Data/hora: 15/06/2024 02:59:12

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	16/02/2009	
Data da última atualização: 	07/07/2023	
Autoria: 	FERREIRA, C.; Van der LEE, T. A. J.; QUIROS, O.; HERRERT, B. L.; CARLIER, J.; TRUJILLO, C. D.; GOODWIN, S. B.; GUZMAN, M.; SOUZA, M.; KEMA, G. H. J.	
Afiliação: 	Cláudia Fortes Ferreira, CNPMF; Theo A. J. van der Lee, PRI-Plant Research International; Olman Quiros, PRI-Plant Research International; Bas L. Herrert, PRI-Plant Research International; Jean Carlier, PRI-Plant Research International; Caucasella D. Trujillo, PRI-Plant Research International; Stephen B. Goodwin, PRI-Plant Research International; Mauricio Guzman, PRI-Plant Research International; Manoel Souza, PRI-Plant Research International; Gert H. J. Kema, PRI-Plant Research International.	
Título: 	Development of cost effective tools for assessing genetic diversity of Mycosphaerella fijiensis, the causal agent of black-Sigatoka disease in bananas (Musa spp.).	
Ano de publicação: 	2008	
Fonte/Imprenta: 	In: EUROPEAN CONFERENCE ON FUNGAL GENETICS, 9., 2008, Edinburgh. Meeting abstracts... Edinburgh: University of Edinburgh, 2008.	
Idioma: 	Inglês	
Notas: 	PR9.29	
Conteúdo: 	Mycosphaerella fijiensis, the causal agent of banana leaf streak disease, commonly know as Black Sigatoka disease, is one of the most devastating plant pathogens. M. fijiensis is a relatively new pathogen and still actively colonizes new areas where bananas are grown. Using the genome sequence of M. fijiensis isolate CIRAD86 (http://www.genome.jgi-psf.org/Mycfi1/Mycfi1.home.html) we identified variable number of tandem repeat (VNTR) markers using a bioinformatics pipeline. The VNTR screening resulted in 1528 candidate VNTR loci of which many were imperfect mono - di - or tri - nucleotide repeats. Sixteen VNTR markers were selected based on repeat type, repeat length and the number of units of the repeat. Primers for these 16 VNTR markers were tested on a set of M. fijiensis isolates and five VNTR markers that showed multiple alleles were tested on progeny isolates and field isolates. The markers allowed robust scoring on agarose gels and proved useful for variability and population genetic studies using basic molecular instrumentation. We envisage that this set will allow more detailed analysis of the large scale migration pattern of this important pathogen and the small scale effect of fungicide tratments on the population diversity in banana plantations.	
Thesagro: 	Banana; Doença de Planta; Fungo; Genética Vegetal; Sigatoka Negra.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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