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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
28/02/2000 |
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Data da última atualização: |
11/02/2015 |
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Autoria: |
STURION, J. A.; RESENDE, M. D. V. de; CARPANEZZI, A. A. |
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Afiliação: |
Pesquisadores da Embrapa Florestas. |
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Título: |
Controle genético e estimativa de ganho genético para peso de massa foliar em erva-Mate (Ilex paraguariensis St. Hil). |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Boletim de Pesquisa Florestal, Colombo, n. 38, p. 5-12, jan./jun. 1999. |
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ISSN: |
1517-6371 |
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Idioma: |
Português |
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Conteúdo: |
Verificou-se a magnitude da variabilidade genética, para massa foliar, em um teste de progênie de meios-irmãos de erva-mate (Ilex paraguariensis St. Hil), cujas sementes foram coletadas de ervais nativos na região de Toledo, PR. O teste foi instalado no município de Colombo, PR em dezembro de 1988, no delineamento de blocos casualizados com seis repetições e cinco plantas por parcela. Aos cinco anos e oito meses de idade, foram avaliados em cada árvore: o diâmetro da parte inferior da copa; altura e volume da copa e peso de massa foliar. A herdabilidade para peso de massa foliar, no sentido restrito, estimada ao nível de plantas, no experimento, foi da ordem de 20%. Assim, é necessário recorrer a métodos de seleção que utilizem simultaneamente as informações do indivíduo e da média de sua família, para melhorar o peso de massa foliar. O ganho genético estimado para peso de massa foliar (59,5%) foi bastante próximo daquele estimado, para essa variável, por meio do volume da copa (58,4%). Portanto, o volume da copa pode ser utilizado para seleção indireta do peso de massa foliar. |
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Palavras-Chave: |
Herdabilidade; Pomar de semente; Seed orchard; Selection. |
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Thesagro: |
Seleção; Variação Genética. |
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Thesaurus Nal: |
genetic variation; heritability. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPF-2009-09/4964/1/jsturion.pdf
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Marc: |
LEADER 01876naa a2200253 a 4500
001 1282204
005 2015-02-11
008 1999 bl uuuu u00u1 u #d
022 $a1517-6371
100 1 $aSTURION, J. A.
245 $aControle genético e estimativa de ganho genético para peso de massa foliar em erva-Mate (Ilex paraguariensis St. Hil).
260 $c1999
520 $aVerificou-se a magnitude da variabilidade genética, para massa foliar, em um teste de progênie de meios-irmãos de erva-mate (Ilex paraguariensis St. Hil), cujas sementes foram coletadas de ervais nativos na região de Toledo, PR. O teste foi instalado no município de Colombo, PR em dezembro de 1988, no delineamento de blocos casualizados com seis repetições e cinco plantas por parcela. Aos cinco anos e oito meses de idade, foram avaliados em cada árvore: o diâmetro da parte inferior da copa; altura e volume da copa e peso de massa foliar. A herdabilidade para peso de massa foliar, no sentido restrito, estimada ao nível de plantas, no experimento, foi da ordem de 20%. Assim, é necessário recorrer a métodos de seleção que utilizem simultaneamente as informações do indivíduo e da média de sua família, para melhorar o peso de massa foliar. O ganho genético estimado para peso de massa foliar (59,5%) foi bastante próximo daquele estimado, para essa variável, por meio do volume da copa (58,4%). Portanto, o volume da copa pode ser utilizado para seleção indireta do peso de massa foliar.
650 $agenetic variation
650 $aheritability
650 $aSeleção
650 $aVariação Genética
653 $aHerdabilidade
653 $aPomar de semente
653 $aSeed orchard
653 $aSelection
700 1 $aRESENDE, M. D. V. de
700 1 $aCARPANEZZI, A. A.
773 $tBoletim de Pesquisa Florestal, Colombo$gn. 38, p. 5-12, jan./jun. 1999.
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Registro original: |
Embrapa Florestas (CNPF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
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Data corrente: |
17/12/2014 |
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Data da última atualização: |
09/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL. |
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Título: |
Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Zygote, v. 23, n. 6, p. 852-862, 2015. |
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DOI: |
https://doi.org/10.1017/S0967199414000537 |
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Idioma: |
Inglês |
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Conteúdo: |
As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6–70.0% and blastocyst yield of 15.5–24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. MenosAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic deve... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Chemically induced enucleation; Microtubule; Nuclear transfer. |
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Thesaurus NAL: |
chromatin. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1018303/1/Chemically-induced-enucleation-of-activated-bovine-oocytes.pdf
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Marc: |
LEADER 02607naa a2200277 a 4500
001 2018303
005 2024-02-09
008 2015 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0967199414000537$2DOI
100 1 $aSARAIVA, N. Z.
245 $aChemically induced enucleation of activated bovine oocytes$bchromatin and microtubule organization and production of viable cytoplasts.$h[electronic resource]
260 $c2015
520 $aAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6–70.0% and blastocyst yield of 15.5–24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos.
650 $achromatin
653 $aBovine
653 $aChemically induced enucleation
653 $aMicrotubule
653 $aNuclear transfer
700 1 $aOLIVEIRA, C. S.
700 1 $aLEAL, C. L. V.
700 1 $aCALLADO, M. del
700 1 $aVANTINI, R.
700 1 $aMONTEIRO, F. M.
700 1 $aNICIURA, S. C. M.
700 1 $aGARCIA, J. M.
773 $tZygote$gv. 23, n. 6, p. 852-862, 2015.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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