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| Registros recuperados : 121 | |
| 81. |  | VAZ, C. S. L.; RECH, D. V.; REBELATTO, R.; DUARTE, S. C.; COLDEBELLA, A.; BESSA, M. C. Survival of Campylobacter jejuni in chicken at refrigeration and cooking temperatures. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 56, e02405, 2021.| Biblioteca(s): Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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| 82. | | VAZ, C. S. L.; RECH, D. V.; POZZA, J. dos S.; MATTOS, G. L. M.; COLDEBELLA, A.; SILVA, V. S. Campylobacter em frangos de corte: detecção laboratorial, dinâmica de colonização e caracterização. In: COLDEBELLA, A.; SCHEUERMANN, G.N. (Ed.). Relatório de projetos concluídos 2013. Concórdia: Embrapa Suínos e Aves, p. 15-28.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 83. | | ROSA, P. S.; AVILA, V. S. de; SILVA, V. S.; VAZ, C. S. L.; WILBERT, C. A.; RECH, D. V. Cama para frangos de corte. In: MACARI, M.; MENDES. A. A.; MENTEN, J. F.; NÄÄS, I. de A. Produção de frangos de corte. 2. ed. Campinas: FACTA, 2014. p.153-180.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 84. | | VAZ, C. S. L.; RECH, D. V.; MATTOS, G. L. M.; DUARTE, S. C.; KLEIN, C. S. Diversidade de Campylobacter termófilos de origem avícola no Sul do Brasil. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2014. 1 CD-ROM.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 85. | | VAZ, C. S. L.; DUARTE, S. C.; RECH, D. V.; REBELATTO, R.; COLDEBELLA, A.; BESSA, M. C. Efeito da temperatura sobre a viabilidade de campylobacter jejuni em carne de frango. In: CONFERÊNCIA APINCO DE CIÊNCIA E TECNOLOGIA AVÍCOLAS, 2016, Campinas, SP. Anais... Campinas: FACTA, 2016.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 87. | | RECH, D. V.; VAZ, C. S. L.; ALVES, L.; COLDEBELLA, A.; LEÃO. J. A.; RODRIGUES, D.; BACK, A. Caracterização fenotípica e genotípica de Salmonella spp. Isoladas de aviários de frangos de corte no Brasil entre 2009 e 2010. In: CONFERÊNCIA FACTA DE CIÊNCIA E TECNOLOGIA AVÍCOLAS, 2011, Santos, SP. Anais... Santos: FACTA, 2011. Trabalhos de Pesquisa José Maria Lamas da Silva. 1 CD-ROM. Projeto: 03.08.06.004.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 89. | | VAZ, C. S. L.; RECH, D. V.; DUARTE, S. C.; COLDEBELLA, A.; BESSA, M. C.; KLEIN, C. S. Microbial culture collection at Embrapa swine and poultry: promoting basic research on thermophilic campylobacter in chicken meat. In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA A AMÉRICA LATINA E CARIBE, 10., 2015, Bento Gonçalves. Recursos genéticos no século 21: de Vavilov a Svalbard: anais... [S.l.]: Sociedade Brasileira de Recursos Genéticos, 2015. p. 319.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 90. | | VAZ, C. S. L.; RECH, D. V.; FONSECA, F. M. da; MORES, M. A. Z.; COLDEBELLA, A. Redução de Salmonella Heidelberg em frangos pelo uso de bacteriófagos. Avicultura Industrial, Itu, ed. 1306, ano 112, n. 02, p. 16-18, 2021.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 92. | | RECH, D. V.; VAZ, C. S. L.; ALVES, L.; COLDEBELLA, A.; LEÃO, J. A.; RODRIGUES, D. dos P.; BACK, A. A temporal study of Salmonella enterica serotypes from broiler farms in Brazil. Poultry Science, v. 94, n. 3, p. 433-441, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 96. | | ALVES, L.; RECH, D. V.; GRITTI, D.; ESTEVES, W. T. C.; THOMÉ, J. D. S.; DUQUE, S. S.; FILGUEIRAS, A. L. L.; VAZ, C. S. L. Comparação de dois diferentes métodos de produção de atmosfera de microaerofilia para cultivo laboratorial de campylobacter termófilos In: JORNADA DE INICIAÇÃO CIENTÍFICA, 3., 2009, Concórdia. Anais... Concórdia: Embrapa Suínos e Aves/UNC, 2009. 1 CD-ROM Projeto/Plano de Ação: 03.08.60.400-02| Biblioteca(s): Embrapa Suínos e Aves. |
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| 97. | | BESSA, M. J.; HAAS, S.; RAMOS, S. C.; ROCHA, R.; RECH, D. V.; REBELATTO, R.; DUARTE, S. C.; COLDEBELLA, A.; VAZ, C. S. L. Survey of campylobacter in foods implicated in foodborne diseases in Southern Brazil. Revista do Instituto de Medicina Tropical de São Paulo, v. 62, artigo e90, 2020.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 98. | | VAZ, C. S. L.; RECH, D. V.; TREVISOL, I. M.; BRENTANO, L.; OKINO, C. H.; MORES, M. A. Z.; JAENISCH, F. R. F.; COLDEBELLA, A.; SILVA, V. S. Infectividade de micro-organismos em cama de frangos de corte submetida a diferentes tratamentos. Concórdia: Embrapa Suínos e Aves, 2017. 6 p. (Embrapa Suínos e Aves. Comunicado Técnico, 546).| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Suínos e Aves. |
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| 99. | | ALVES, L.; RECH, D. V.; GRITTI, D.; ESTEVES, W. T. C.; THOMÉ, J. D. S.; DUQUE, S. S.; FILGUEIRAS, A. A. L.; VAZ, C. S. L. Avaliação de métodos de produção de atmosfera de microaerofilia para isolamento de Campylobacter termófilos em fezes de galinhas. In: CONFERÊNCIA FACTA, 2010, Santos. Anais [dos] trabalhos de pesquisa José Maria Lamas da Silva Santos: FACTA, 2010. 1 CD-ROM. Projeto/Plano de Ação: 03.08.60.400-03.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 100. | | VAZ, C. S. L.; RECH, D. V.; POZZA, J. dos S.; SANTOS, F. B. de O.; COLDEBELLA, A.; SILVA, V. S. Dynamics of thermophilic campylobacter colonization in broiler flocks reared on reused litter. In: WORLD´S POULTRY CONGRESS, 24., 2012, Salvador. Abstract... Salvador: WSPA, 2012. 1 CD-ROM. Poultry Science Journal, v. 68, supl. 1, 2012. Projeto/Plano de Ação: 03.08.06.004.| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 121 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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Data corrente: |
11/04/2024 |
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Data da última atualização: |
11/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
VAZ, C. S. L.; FONSECA, F. M. da; RECH, D. V.; MORES, M. A. Z.; COLDEBELLA, A.; CANTAO, M. E. |
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Afiliação: |
CLARISSA SILVEIRA LUIZ VAZ, CNPSA; FRANCISCO NOE DA FONSECA, GEM; DAIANE VOSS RECH, CNPSA; MARCOS ANTONIO ZANELLA MORES, CNPSA; ARLEI COLDEBELLA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA. |
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Título: |
Wild-type lytic bacteriophages against Salmonella Heidelberg: further characterization and effect of prophylactic therapy in broiler chickens. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Research in Veterinary Science, v. 171, n. 105247, 2024. |
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DOI: |
https://doi.org/10.1016/j.rvsc.2024.105247 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous administration of the phage suspension most likely induced the development of bacteriophage-resistant mutants, which might have affected the in vivo effect. Therefore, a putative administration protocol should be based on other strategies, such as short-term therapy at pre-harvest age. MenosAbstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous a... Mostrar Tudo |
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Palavras-Chave: |
Análise genômica; Bacteriófago; Foodborne pathogen; Genomic analysis; Patógeno de origem alimentar; Terapia fágica. |
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Thesagro: |
Frango de Corte; Salmonelose. |
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Thesaurus NAL: |
Bacteriophages; Phage therapy; Poultry; Salmonellosis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02834naa a2200337 a 4500
001 2163526
005 2024-04-11
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.rvsc.2024.105247$2DOI
100 1 $aVAZ, C. S. L.
245 $aWild-type lytic bacteriophages against Salmonella Heidelberg$bfurther characterization and effect of prophylactic therapy in broiler chickens.$h[electronic resource]
260 $c2024
520 $aAbstract: To characterize wild-type bacteriophages and their effect on Salmonella Heidelberg intestinal colonization in broilers, phages combined in a cocktail were continuously delivered via drinking water since the first day after hatching. After challenge with a field strain, broilers were evaluated at regular intervals for S. Heidelberg and bacteriophages in tissues and cecum, and gross and microscopic lesions in organs. Phages were highly virulent against S. Heidelberg by efficiency of plating. One-step growth curves exhibited eclipse period from 20 to 25 min, whereas the lowest latent period and higher burst size found were 45 min and 54 PFU/cell, respectively. Bacteriophage whole genomic sequencing analyses revealed a lack of genes related to lysogeny, antimicrobial resistance, and virulence factors. Relevant gross or microscopic lesions were absent in tissues analyzed from treated broilers. Although numerically stable bacteriophage concentrations were detected in the cecal contents of treated broilers, no significant difference was found for the S. Heidelberg cecal load in comparison to the untreated group and for the prevalence of positive tissues throughout the evaluated period. The phages produced turbid plaques against some S. Heidelberg re-isolated from treated broilers, suggesting the evolving of a resistant subpopulation. Overall, the results provide new evidence of the safety and in vitro replication of such phages in S. Heidelberg. Nevertheless, continuous administration of the phage suspension most likely induced the development of bacteriophage-resistant mutants, which might have affected the in vivo effect. Therefore, a putative administration protocol should be based on other strategies, such as short-term therapy at pre-harvest age.
650 $aBacteriophages
650 $aPhage therapy
650 $aPoultry
650 $aSalmonellosis
650 $aFrango de Corte
650 $aSalmonelose
653 $aAnálise genômica
653 $aBacteriófago
653 $aFoodborne pathogen
653 $aGenomic analysis
653 $aPatógeno de origem alimentar
653 $aTerapia fágica
700 1 $aFONSECA, F. M. da
700 1 $aRECH, D. V.
700 1 $aMORES, M. A. Z.
700 1 $aCOLDEBELLA, A.
700 1 $aCANTAO, M. E.
773 $tResearch in Veterinary Science$gv. 171, n. 105247, 2024.
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