|
|
Registros recuperados : 161 | |
66. | | VALICENTE, F. H.; PAIVA, E.; VASCONCELOS, M. J. V. de; FIGUEIREDO, J. E. F. de; SANCHEZ, J.; BRAVO, A. Characterization by PCR analysis of cryl genes from Bacillus Thuringiensis strains efficient against fall armyworm, Spodoptera frugiperda. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 5., 1997, Singapore. Book of abstracts... Singapore: International Society for Plant Molecular Biology, 1997. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
67. | | VALICENTE, F. H.; PICOLI, E. A. de T.; VASCONCELOS, M. J. V. de; CARNEIRO, N.; CARNEIRO, A.; LANA, U. G. Caracterização, distribuição e clonagem de genes Cry em cepas de Bacillus thuringiensis do banco da Embrapa-CNPMS. In: CONGRESSO NACIONAL DE MILHO E SORGO, 27.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 3.; WORKSHOP SOBRE MANEJO E ETIOLOGIA DA MANCHA BRANCA DO MILHO, 2008, Londrina. Agroenergia, produção de alimentos e mudanças climáticas: desafios para milho e sorgo: trabalhos e palestras. [Londrina]: IAPAR; [Sete Lagoas]: Embrapa Milho e Sorgo, 2008. 1 CD-ROM. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
68. | | BARRETO, M. R.; PAIVA, E.; VASCONCELOS, M. J. V.; FARIA, A. L.; SOUZA, F. D. C.; VALICENTE, F. H. Caracterização de endotoxinas de cepas de Bacillus thuringiensis eficientes e não eficientes contra a lagarta do cartucho, Spodoptera frugiperda. In: SIMPOSIO DE CONTROLE BIOLOGICO, 6., 1998, Rio de Janeiro. Anais: sessoes de posters... Rio de Janeiro: FIOCRUZ, 1998. p. 169. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
69. | | VASCONCELOS, M. J. V. de; ANTUNES, M. S.; OLIVEIRA, M. F. de; LOPES, M. A.; FIGUEIREDO, J. E. F. Callus induction and plant regeneration from immature embryos culture of tropical maize. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 17, n. 3, p. 359-368, 2018. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
72. | | VALICENTE, F. H.; VASCONCELOS, M. J. V.; PAIVA, E.; FARIA, A. L.; SOUZA, F. D.; BARRETO, M. R. Caracterização através da PCR de cepas de Bacillus thuringiensis de diferentes regiões do Brasil. In: SIMPOSIO DE CONTROLE BIOLOGICO, 6., 1998, Rio de Janeiro. Anais: sessoes de posters... Rio de Janeiro: FIOCRUZ, 1998. p. 164. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
73. | | VASCONCELOS, M. J. V.; CARVALHO, C. H. S. de; PAIVA, E.; GUIMARAES, C. T.; MACHADO, M. A.; BARROS, E. G. Análise de RAPD em calos, plantas regeneradas e "seedlings" de milho (Zea mays L.). In: ENCONTRO BRASILEIRO DE BIOTECNOLOGIA VEGETAL, 1., 1993, Brasília. Programas e resumos. Brasília: EMBRAPA-CENARGEN, 1993. p. 45. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
75. | | SOUSA, S. M. de; PARENTONI, S. N.; GUIMARAES, C. T.; MAGALHAES, J. V. de; VASCONCELOS, M. J. V. de. Análise da morfologia do sistema radicular e da expressão de genes visando a associação com a eficiência na aquisição de fósforo em milho. In: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS, 2010. 1 CD-ROM. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
77. | | CARNEIRO, A. A.; CARNEIRO, N. P.; CARVALHO, C. H. S.; VASCONCELOS, M. J. V.; PAIVA, E.; LOPES, M. A. Milho transgênico: melhoria da qualidade nutricional do grão. Biotecnologia Ciencia & Desenvolvimento, Uberlandia, v. 3, n. 15, p. 42-46, 2000. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
80. | | RAGHOTHAMA, K. G.; KARTHIKEYAN, A. S.; JAIN, A.; RAMAIAH, M.; VASCONCELOS, M. J. V.; WAND, J.; CAO, A.; BALLACHANDA, D. Molecular regulation of phosphate starvation responses in plants. In: INTERNATIONAL SYMPOSIUM ON PHOSPHORUS DYNAMICS IN THE SOIL-PLANT CONTINUUM, 3., 2006, Uberlândia. Proceedings... Sete Lagoas: Embrapa Milho e Sorgo, 2006. p. 86. Biblioteca(s): Embrapa Milho e Sorgo. |
| |
Registros recuperados : 161 | |
|
|
Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
09/01/2019 |
Data da última atualização: |
12/02/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
VASCONCELOS, M. J. V. de; ANTUNES, M. S.; OLIVEIRA, M. F. de; LOPES, M. A.; FIGUEIREDO, J. E. F. |
Afiliação: |
MARIA JOSE VILACA DE VASCONCELOS, CNPMS; MAURICIO SCHUSTERSCHITZ ANTUNES, Colorado State University; MAURILIO FERNANDES DE OLIVEIRA, CNPMS; MAURICIO ANTONIO LOPES, DE/PR; JOSE EDSON FONTES FIGUEIREDO, CNPMS. |
Título: |
Callus induction and plant regeneration from immature embryos culture of tropical maize. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 17, n. 3, p. 359-368, 2018. |
Idioma: |
Inglês |
Conteúdo: |
The development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that the problem of the recalcitrant genotypes can be, at least partially, overcome by using immature embryos as explants together with tissue culture media formulations. Keywords: Zea mays, immature embryos, somatic embryogenesis, tissue culture, type I and type II calli. MenosThe development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that ... Mostrar Tudo |
Palavras-Chave: |
Calos tipo I e tipo II; Embriogênese somática. |
Thesagro: |
Cultura de Tecido; Embrião Vegetal; Zea Mays. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/190064/1/Callus-induction.pdf
|
Marc: |
LEADER 02500naa a2200229 a 4500 001 2103395 005 2019-02-12 008 2018 bl uuuu u00u1 u #d 100 1 $aVASCONCELOS, M. J. V. de 245 $aCallus induction and plant regeneration from immature embryos culture of tropical maize.$h[electronic resource] 260 $c2018 520 $aThe development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that the problem of the recalcitrant genotypes can be, at least partially, overcome by using immature embryos as explants together with tissue culture media formulations. Keywords: Zea mays, immature embryos, somatic embryogenesis, tissue culture, type I and type II calli. 650 $aCultura de Tecido 650 $aEmbrião Vegetal 650 $aZea Mays 653 $aCalos tipo I e tipo II 653 $aEmbriogênese somática 700 1 $aANTUNES, M. S. 700 1 $aOLIVEIRA, M. F. de 700 1 $aLOPES, M. A. 700 1 $aFIGUEIREDO, J. E. F. 773 $tRevista Brasileira de Milho e Sorgo, Sete Lagoas$gv. 17, n. 3, p. 359-368, 2018.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|