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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
29/03/2019 |
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Data da última atualização: |
21/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMARGO, L. S. de A.; MUNK, M.; SALES, J. N.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; VIANA, J. H. M. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MICHELE MUNK, UFJF; JOSE NELIO SALES, UFLA; SABINE WOHLRES-VIANA, UFJF; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Differential gene expression between in vivo and in vitro maturation: a comparative study with bovine oocytes derived from the same donor pool. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
JBRA Assisted Reproduction, v. 23, n. 1, p. 7-14, 2019. |
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DOI: |
10.5935/1518-0557.20180084 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Luiz Sergio Almeida Camargo. |
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Conteúdo: |
Objective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of polyadenylated transcripts in the oocyte cytoplasm when compared to in vivo maturation induced by exogenous hormones in oocytes collected from the same donor pool. MenosObjective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of p... Mostrar Tudo |
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Palavras-Chave: |
In vivo maturation; MRNA; Ovum pick-up. |
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Thesaurus Nal: |
Gene expression. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/195183/1/jbra-23-01-0007.pdf
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Marc: |
LEADER 02498naa a2200253 a 4500
001 2107759
005 2022-06-21
008 2019 bl uuuu u00u1 u #d
024 7 $a10.5935/1518-0557.20180084$2DOI
100 1 $aCAMARGO, L. S. de A.
245 $aDifferential gene expression between in vivo and in vitro maturation$ba comparative study with bovine oocytes derived from the same donor pool.$h[electronic resource]
260 $c2019
500 $aNa publicação: Luiz Sergio Almeida Camargo.
520 $aObjective: In vitro maturation has been shown to influence gene expression in oocytes, but a common shortcoming in reports on the matter has been the use of different donors in each experimental group thus disregarding donor effects. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo and in vitro obtained from the same group of donors. Methods: A bovine model was used to assess the relative abundance of specific transcripts in in vitro-matured (IN VITRO-OPU) and in vivo-matured (IN VIVO-OPU) oocytes collected from the same donors by transvaginal ovum pick-up (OPU). Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes in the IN VITRO-OPU group had lower (p<0.05) abundance of peroxiredoxin 1 (Prdx1), heat shock protein 70.1 (Hsp70.1), growth and differentiation factor 9 (Gdf9), and maternal antigen that embryo requires (Mater) transcripts than the oocytes in the IN VIVO-OPU group, all obtained from the same pool of donor cows. Similar results were seen in the comparisons involving the IN VIVO-OPU and IN VITRO-Abattoir groups (p<0.05). Conclusion: In vitro maturation affected the abundance of polyadenylated transcripts in the oocyte cytoplasm when compared to in vivo maturation induced by exogenous hormones in oocytes collected from the same donor pool.
650 $aGene expression
653 $aIn vivo maturation
653 $aMRNA
653 $aOvum pick-up
700 1 $aMUNK, M.
700 1 $aSALES, J. N.
700 1 $aWOHLRES-VIANA, S.
700 1 $aQUINTAO, C. C. R.
700 1 $aVIANA, J. H. M.
773 $tJBRA Assisted Reproduction$gv. 23, n. 1, p. 7-14, 2019.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos; Embrapa Semiárido. |
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Data corrente: |
07/12/2015 |
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Data da última atualização: |
21/12/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, B. S. G. DA; TORREZAN, R.; FREITAS, D. de G. C.; OLIVEIRA, A. H.; TEIXEIRA, N. S.; MATTOS, C. T. G. B.; FERREIRA, J. C. S.; SILVA, L. F. M. da; ARAUJO, F. P. de; MATTA, V. M. da. |
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Afiliação: |
UERJ; RENATA TORREZAN, CTAA; DANIELA DE GRANDI CASTRO FREITAS, CTAA; AGNELLI HOLANDA OLIVEIRA, CTAA; UNIVERSIDADE CASTELO BRANCO; CLAUDIA TORRES GOMES BRAUNS MATTOS, CTAA; JOSE CARLOS SA FERREIRA, CTAA; LUIZ FERNANDO MENEZES DA SILVA, CTAA; FRANCISCO PINHEIRO DE ARAUJO, CPATSA; VIRGINIA MARTINS DA MATTA, CTAA. |
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Título: |
Sensory evaluation of a Caatinga passion fruit based chutney. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 11., 2015, Campinas. Ciência de alimentos: qualidade de vida e envelhecimento saudáve. Campinas: SLACA: Unicamp, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was evaluate the acceptance of a chutney sauce based of the Caatinga passion fruit. |
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Palavras-Chave: |
Acceptance; Maracajá; Maracujá da Caatinga; Maracujá do mato; Melhoramento genético; Passiflora cincinnata mast; Preference test. |
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Thesagro: |
Mangifera Indica; Maracujá; Melhoramento Genético Vegetal. |
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Thesaurus NAL: |
Passion fruits. |
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Categoria do assunto: |
G Melhoramento Genético
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/135266/1/Pinheiro-2015-1.pdf
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Marc: |
LEADER 01256nam a2200349 a 4500
001 2031199
005 2023-12-21
008 2015 bl uuuu u00u1 u #d
100 1 $aSILVA, B. S. G. DA
245 $aSensory evaluation of a Caatinga passion fruit based chutney.$h[electronic resource]
260 $aIn: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 11., 2015, Campinas. Ciência de alimentos: qualidade de vida e envelhecimento saudáve. Campinas: SLACA: Unicamp$c2015
520 $aThe objective of this study was evaluate the acceptance of a chutney sauce based of the Caatinga passion fruit.
650 $aPassion fruits
650 $aMangifera Indica
650 $aMaracujá
650 $aMelhoramento Genético Vegetal
653 $aAcceptance
653 $aMaracajá
653 $aMaracujá da Caatinga
653 $aMaracujá do mato
653 $aMelhoramento genético
653 $aPassiflora cincinnata mast
653 $aPreference test
700 1 $aTORREZAN, R.
700 1 $aFREITAS, D. de G. C.
700 1 $aOLIVEIRA, A. H.
700 1 $aTEIXEIRA, N. S.
700 1 $aMATTOS, C. T. G. B.
700 1 $aFERREIRA, J. C. S.
700 1 $aSILVA, L. F. M. da
700 1 $aARAUJO, F. P. de
700 1 $aMATTA, V. M. da
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Registro original: |
Embrapa Semiárido (CPATSA) |
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