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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
05/09/1997 |
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Data da última atualização: |
11/12/2009 |
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Autoria: |
GUIMARAES, P. M. |
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Título: |
Development of a DNA probe and characterisation of Curtobacterium flaccumfaciens pv. flaccumfaciens. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
London: University of London, 1997. |
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Páginas: |
224p. |
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Idioma: |
Inglês |
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Notas: |
Ph.D. Thesis. |
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Conteúdo: |
Bacterial wilt of beans is a seedborne disease caused by the plant pathogenic coryneform bacteria Curtobacterium flaccumfaciens pv. flaccumfaciens. In order to construct a DNA probel for the identification of this organism, a chromosomal DNA library was built in the plasmid vector pGEM-7zf(+). The "Shot-gun" method was used for the construction of the DNA library in conjunction with a differential hybridisation technique. One of the clones (pPMP-26 D), after subsequent digestion with restriction endonucleases EcoRI/SacI, yielded a fragment of approximately 230 bp (pPMP-26 D) which hybridised specifically to C. f.pv. flaccumfaciens and not to any of the other eight-two coryneforms or bean pathogens tested in Southern, colony and dot-blots. Thisfragment was subcloned for use as a probe for the detection and identification of this organism. The uniqueness of the probe was confirmed by DNA sequencing. In addition, chemotaxonomic (fatty acid analysis) and genomic fingerpriting technique (ITS profiling, PCR of repetitive DNA and macrorestriction analysis by PFGE) were examined for the identification of profiles which allowed the differentiation of the pathovarswithin the species. The most discriminatory results were obtained by the use of pulsed-field gel electrophoresis analysis of HindIII and XbaI digests, Which produced markedly different patterns for each of the four C. |
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Palavras-Chave: |
Bean; Germoplasma: Patogeno: Identificação; Identifications. |
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Thesagro: |
Feijão; Phaseolus Vulgaris. |
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Thesaurus Nal: |
Curtobacterium flaccumfaciens; germplasm. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02023nam a2200217 a 4500
001 1051987
005 2009-12-11
008 1997 bl uuuu m 00u1 u #d
100 1 $aGUIMARAES, P. M.
245 $aDevelopment of a DNA probe and characterisation of Curtobacterium flaccumfaciens pv. flaccumfaciens.
260 $aLondon: University of London$c1997
300 $a224p.
500 $aPh.D. Thesis.
520 $aBacterial wilt of beans is a seedborne disease caused by the plant pathogenic coryneform bacteria Curtobacterium flaccumfaciens pv. flaccumfaciens. In order to construct a DNA probel for the identification of this organism, a chromosomal DNA library was built in the plasmid vector pGEM-7zf(+). The "Shot-gun" method was used for the construction of the DNA library in conjunction with a differential hybridisation technique. One of the clones (pPMP-26 D), after subsequent digestion with restriction endonucleases EcoRI/SacI, yielded a fragment of approximately 230 bp (pPMP-26 D) which hybridised specifically to C. f.pv. flaccumfaciens and not to any of the other eight-two coryneforms or bean pathogens tested in Southern, colony and dot-blots. Thisfragment was subcloned for use as a probe for the detection and identification of this organism. The uniqueness of the probe was confirmed by DNA sequencing. In addition, chemotaxonomic (fatty acid analysis) and genomic fingerpriting technique (ITS profiling, PCR of repetitive DNA and macrorestriction analysis by PFGE) were examined for the identification of profiles which allowed the differentiation of the pathovarswithin the species. The most discriminatory results were obtained by the use of pulsed-field gel electrophoresis analysis of HindIII and XbaI digests, Which produced markedly different patterns for each of the four C.
650 $aCurtobacterium flaccumfaciens
650 $agermplasm
650 $aFeijão
650 $aPhaseolus Vulgaris
653 $aBean
653 $aGermoplasma: Patogeno: Identificação
653 $aIdentifications
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Unidades Centrais. |
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Data corrente: |
13/12/2016 |
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Data da última atualização: |
03/08/2020 |
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Tipo da produção científica: |
Autoria/Organização/Edição de Livros |
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Autoria: |
VAZ JUNIOR, S. (ed.). |
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Afiliação: |
SILVIO VAZ JUNIOR, CNPAE. |
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Título: |
Analytical techniques and methods for biomass. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Cham: Springer, 2016. |
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Páginas: |
280 p. |
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Idioma: |
Inglês |
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Conteúdo: |
The use of analytical chemistry to understand biomass; qualitative and quantitative analysis of lignins from different sources and isolation methods for an application as a biobased chemical resources and polymeric material; Analyses of biomass fibers by XRD, FT-IR, and NIR; Molecular properties and functions of humic substances and humic-like substances (HULIS) from biomass and their transformation products; Mass spectrometry for metabolomics and biomass composition analyses; Nalyses of biomass products by nuclear magnetic rsonance spectroscopy; Microscopy applied in biomass characterization; Analytical strategies using chromatoraphic methodologies to analyze lignocellulosic feedstocks and their value-added compounds in biorefinery processes; Chemical analysis and characterization of biomass for biorefineries. |
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Palavras-Chave: |
Cadeias de biomassa; Composição química da biomassa; Impactos ambientais; Melhorias de produtos e processos. |
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Thesagro: |
Análise Química. |
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Thesaurus NAL: |
Analytical chemistry; Biomass; Chemical analysis; Metabolomics; Nuclear magnetic resonance spectroscopy. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01501nam a2200241 a 4500
001 2120787
005 2020-08-03
008 2016 bl uuuu 00u1 u #d
100 1 $aVAZ JUNIOR, S.
245 $aAnalytical techniques and methods for biomass.
260 $aCham: Springer$c2016
300 $a280 p.
520 $aThe use of analytical chemistry to understand biomass; qualitative and quantitative analysis of lignins from different sources and isolation methods for an application as a biobased chemical resources and polymeric material; Analyses of biomass fibers by XRD, FT-IR, and NIR; Molecular properties and functions of humic substances and humic-like substances (HULIS) from biomass and their transformation products; Mass spectrometry for metabolomics and biomass composition analyses; Nalyses of biomass products by nuclear magnetic rsonance spectroscopy; Microscopy applied in biomass characterization; Analytical strategies using chromatoraphic methodologies to analyze lignocellulosic feedstocks and their value-added compounds in biorefinery processes; Chemical analysis and characterization of biomass for biorefineries.
650 $aAnalytical chemistry
650 $aBiomass
650 $aChemical analysis
650 $aMetabolomics
650 $aNuclear magnetic resonance spectroscopy
650 $aAnálise Química
653 $aCadeias de biomassa
653 $aComposição química da biomassa
653 $aImpactos ambientais
653 $aMelhorias de produtos e processos
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Embrapa Unidades Centrais (AI-SEDE) |
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