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| Registros recuperados : 63 | |
| 8. |  | MORO, G. V.; RODRIGUES, A. P. O.; TORATI, L. S.; BARROSO, R. M.; LUNDSTEDT, L. M. Anatomia e fisiologia de peixes de água doce. In: RODRIGUES, A. P. O.; LIMA, A. F.; ALVES, A. L.; ROSA, D. K.; TORATI, L. S.; SANTOS, V. R. V. dos (Ed.). Piscicultura de água doce: multiplicando conhecimentos. Brasília, DF: Embrapa, 2013. p. 71-95. il.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 9. | | TORATI, L. S.; DE GRAVE, S.; PAGE, T. J.; ANKER, A. Atyidae and Palaemonidae (Crustacea: Decapoda: Caridea) of Bocas del Toro, Panama. Check List, São Paulo, v. 7, n. 6, p. 798-805, 2011.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 10. | | MORO, G. V.; TORATI, L. S.; LUIZ, D. de B.; MATOS, F. T. de. Monitoramento e manejo da qualidade da água em pisciculturas. In: RODRIGUES, A. P. O.; LIMA, A. F.; ALVES, A. L.; ROSA, D. K.; TORATI, L. S.; SANTOS, V. R. V. dos (Ed.). Piscicultura de água doce: multiplicando conhecimentos. Brasília, DF: Embrapa, 2013. p. 141-169. il.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 18. | | LIMA, A. F.; VARELA, E. S.; ALVES, R. R.; TORATI, L. S. Prospecção de medidas morfométricas do pirarucu em tamanho comercial. In: CONGRESSO BRASILEIRO DE ENGENHARIA DE PESCA, 17., 2011, Belém, PA. Engenharia de pesca: construindo o desenvolvimento sustentável do setor pesqueiro nacional: [anais]. Belém, PA: AEP: FAEP, 2011.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pesca e Aquicultura. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
|
Data corrente: |
20/10/2017 |
|
Data da última atualização: |
10/10/2019 |
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Autoria: |
TORATI, L. S. |
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Afiliação: |
LUCAS SIMON TORATI, CNPASA. |
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Título: |
Reproductive physiology of Arapaima gigas (Schinz, 1822) and development of tools for broodstock management. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
2017 |
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Páginas: |
322 f. |
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Idioma: |
Inglês |
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Notas: |
Tese (Doutorado) - Institute of Aquaculture, University of Stirling, Escócia. |
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Conteúdo: |
Arapaima gigas is the largest scaled freshwater fish in the world reaching over 250 kg. With growth rates of 10 kg+ within 12 months, A. gigas is considered as a promising
candidate species for aquaculture development in South America. However, the lack of reproductive control in captivity is hindering the industry expansion. The work carried
out in this doctoral thesis therefore aimed to better understand the species? reproductive physiology, develop tools to identify gender and monitor gonad development, test
hormonal therapies to induce ovulation and spawning and characterise the cephalic secretion for its potential roles in pheromone release and during parental care. Initially, a
genomic study investigated the overall extent of polymorphism in A. gigas, which was found to be surprisingly low, with only 2.3 % of identified RAD-tags (135 bases long)
containing SNPs. Then, a panel with 293 single nucleotide polymorphism (SNP) was used to characterise the genetic diversity and structure of a range of Amazon populations.
Results revealed populations from the Amazon and Solimões appeared to be genetically different from the Araguaia population, while Tocantins population comprised individuals from both stocks. This data provided a tool for broodstock identification and future management. The PhD then aimed to evaluate the effects of slow-release mGnRH implants and different broodstock size pairings on maturation and spawning. Results showed that the implants stimulated the brain-pituitary-gonad axis resulting in increased plasma levels of testosterone (females) and 11-ketotestosterone in males, respectively regardless of pairing sizes. However, no spawning was observed. Results also showed the release of sex steroids with potential pheromonal action through the cephalic secretion, a biological fluid released from the adult head along the reproductive period. Thereafter, a non-surgical field endoscopy method was developed and validated for ovarian assessment and gender identification. The method was then used to describe the female gonopore and obtain biopsy of the ovary through cannulation which allowed the description of oogenesis in A. gigas. Importantly, oocytes obtained by cannulation confirmed that adult females under investigation were maturing with oocytes in final maturation stage but failed to ovulate/spawn. Another hormonal induction trial was therefore performed in which a combination of GnRHa (mGnRHa/sGnRHa) was used by injection to induce ovulation and spawning in selected maturing females with effects on oocyte maturation monitored post-induction through biopsy. However, this trial appeared to not be successful at inducing ovulation or spawning. Finally, the peptidome and proteome of the cephalic secretion was further characterised through the comparison between parental and non-parental fish. Results highlighted the complex role of this biological fluid including potential roles on the developing offspring during the parental care period. Overall, this doctoral thesis provided new basic and applied data on A. gigas reproduction and tools that can be used in future studies to better understand the environmental and hormonal control of oogenesis and spawning. MenosArapaima gigas is the largest scaled freshwater fish in the world reaching over 250 kg. With growth rates of 10 kg+ within 12 months, A. gigas is considered as a promising
candidate species for aquaculture development in South America. However, the lack of reproductive control in captivity is hindering the industry expansion. The work carried
out in this doctoral thesis therefore aimed to better understand the species? reproductive physiology, develop tools to identify gender and monitor gonad development, test
hormonal therapies to induce ovulation and spawning and characterise the cephalic secretion for its potential roles in pheromone release and during parental care. Initially, a
genomic study investigated the overall extent of polymorphism in A. gigas, which was found to be surprisingly low, with only 2.3 % of identified RAD-tags (135 bases long)
containing SNPs. Then, a panel with 293 single nucleotide polymorphism (SNP) was used to characterise the genetic diversity and structure of a range of Amazon populations.
Results revealed populations from the Amazon and Solimões appeared to be genetically different from the Araguaia population, while Tocantins population comprised individuals from both stocks. This data provided a tool for broodstock identification and future management. The PhD then aimed to evaluate the effects of slow-release mGnRH implants and different broodstock size pairings on maturation and spawning. Results showed that the implants stimulated the bra... Mostrar Tudo |
|
Palavras-Chave: |
Araguaia; Solimões. |
|
Thesagro: |
Arapauma gigas; Criação em cativeiro; Peixe de água doce; Pirarucu; Piscicultura; Polimorfismo genético; Recurso genético; Reprodução. |
|
Thesaurus NAL: |
Amazonia. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 04030nam a2200265 a 4500
001 2077716
005 2019-10-10
008 2017 bl uuuu m 00u1 u #d
100 1 $aTORATI, L. S.
245 $aReproductive physiology of Arapaima gigas (Schinz, 1822) and development of tools for broodstock management.$h[electronic resource]
260 $a2017$c2017
300 $a322 f.
500 $aTese (Doutorado) - Institute of Aquaculture, University of Stirling, Escócia.
520 $aArapaima gigas is the largest scaled freshwater fish in the world reaching over 250 kg. With growth rates of 10 kg+ within 12 months, A. gigas is considered as a promising candidate species for aquaculture development in South America. However, the lack of reproductive control in captivity is hindering the industry expansion. The work carried out in this doctoral thesis therefore aimed to better understand the species? reproductive physiology, develop tools to identify gender and monitor gonad development, test hormonal therapies to induce ovulation and spawning and characterise the cephalic secretion for its potential roles in pheromone release and during parental care. Initially, a genomic study investigated the overall extent of polymorphism in A. gigas, which was found to be surprisingly low, with only 2.3 % of identified RAD-tags (135 bases long) containing SNPs. Then, a panel with 293 single nucleotide polymorphism (SNP) was used to characterise the genetic diversity and structure of a range of Amazon populations. Results revealed populations from the Amazon and Solimões appeared to be genetically different from the Araguaia population, while Tocantins population comprised individuals from both stocks. This data provided a tool for broodstock identification and future management. The PhD then aimed to evaluate the effects of slow-release mGnRH implants and different broodstock size pairings on maturation and spawning. Results showed that the implants stimulated the brain-pituitary-gonad axis resulting in increased plasma levels of testosterone (females) and 11-ketotestosterone in males, respectively regardless of pairing sizes. However, no spawning was observed. Results also showed the release of sex steroids with potential pheromonal action through the cephalic secretion, a biological fluid released from the adult head along the reproductive period. Thereafter, a non-surgical field endoscopy method was developed and validated for ovarian assessment and gender identification. The method was then used to describe the female gonopore and obtain biopsy of the ovary through cannulation which allowed the description of oogenesis in A. gigas. Importantly, oocytes obtained by cannulation confirmed that adult females under investigation were maturing with oocytes in final maturation stage but failed to ovulate/spawn. Another hormonal induction trial was therefore performed in which a combination of GnRHa (mGnRHa/sGnRHa) was used by injection to induce ovulation and spawning in selected maturing females with effects on oocyte maturation monitored post-induction through biopsy. However, this trial appeared to not be successful at inducing ovulation or spawning. Finally, the peptidome and proteome of the cephalic secretion was further characterised through the comparison between parental and non-parental fish. Results highlighted the complex role of this biological fluid including potential roles on the developing offspring during the parental care period. Overall, this doctoral thesis provided new basic and applied data on A. gigas reproduction and tools that can be used in future studies to better understand the environmental and hormonal control of oogenesis and spawning.
650 $aAmazonia
650 $aArapauma gigas
650 $aCriação em cativeiro
650 $aPeixe de água doce
650 $aPirarucu
650 $aPiscicultura
650 $aPolimorfismo genético
650 $aRecurso genético
650 $aReprodução
653 $aAraguaia
653 $aSolimões
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