Data/hora: 15/06/2024 08:36:53

Biblioteca(s): | Embrapa Milho e Sorgo.
Data corrente: | 20/01/2020
Data da última atualização: | 20/01/2020
Autoria: | VALICENTE, F. H.
Afiliação: | FERNANDO HERCOS VALICENTE, CNPMS.
Título: | Entomophogenic viruses.
Ano de publicação: | 2019
Fonte/Imprenta: | In: SOUZA, B.; VÁZQUEZ, L. L.; MARUCCI, R. C. (Ed.). Natural enemies of insect pests in neotropical agroecosystems: biological control and functional biodiversity. Switzerland: Springer Nature, 2019.
Páginas: | p. 235-244.
DOI: | 10.1007/978-3-030-24733-1_20
Idioma: | Inglês
Conteúdo: | Although detailed descriptions of commercial production systems for baculovirus are scarce (Grzywacz et al. 2014), to achieve a successful in vivo baculovirus large- scale production, some important factors have to be taken into consideration. In general terms, baculovirus production can be divided into three parts: 1. Mass production of the healthy host. 2. Infection of the healthy host with baculovirus. 3. Harvest and/or storage and/or formulation of the infected host after its death. These three steps may or may not be taken at the same time, depending on the facility production. It also depends on the production goal of the industry, that is, if it is a small-, medium-, or large-scale production system. Baculovirus production is usually used for many species of Lepidoptera. To assure aseptic conditions, the room used for rearing healthy hosts needs to be separated from the infection and incubation rooms. There is no standard facility design; however, these rooms must be physically separated. All materials used in this room must be sterilized and kept in the room. Separating the host artificial rearing is a very important caution in the larvae production process, since it avoids undesired contamination of the healthy insects and of the colony. UV (ultraviolet) light bulbs may be used in every room. Although this irradiation is only effective for surface sterilization, this method may help keep the rooms clean. In case of colony contamination, the colony must be restarted with healthy larvae and sterilization of the room. If the necessary measures are not taken, the yield in this step of the process will be compromised. To achieve a good mass production of baculovirus-based biopesticides for commercial purposes, some important factors of the healthy host and cannibalistic behavior, as well as laboratory conditions, should be taken into consideration. This chapter details the mass production of baculovirus using healthy hosts. These hosts present both cannibalistic and noncannibalistic behaviors. For this experiment, we infected healthy hosts with baculovirus and inoculated a large number of hosts with the baculovirus. It is worth mentioning that the use of an alternative host and of harvesting and storing dead hosts is essential. We also list the equipment and materials needed in a baculovirus biofactory, the formulation, and quality control.
Palavras-Chave: | Biopesticida.
Thesagro: | Baculovirus; Produção.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Milho e Sorgo (CNPMS)
Exemplares: | CNPMS - (29101-1) - UPC - PL - DD -  -  -  - DIS