Data/hora: 02/06/2024 01:10:56

Biblioteca(s): 	Embrapa Agrobiologia.	
Data corrente: 	01/12/1997	
Data da última atualização: 	01/12/1997	
Autoria: 	BORNEMAN, J.; SKROCH, P. W.; O'SULLIVAN, K. M.; PALUS, J. A.; RUMJANEK, N. G.; JANSEN, J. L.; NIENHUIS, J.; TRIPLETT, E. W.	
Título: 	Molecular microbial diversity of an agricultural soil in Wisconsin.	
Ano de publicação: 	1996	
Fonte/Imprenta: 	Applied and Environmental Microbiology, Wisconsin, v. 62, n. 6, p. 1935-1943, 1996.	
Idioma: 	Inglês	
Conteúdo: 	A culture-independent survey of the soil microbial diversity in a clover-grass pasture in souther wisconsin was conducted by sequence analysis of a universal clone library of genes coding for small-subunit rRNA(rDNA). A rapid and efficient method for extraction of DNA from sils which resulted in highly purified DNA wit minimal shearing was developed. universal small-subunit-rRNA primers were used to amplify DNA extracted from the pasture soil. the PCR products were  cloned into pGEM-T,and either hypervariable or conseverd regions were sequenced. the relationships of 124 sequences to those of cultured orgnisms of known pylogeny were determined. of the 124 clones sequenced,98.4% were from the domain bacteria. two of the rDNA sequences were derived from eukaryotic organelles. two of the 124 sequences were of nuclear origin, one being fungal and the other a plant sequences. no sequences of the domain ARCHEA were found. within the domain bacteria,three kingdoms were highly representend:the PROTEOBACTERIA (16.1%),the CYTOPHAGA-FLEXIBACTER-BACTEROIDES GROUP(21.8%), and the low-g+c-contet gram-positive group(31.8%). some kingdoms,such as the thermotogales,the dreen nonsulfer groupthe fusobacteria,and the spirochaetes,were absent.a large number of the sequences(39.4%) were distributed among several clades that are not among the major taxa describ by olsen et al.(G.J.olsen,C.R.Woese,and Overbeek,J.Bacteriol.,176:1-6,1994).From the alignments of the sequencedata,distance matrices were calculated to display the enormous microbial diversity foud in this soil in two wals,as phylogenetic trees and as multidimencional-scaling plots.	
Thesagro: 	Ecologia; Microrganismo; Solo.	
Thesaurus Nal: 	microbial ecology; soil; Wisconsin.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agrobiologia (CNPAB)	
Exemplares: 	CNPAB - (24346-1) - UPC - AP - -- -  -  - 22187 - DIS