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| 49. |  | MOURA, L. M. de; THOMAS, D. W. Impactos das mudanças de tecnologia, na produção e nas rendas do gado leiteiro em Viçosa, M.G. Experientiae, Viçosa, MG, v. 8, n. 2, p. 25-89, jun. 1968. il.| Biblioteca(s): Embrapa Amazônia Oriental. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
19/04/1999 |
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Data da última atualização: |
19/04/1999 |
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Autoria: |
HUDDLESTON, A. S.; CRESSWELL, N.; NEVES, M. C. P.; BERINGER, J. E.; BAUMBERG, S.; THOMAS, D. I.; WELLINGTON, E. M. H. |
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Título: |
Molecular detection of streptomycin-producing streptomycetes in Brazilian soils. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 63, n. 4, p. 1288-1297, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
Actinomycetes were isolated from soybean rhizosphere soil collected at two field sites in Brazil. All the isolates were identified as Streptomyces species and were screened for streptomycin production and the presence of two genes, strA and strB1, known to be involved in streptomycin biosynthesis in Streptomyces griseus. Antibiotic resistance profiles were determined for 53 isolates from cultivated and uncultivated sites, and approximately half the strains wre streptomycin resistant. Clustering by the unweighted pair group method with averages indicated the presence of two major clusters, with the majority of resistant strains from cultivated sites being placed in cluster 1. Only representatives from this cluster contained strA. Streptomycetes containing strA and strB1 were phenotypically diverse, and only half could be assigned to known species. Sequence comparison of 16S rRNA and trpBA (tryptophan synthetase) genes revealed that streptomycin-producing streptomycetes were phylogenetically diverse. It appeared that a population of streptomycetes had colonized the rhizosphere and that a proportion of these were capable of streptomycin production. |
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Palavras-Chave: |
Brasil; Production; Resistance to chemicals. |
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Thesagro: |
Actinomiceto; Antibiótico; Estreptomicina; Gene; Produção; Resistência a Produtos Químicos; Rizosfera; Soja; Solo. |
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Thesaurus NAL: |
Actinomycetales; antibiotics; Brazil; genes; rhizosphere; soil; soybeans; Streptomyces griseus; streptomycin. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02337naa a2200445 a 4500
001 1621025
005 1999-04-19
008 1997 bl uuuu u00u1 u #d
100 1 $aHUDDLESTON, A. S.
245 $aMolecular detection of streptomycin-producing streptomycetes in Brazilian soils.
260 $c1997
520 $aActinomycetes were isolated from soybean rhizosphere soil collected at two field sites in Brazil. All the isolates were identified as Streptomyces species and were screened for streptomycin production and the presence of two genes, strA and strB1, known to be involved in streptomycin biosynthesis in Streptomyces griseus. Antibiotic resistance profiles were determined for 53 isolates from cultivated and uncultivated sites, and approximately half the strains wre streptomycin resistant. Clustering by the unweighted pair group method with averages indicated the presence of two major clusters, with the majority of resistant strains from cultivated sites being placed in cluster 1. Only representatives from this cluster contained strA. Streptomycetes containing strA and strB1 were phenotypically diverse, and only half could be assigned to known species. Sequence comparison of 16S rRNA and trpBA (tryptophan synthetase) genes revealed that streptomycin-producing streptomycetes were phylogenetically diverse. It appeared that a population of streptomycetes had colonized the rhizosphere and that a proportion of these were capable of streptomycin production.
650 $aActinomycetales
650 $aantibiotics
650 $aBrazil
650 $agenes
650 $arhizosphere
650 $asoil
650 $asoybeans
650 $aStreptomyces griseus
650 $astreptomycin
650 $aActinomiceto
650 $aAntibiótico
650 $aEstreptomicina
650 $aGene
650 $aProdução
650 $aResistência a Produtos Químicos
650 $aRizosfera
650 $aSoja
650 $aSolo
653 $aBrasil
653 $aProduction
653 $aResistance to chemicals
700 1 $aCRESSWELL, N.
700 1 $aNEVES, M. C. P.
700 1 $aBERINGER, J. E.
700 1 $aBAUMBERG, S.
700 1 $aTHOMAS, D. I.
700 1 $aWELLINGTON, E. M. H.
773 $tApplied and Environmental Microbiology, Washington$gv. 63, n. 4, p. 1288-1297, 1997.
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