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| 4. |  | AMARAL, T. B.; MEDEIROS, S. R.; AURIEMO, A. J. B.; TORRES JÚNIOR, R. A. de A. Intervalo parto-primeiro cio, taxa de retorno ao cio, taxa de prenhez e colesterol plasmático de vacas Nelore primíparas submetidas a dois níveis de energia na dieta pós-parto. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 43., 2006, João Pessoa. Produção animal em biomas tropicais: anais. João Pessoa: Sociedade Brasileira de Zootecnia: UFPB, 2006. 4 p. 1 CD-ROM. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 10. | | BARICHELLO, F.; ALENCAR, M. M. de; TORRES JÚNIOR, R. A. de A. Estudo preliminar de análises de parâmetros genéticos em dados simulados de escores visuais com diferentes distribuições, por meio de inferência bayesiana. SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 7., 2008, São Carlos, SP. Anais... São Carlos, SP: SBMA: Embrapa Pecuária Sudeste, 2008. 4 p. 1 CD-ROM. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 13. | | BARICHELLO, F.; ALENCAR, M. M. de; TORRES JUNIOR, R. A. de A. Genetic analysis of visual score data with different distributions and genetic parameters using linear and nonlinear models. Journal of Animal Science, v. 88, (E-Suppl. 2), p. 699, 2010; Journal of Dairy Science, v. 93, (E-Suppl. 1), p. 699, 2010.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 15. | | SILVA, R. A.; MEDEIROS, S. R. de; REIS, S. F.; TORRES JUNIOR, R. A. de A. Teste de avaliação visual para taxa de acabamento de novilhas de corte. In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE,5., 2009, Campo Grande, MS. [Anais da ...]. Campo Grande, MS: Embrapa Gado de Corte, 2009. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
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| 20. | | MENEZES, G. R. de O.; TORRES JUNIOR, R. A. de A. Uso do cruzamento em gado de corte: o sucesso passa pela seleção. In: MENEZES, G. R. de O.; NOBRE, P. R. C.; TORRES JUNIOR, R. A. de A.; GONDO, A.; SILVA, L. O. C. da; SILVA, L. N. (Ed.). Sumário Senepol 2015: Sumário de touros Senepol Geneplus-Embrapa. Brasília, DF: Embrapa, 2015. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
26/12/2019 |
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Data da última atualização: |
13/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BLECHA, I. M. Z.; SOUSA, I. I.; FERREIRA, A. B. R.; FEIJO, G. L. D.; TORRES JUNIOR, R. A. de A.; SIQUEIRA, F. |
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Afiliação: |
Isabella Maiumi Zaidan Blecha, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal; Isadora Inácio Sousa, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia. Programa de Pós-Graduação em Ciência Animal; ANNA BEATRIZ ROBOTTOM FERREIRA, CTAA; GELSON LUIS DIAS FEIJO, CNPGC; ROBERTO AUGUSTO DE A TORRES JUNIOR, CNPGC; FABIANE SIQUEIRA, CNPGC. |
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Título: |
Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 48, e20180218, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform in any basic molecular biology laboratory. If the association of these markers with traits of economic interest in beef cattle is confirmed in new studies, these methodologies may contribute to the selection of animals with superior genetics, i.e., with the potential to produce better-quality meat, either by marker-assisted selection or by the inclusion of these polymorphisms in high-density marker panels. MenosThe objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform i... Mostrar Tudo |
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Thesaurus NAL: |
Animal breeding; Marker-assisted selection; Meat tenderness. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207815/1/Alternative-methodologies-for-genotyping.pdf
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|
Marc: |
LEADER 02589naa a2200217 a 4500
001 2117678
005 2020-01-13
008 2019 bl uuuu u00u1 u #d
100 1 $aBLECHA, I. M. Z.
245 $aAlternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle.$h[electronic resource]
260 $c2019
520 $aThe objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an experimental sample representative of the genetic variability in each breed. For the CAST and CAPN1 genes, respectively, the following number of animals were analyzed: Bonsmara (n = 25/22), Caracu (n = 25/26), Senepol (n = 25/24), Nelore (n = 26/26), and Angus (n = 25/24). The accuracy of these methodologies was confirmed by direct sequencing of PCR products generated for the two polymorphisms. The new primers developed for CAST/DdeI SNP detection and the use of DdeI enzyme for CAPN4751 SNP detection were effective in genotyping, since no inconclusive genotypes were observed for these genes. Thus, the genotyping of beef cattle using the PCR-RFLP technique for CAST and CAPN1 genes is robust, relatively inexpensive, and easy to perform in any basic molecular biology laboratory. If the association of these markers with traits of economic interest in beef cattle is confirmed in new studies, these methodologies may contribute to the selection of animals with superior genetics, i.e., with the potential to produce better-quality meat, either by marker-assisted selection or by the inclusion of these polymorphisms in high-density marker panels.
650 $aAnimal breeding
650 $aMarker-assisted selection
650 $aMeat tenderness
700 1 $aSOUSA, I. I.
700 1 $aFERREIRA, A. B. R.
700 1 $aFEIJO, G. L. D.
700 1 $aTORRES JUNIOR, R. A. de A.
700 1 $aSIQUEIRA, F.
773 $tRevista Brasileira de Zootecnia$gv. 48, e20180218, 2019.
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