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Registros recuperados : 4 | |
1. | | SANTOS, A. C. C. dos; HAMMES, V. S.; LOPES, D. B.; VILELA FILHO, O.; SAMPAIO, M. J. A. M.; NALERIO, E. S.; DINIZ, F. H.; KIILL, L. H. P.; SILVA, M. S. L. da; GAMBETTA, R.; ARZABE, C.; PIEROZZI JUNIOR, I. Contextualização dos Objetivos de Desenvolvimento Sustentável no Brasil e na Embrapa. In: HAMMES, V. S.; LOPES, D. B.; SANTOS, A. C. C. dos; COSTA, J. R. da; OLIVEIRA, Y. M. M. de (Ed.). Pesquisa e inovação agropecuária na agenda 2030: contribuições da Embrapa e parceiros. Brasília, DF: Embrapa, 2018. E-book. Cap. 1. 21-39 (Objetivos de Desenvolvimento Sustentável, 18) Ação Gerencial
Agenda 2030; ODS; ODS Embrapa; Rede ODS Embrapa; Objetivos de Desenvolvimento Sustentável; Sustentabilidade Embrapa; ODS Publicação Biblioteca(s): Embrapa Agroenergia; Embrapa Gado de Leite; Embrapa Pecuária Sul; Embrapa Semiárido; Embrapa Solos; Embrapa Uva e Vinho. |
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2. | | SANTOS, A. C. C. dos; HAMMES, V. S.; LOPES, D. B.; VILELA FILHO, O.; SAMPAIO, M. J. A. M.; NALERIO, E. S.; DINIZ, F. H.; KIILL, L. H. P.; SILVA, M. S. L. da; GAMBETTA, R.; ARZABE, C.; PIEROZZI JUNIOR, I. Contextualizing sustainable development goals in Brazil and Embrapa. In: HAMMES, V. S.; LOPES, D. B.; SANTOS, A. C. C. dos; COSTA, J. R. da; OLIVEIRA, Y. M. M. de (Ed.). Agricultural research and innovation in the 2030 Agenda: contributions of Embrapa and partners. Brasília, DF: Embrapa, 2021. Cap.1. p. 13-23 E-book. (Sustainable development goal, 18). Translated by Paulo de Holanda Morais. Biblioteca(s): Embrapa Agricultura Digital; Embrapa Agroenergia; Embrapa Gado de Leite; Embrapa Pecuária Sul; Embrapa Semiárido; Embrapa Solos; Embrapa Unidades Centrais; Embrapa Uva e Vinho. |
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3. | | ARZABE, C.; COSTA, J. R. da; LOPES, D. B.; NALERIO, E. S.; ANTUNES, E.; DINIZ, F. H.; FOGACA, F. H. dos S.; MOZZER, G. B.; ARAUJO, G. P. de; ALMEIDA, J. S. S. E.; NOGUEIRA, J. D.; MELLO, L. M. R. de; KIILL, L. H. P.; FREITAS, M. H. A. de; SAMPAIO, M. J. A. M.; SILVA, M. S. L. da; COSTA, P. da; MELO, P. E. de; GAMBETTA, R.; DIAS, T. A. B.; OLIVEIRA, Y. M. M. de; BUENO, Y. M.; HAMMES, V. S.; SANTOS, A. C. C. dos; PIEROZZI JUNIOR, I.; SILVA, A. C. da; ALARCAO, A. L. L.; REIS, A. E. G. dos; TORRES, D. A. P.; CONTINI, E.; PRADO, H. A. do; COSTA, J. L. da S.; DUARTE, J. A. M.; VILELA FILHO, O.; NASCIMENTO, P. P.; MENEZES, R. A. de L.; RAMOS, R. G. C.; BELTRAO, S. L. L.; ARAUJO, S. C. B. de; PEREIRA, V. da F. Contributions of Embrapa to the 5 Ps: people, prosperity, planet, partnership and peace. In: HAMMES, V. S.; LOPES, D. B.; SANTOS, A. C. C. dos; COSTA, J. R. da; OLIVEIRA, Y. M. M. de (Ed.). Agricultural research and innovation in the 2030 Agenda: contributions of Embrapa and partners. Brasília, DF: Embrapa, 2021. Cap. 2. p. 25-44. (Sustainable development goals, 18). Biblioteca(s): Embrapa Agricultura Digital; Embrapa Agroindústria de Alimentos; Embrapa Amazônia Ocidental; Embrapa Florestas; Embrapa Gado de Leite; Embrapa Meio Ambiente; Embrapa Pecuária Sul; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido; Embrapa Solos; Embrapa Unidades Centrais; Embrapa Uva e Vinho. MenosEmbrapa Agricultura Digital; Embrapa Agroindústria de Alimentos; Embrapa Amazônia Ocidental; Embrapa Florestas; Embrapa Gado de Leite; Embrapa Meio Ambiente; Embrapa Pecuária Sul; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido; Embrapa Solos... Mostrar Todas |
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4. | | ARZABE, C.; COSTA, J. R. da; LOPES, D. B.; NALERIO, E. S.; ANTUNES, E.; DINIZ, F. H.; FOGACA, F. H. dos S.; MOZZER, G. B.; ARAUJO, G. P. de; ALMEIDA, J. S. S. E.; NOGUEIRA, J. D.; MELLO, L. M. R. de; KIILL, L. H. P.; FREITAS, M. H. A. de; SAMPAIO, M. J. A. M.; SILVA, M. S. L. da; COSTA, P. da; MELO, P. E. de; GAMBETTA, R.; DIAS, T. A. B.; OLIVEIRA, Y. M. M. de; BUENO, Y. M.; HAMMES, V. S.; SANTOS, A. C. C. dos; PIEROZZI JUNIOR, I.; SILVA, A. C. da; ALARCAO, A. L. L.; REIS, A. E. G. dos; TORRES, D. A. P.; CONTINI, E.; PRADO, H. A. do; COSTA, J. L. da S.; DUARTE, J. A. M.; VILELA FILHO, O.; NASCIMENTO, P. P.; MENEZES, R. A. de L.; RAMOS, R. G. C.; BELTRAO, S. L. L.; ARAUJO, S. C. B. de; PEREIRA, V. da F. Contribuições da Embrapa para os 5 Ps: pessoas, prosperidade, planeta, parceria e paz. In: HAMMES, V. S.; LOPES, D. B.; SANTOS, A. C. C. dos; COSTA, J. R. da; OLIVEIRA, Y. M. M. de (Ed.). Pesquisa e inovação agropecuária na agenda 2030: contribuições da Embrapa e parceiros. Brasília, DF: Embrapa, 2018. E-book. Cap. 2. p. 40-79. (Objetivos de Desenvolvimento Sustentável, 18). Biblioteca(s): Embrapa Agricultura Digital; Embrapa Agroenergia; Embrapa Agroindústria de Alimentos; Embrapa Amazônia Ocidental; Embrapa Florestas; Embrapa Gado de Leite; Embrapa Pecuária Sul; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Roraima; Embrapa Semiárido; Embrapa Solos; Embrapa Uva e Vinho. MenosEmbrapa Agricultura Digital; Embrapa Agroenergia; Embrapa Agroindústria de Alimentos; Embrapa Amazônia Ocidental; Embrapa Florestas; Embrapa Gado de Leite; Embrapa Pecuária Sul; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Roraima; Embrapa Semiárido... Mostrar Todas |
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Registros recuperados : 4 | |
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Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
25/05/2009 |
Data da última atualização: |
15/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; GARCIA, J. M. |
Afiliação: |
NAIARA ZOCCAL SARAIVA, UNESP/JABOTICABAL; FELIPE PERECIN, FZEA/USP; SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTINA RAMIRES FERREIRA, FZEA/USP; TATIANA ALMEIDA DRUMOND TETZNER, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
Título: |
Demecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Cloning and Stem Cells, v. 11, n. 1, p. 141-152, mar. 2009. |
DOI: |
10.1089/clo.2008.0044 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst development (27.6%) rates were assessed. In conclusion, demecolcine can be used at lower concentrations than routinely employed, and the chemically assisted enucleation technique was proven to be highly efficient allowing embryonic development in bovine. MenosThis study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst ... Mostrar Tudo |
Thesaurus NAL: |
oocytes. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02377naa a2200205 a 4500 001 1048984 005 2023-03-15 008 2009 bl uuuu u00u1 u #d 024 7 $a10.1089/clo.2008.0044$2DOI 100 1 $aSARAIVA, N. Z. 245 $aDemecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes.$h[electronic resource] 260 $c2009 520 $aThis study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst development (27.6%) rates were assessed. In conclusion, demecolcine can be used at lower concentrations than routinely employed, and the chemically assisted enucleation technique was proven to be highly efficient allowing embryonic development in bovine. 650 $aoocytes 700 1 $aPERECIN, F. 700 1 $aMÉO, S. C. 700 1 $aFERREIRA, C. R. 700 1 $aTETZNER, T. A. D. 700 1 $aGARCIA, J. M. 773 $tCloning and Stem Cells$gv. 11, n. 1, p. 141-152, mar. 2009.
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