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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
26/02/2009 |
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Data da última atualização: |
06/07/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PIFFANELLI, P.; VILARINHOS, A. D.; SAFAR, J.; SABAU, X.; DOLEZEL, J. |
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Afiliação: |
Pietro Piffanelli, CIRAD-BIOS/Parco Tecnologico Padano; Alberto Duarte Vilarinhos, CNPMF; Jan Safar, Institute of Experimental Botany; Xavier Sabau, CIRAD-BIOS; Jaroslav Dolezel, Institute of Experimental Botany. |
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Título: |
Construction of bacterial artificial chromosome (BAC) libraries of banana (Musa acuminata and Musa balbisiana). |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Fruits, Paris, v. 63, n. 6, p. 1-5, 2008. |
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ISSN: |
0248-1294 |
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Idioma: |
Inglês |
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Conteúdo: |
Introduction. This protocol applies to the characterization of genome structure and evolution of M. acuminata and M. balbisiana genomes; construction of physical maps around markers of interest and QTLs; comparative genomic studies with other monocot species; and study of banana streak virus (BSV) integration patterns in banana nuclear genomes. The principle, key advantages, starting plant material, time required and expected results are presented. Materials and methods. This part describes the required materials, and the protocols for isolation and lysis of banana nuclei; high-molecular-weight (HMW) DNA Hind III digestion and pulsed-field gel electrophoresis (PFGE) analysis; large-scale HMW DNA Hind III digestion and ligation to the pINDIGOBAC-5 vector; and analysis of recombinant BAC clones. It mentions the main problems which can occur. Results. The described protocols will enable the efficient construction of BAC libraries from Musa species. These libraries can be used to construct physical maps and select specific genomic regions to be sequenced. |
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Palavras-Chave: |
Genetic map; Genetic marker; Method. |
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Thesagro: |
DNA; Musa sp. |
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Thesaurus Nal: |
electrophoresis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01765naa a2200253 a 4500
001 1655566
005 2023-07-06
008 2008 bl uuuu u00u1 u #d
022 $a0248-1294
100 1 $aPIFFANELLI, P.
245 $aConstruction of bacterial artificial chromosome (BAC) libraries of banana (Musa acuminata and Musa balbisiana).$h[electronic resource]
260 $c2008
520 $aIntroduction. This protocol applies to the characterization of genome structure and evolution of M. acuminata and M. balbisiana genomes; construction of physical maps around markers of interest and QTLs; comparative genomic studies with other monocot species; and study of banana streak virus (BSV) integration patterns in banana nuclear genomes. The principle, key advantages, starting plant material, time required and expected results are presented. Materials and methods. This part describes the required materials, and the protocols for isolation and lysis of banana nuclei; high-molecular-weight (HMW) DNA Hind III digestion and pulsed-field gel electrophoresis (PFGE) analysis; large-scale HMW DNA Hind III digestion and ligation to the pINDIGOBAC-5 vector; and analysis of recombinant BAC clones. It mentions the main problems which can occur. Results. The described protocols will enable the efficient construction of BAC libraries from Musa species. These libraries can be used to construct physical maps and select specific genomic regions to be sequenced.
650 $aelectrophoresis
650 $aDNA
650 $aMusa sp
653 $aGenetic map
653 $aGenetic marker
653 $aMethod
700 1 $aVILARINHOS, A. D.
700 1 $aSAFAR, J.
700 1 $aSABAU, X.
700 1 $aDOLEZEL, J.
773 $tFruits, Paris$gv. 63, n. 6, p. 1-5, 2008.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
03/05/2004 |
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Data da última atualização: |
11/07/2017 |
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Autoria: |
HUNGRIA, M.; NICOLÁS, M. F.; GUIMARAES, C. T.; JARDIM, S. N.; GOMES, E. A.; VASCONCELOS, A. T. T. de. |
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Afiliação: |
MARIANGELA HUNGRIA DA CUNHA, CNPSO; MARISA FABIANA NICOLÁS, Pós-graduanda; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; SILVIA NETO JARDIM BELICUAS, CNPMS; ELIANE APARECIDA GOMES, CNPMS; ANA TEREZA RIBEIRO DE VASCONCELOS, LNCC. |
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Título: |
Tolerance to stress and environmental adaptability of Chromobacterium violaceum. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 3, n. 1, p. 102-116, Mar. 2004. |
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ISSN: |
1676-5680 |
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Idioma: |
Inglês |
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Conteúdo: |
Chromobacterium violaceum is a Gram-negative bacterium, abundant in a variety of ecosystems in tropical and subtropical regions, including the water and borders of the Negro River, a major component of the Amazon Basin. As a free-living microorganism, C. violaceum is exposed to a series of variable conditions, such as different sources and abundance of nutrients, changes in temperature and pH, toxic compounds and UV rays. These variations, and the wide range of environments, require great adaptability and strong protective systems. The complete genome sequencing of this bacterium has revealed an enormous number and variety of ORFs associated with alternative pathways for energy generation, transport-related proteins, signal transduction, cell motility, secretion, and secondary metabolism. Additionally, the limited availability of iron in most environments can be overcome by iron-chelating compounds, iron-storage proteins, and by several proteins related to iron metabolism in the C. violaceum genome. Osmotically inducible proteins, transmembrane water-channel, and other membrane porins may be regulating the movement of water and maintaining the cell turgor, activities which play an important role in the adaptation to variations in osmotic pressure. Several proteins related to tolerance against antimicrobial compounds, heavy metals, temperature, acid and UV light stresses, others that promote survival under starvation conditions, and enzymes capable of detoxifying reactive oxygen species were also detected in C. violaceum. All these features together help explain its remarkable competitiveness and ability to survive under different types of environmental stresse. MenosChromobacterium violaceum is a Gram-negative bacterium, abundant in a variety of ecosystems in tropical and subtropical regions, including the water and borders of the Negro River, a major component of the Amazon Basin. As a free-living microorganism, C. violaceum is exposed to a series of variable conditions, such as different sources and abundance of nutrients, changes in temperature and pH, toxic compounds and UV rays. These variations, and the wide range of environments, require great adaptability and strong protective systems. The complete genome sequencing of this bacterium has revealed an enormous number and variety of ORFs associated with alternative pathways for energy generation, transport-related proteins, signal transduction, cell motility, secretion, and secondary metabolism. Additionally, the limited availability of iron in most environments can be overcome by iron-chelating compounds, iron-storage proteins, and by several proteins related to iron metabolism in the C. violaceum genome. Osmotically inducible proteins, transmembrane water-channel, and other membrane porins may be regulating the movement of water and maintaining the cell turgor, activities which play an important role in the adaptation to variations in osmotic pressure. Several proteins related to tolerance against antimicrobial compounds, heavy metals, temperature, acid and UV light stresses, others that promote survival under starvation conditions, and enzymes capable of detoxifying reactive oxy... Mostrar Tudo |
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Palavras-Chave: |
Adaptabilidade; Microorganismo; Tolerancia. |
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Thesagro: |
Bactéria; Stress. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02387naa a2200253 a 4500
001 1466991
005 2017-07-11
008 2004 bl uuuu u00u1 u #d
022 $a1676-5680
100 1 $aHUNGRIA, M.
245 $aTolerance to stress and environmental adaptability of Chromobacterium violaceum.
260 $c2004
520 $aChromobacterium violaceum is a Gram-negative bacterium, abundant in a variety of ecosystems in tropical and subtropical regions, including the water and borders of the Negro River, a major component of the Amazon Basin. As a free-living microorganism, C. violaceum is exposed to a series of variable conditions, such as different sources and abundance of nutrients, changes in temperature and pH, toxic compounds and UV rays. These variations, and the wide range of environments, require great adaptability and strong protective systems. The complete genome sequencing of this bacterium has revealed an enormous number and variety of ORFs associated with alternative pathways for energy generation, transport-related proteins, signal transduction, cell motility, secretion, and secondary metabolism. Additionally, the limited availability of iron in most environments can be overcome by iron-chelating compounds, iron-storage proteins, and by several proteins related to iron metabolism in the C. violaceum genome. Osmotically inducible proteins, transmembrane water-channel, and other membrane porins may be regulating the movement of water and maintaining the cell turgor, activities which play an important role in the adaptation to variations in osmotic pressure. Several proteins related to tolerance against antimicrobial compounds, heavy metals, temperature, acid and UV light stresses, others that promote survival under starvation conditions, and enzymes capable of detoxifying reactive oxygen species were also detected in C. violaceum. All these features together help explain its remarkable competitiveness and ability to survive under different types of environmental stresse.
650 $aBactéria
650 $aStress
653 $aAdaptabilidade
653 $aMicroorganismo
653 $aTolerancia
700 1 $aNICOLÁS, M. F.
700 1 $aGUIMARAES, C. T.
700 1 $aJARDIM, S. N.
700 1 $aGOMES, E. A.
700 1 $aVASCONCELOS, A. T. T. de
773 $tGenetics and Molecular Research$gv. 3, n. 1, p. 102-116, Mar. 2004.
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