). Three cytokines (IFNα, CCL2, IFNγ) in gilt serum differed significantly in inoculated versus control gilts over time. In supernatants of PRRSv stimulated PBMC from PRRSv-infected gilts, levels of IFNα were significantly decreased, while IL8 secretion was significantly increased. PRRSv infection altered the secretion of all measured cytokines, with the exception of IFNα, from PBMC after mitogen stimulation, indicating a possible immunomodulatory effect of PRRSv. IFNα, CCL2, and IFNγ in serum, and IFNγ in supernatants of PMA/Iono stimulated PBMC were significantly associated with viral load in tissues, serum or both. However, only IFNα in supernatants of PRRSv stimulated PBMC was significantly associated with fetal mortality rate. We conclude that of the eight cytokines tested in this study IFNα was the best indicator of viral load and severity of reproductive PRRSv infection. MenosIn spite of extensive research, immunologic control mechanisms against Porcine Reproductive and Respiratory Syndrome virus (PRRSv) remain poorly understood. Cytokine responses have been exhaustively studied in nursery pigs and show contradictory results. Since no detailed reports on cytokine responses to PRRSv in pregnant females exist, the objectives of this study were to compare host cytokine responses between PRRSv-infected and non-infected pregnant gilts, and to investigate relationships between cytokine levels in infected gilts and viral load or fetal mortality rate. Serum samples and supernatants of peripheral blood mononuclear cells (PBMC) either stimulated with PRRSv or phorbol myristate acetate/Ionomycin (PMA/Iono) were analyzed for cytokines/chemokines: interleukins (IL) 1-beta (IL1β), IL4, IL8, IL10, IL12, chemokine ligand 2 (CCL2), interferon alpha (IFNα) and interferon gamma (IFNγ). Three cytokines (IFNα, CCL2, IFNγ) in gilt serum differed significantly in inoculated versus control gilts over time. In supernatants of PRRSv stimulated PBMC from PRRSv-infected gilts, levels of IFNα were significantly decreased, while IL8 secretion was significantly increased. PRRSv infection altered the secretion of all measured cytokines, with the exception of IFNα, from PBMC after mitogen stimulation, indicating a possible immunomodulatory effect of PRRSv. IFNα, CCL2, and IFNγ in serum, and IFNγ in supernatants of PMA/Iono stimul... Mostrar Tudo |
Thesagro: |
Porco; Reprodução animal; Respiração; Suino; Virus. |
Thesaurus Nal: |
Cytokines; Porcine reproductive and respiratory syndrome virus; Swine. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119182/1/cnpasa.pdf
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Marc: |
LEADER 02748naa a2200289 a 4500 001 2010493 005 2018-04-10 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1186/s13567-014-0113-8$2DOI 100 1 $aLADINIG, A. 245 $aCytokine profiles in pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome.$h[electronic resource] 260 $c2014 520 $aIn spite of extensive research, immunologic control mechanisms against Porcine Reproductive and Respiratory Syndrome virus (PRRSv) remain poorly understood. Cytokine responses have been exhaustively studied in nursery pigs and show contradictory results. Since no detailed reports on cytokine responses to PRRSv in pregnant females exist, the objectives of this study were to compare host cytokine responses between PRRSv-infected and non-infected pregnant gilts, and to investigate relationships between cytokine levels in infected gilts and viral load or fetal mortality rate. Serum samples and supernatants of peripheral blood mononuclear cells (PBMC) either stimulated with PRRSv or phorbol myristate acetate/Ionomycin (PMA/Iono) were analyzed for cytokines/chemokines: interleukins (IL) 1-beta (IL1β), IL4, IL8, IL10, IL12, chemokine ligand 2 (CCL2), interferon alpha (IFNα) and interferon gamma (IFNγ). Three cytokines (IFNα, CCL2, IFNγ) in gilt serum differed significantly in inoculated versus control gilts over time. In supernatants of PRRSv stimulated PBMC from PRRSv-infected gilts, levels of IFNα were significantly decreased, while IL8 secretion was significantly increased. PRRSv infection altered the secretion of all measured cytokines, with the exception of IFNα, from PBMC after mitogen stimulation, indicating a possible immunomodulatory effect of PRRSv. IFNα, CCL2, and IFNγ in serum, and IFNγ in supernatants of PMA/Iono stimulated PBMC were significantly associated with viral load in tissues, serum or both. However, only IFNα in supernatants of PRRSv stimulated PBMC was significantly associated with fetal mortality rate. We conclude that of the eight cytokines tested in this study IFNα was the best indicator of viral load and severity of reproductive PRRSv infection. 650 $aCytokines 650 $aPorcine reproductive and respiratory syndrome virus 650 $aSwine 650 $aPorco 650 $aReprodução animal 650 $aRespiração 650 $aSuino 650 $aVirus 700 1 $aLUNNEY, J. K. 700 1 $aSOUZA, C. J. H. 700 1 $aASHLEY, C. 700 1 $aPLASTOW, G. 700 1 $aHARDING, J. C. S. 773 $tVeterinary Research$gv. 45, 2014.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
15/08/2006 |
Data da última atualização: |
23/06/2009 |
Autoria: |
BARIONI, L. G.; FERREIRA, A. C.; RAMOS, A. K. B.; MARTHA JUNIOR, G. B.; SILVA, F. A. M.; VILELA, L.; VELOSO, R. F. |
Título: |
Planeamiento alimenticio y ajustes de la tasa de carga en estancias de ganadería bovina de carne. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
In: CONGRESO INTERNATIONAL DE TRANSFERENCIA DE TECNOLOGÍA AGROPECUARIA, 12., 2005, Mariano Roque Alonso, Paraguay. Praderas y forrajes. Mariano Roque Alonso: Asociación Rural del Paraguay, 2005. p. 111-134. |
Idioma: |
Espanhol |
Thesagro: |
Bovino; Carne. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00749naa a2200205 a 4500 001 1569624 005 2009-06-23 008 2005 bl uuuu u00u1 u #d 100 1 $aBARIONI, L. G. 245 $aPlaneamiento alimenticio y ajustes de la tasa de carga en estancias de ganadería bovina de carne. 260 $c2005 650 $aBovino 650 $aCarne 700 1 $aFERREIRA, A. C. 700 1 $aRAMOS, A. K. B. 700 1 $aMARTHA JUNIOR, G. B. 700 1 $aSILVA, F. A. M. 700 1 $aVILELA, L. 700 1 $aVELOSO, R. F. 773 $tIn: CONGRESO INTERNATIONAL DE TRANSFERENCIA DE TECNOLOGÍA AGROPECUARIA, 12., 2005, Mariano Roque Alonso, Paraguay. Praderas y forrajes. Mariano Roque Alonso: Asociación Rural del Paraguay, 2005. p. 111-134.
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