|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
18/04/2024 |
Data da última atualização: |
18/04/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, E. R. dos; CAMARGO, B. R. de; SILVA, L. A. da; LAUMANN, R. A.; RIBEIRO, B. M.; ARDISSON-ARAÚJO, D. M. P. |
Afiliação: |
ETHIANE ROZO DOS SANTOS, UNIVERSIDADE DE BRASÍLIA; BRENDA RABELO DE CAMARGO, UNIVERSIDADE DE BRASÍLIA; LEONARDO ASSIS DA SILVA, UNIVERSIDADE DE BRASÍLIA; RAUL ALBERTO LAUMANN, CENARGEN; BERGMANN MORAIS RIBEIRO, UNIVERSIDADE DE BRASÍLIA; DANIEL M. P. ARDISSON-ARAÚJO, UNIVERSIDADE DE BRASÍLIA. |
Título: |
The multispecies stinkbug iflavirus Halyomorpha halys virus detected in the multispecies stinkbug egg parasitoid microwasp, Telenomus podisi (Ashmead) (Hymenoptera: Platygastridae). |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, 2024. |
DOI: |
https://doi.org/10.1007/s42770-024-01340-y |
Idioma: |
Português |
Notas: |
On-line first. |
Conteúdo: |
Wasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological significance of HhV remain elusive, warranting further studies to illuminate both the transmission process and its capacity for environmental propagation using T. podisi as a potential vector. MenosWasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological si... Mostrar Tudo |
Palavras-Chave: |
Egg parasitoid; Halyomorpha halys virus; Stinkbug; Telenomus podisi. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02558naa a2200253 a 4500 001 2163741 005 2024-04-18 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s42770-024-01340-y$2DOI 100 1 $aSANTOS, E. R. dos 245 $aThe multispecies stinkbug iflavirus Halyomorpha halys virus detected in the multispecies stinkbug egg parasitoid microwasp, Telenomus podisi (Ashmead) (Hymenoptera$bPlatygastridae).$h[electronic resource] 260 $c2024 500 $aOn-line first. 520 $aWasps are important parasitoids of stinkbugs and frequently exposed to various types of microorganisms through environmental contact and fecal–oral transmission route. Many parasitize stinkbug eggs and are commercially used in the field to control insect population. The parasitoid T. podisi is known for its high parasitism capacity and ability to target multiple species of stinkbugs. In this study we asked whether T. podisi exposed to eggs infected by a multispecies asymptomatic stinkbug virus, the Halyomorpha halys virus (HhV) would get infected. HhV is a geographically distributed multispecies iflavirus previously found to infect four stinkbug hosts, including three Brazilian species, Chinavia ubica, Euschistus heros and Diceraeus melacanthus, and T. posidi can parasitize all of them. As results, RT-PCR screening revealed positive samples for the HhV genome in two out of four tested pools of T. podisi, whereas the antigenome, indicative of replicative activity, was not detected. The wasps were raised in E. heros eggs that presented both the genome and the antigenome forms of the HhV genome. Subsequent RNA-deep sequencing of HhV positive T. podisi RNA pools yielded a complete genome of HhV with high coverage. Phylogenetic analysis positioned the isolate HhV-Tp (isolate Telenomus podisi) alongside with the stinkbug HhV. Analysis of transcriptomes from several hymenopteran species revealed HhV-Tp reads in four species. However, the transmission mechanism and the ecological significance of HhV remain elusive, warranting further studies to illuminate both the transmission process and its capacity for environmental propagation using T. podisi as a potential vector. 653 $aEgg parasitoid 653 $aHalyomorpha halys virus 653 $aStinkbug 653 $aTelenomus podisi 700 1 $aCAMARGO, B. R. de 700 1 $aSILVA, L. A. da 700 1 $aLAUMANN, R. A. 700 1 $aRIBEIRO, B. M. 700 1 $aARDISSON-ARAÚJO, D. M. P. 773 $tBrazilian Journal of Microbiology, 2024.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
13/05/2010 |
Data da última atualização: |
16/01/2019 |
Tipo da produção científica: |
Folder/Folheto/Cartilha |
Autoria: |
TESSMANN, D. J.; SOUZA, R. T. de; CAPELARI, S. |
Afiliação: |
Dauri José Tessmann, Universidade Estadual de Maringá; REGINALDO TEODORO DE SOUZA, CNPUV; Silvia Capelari, Emater Marialva. |
Título: |
Monitoramento de condições climáticas: uma ferramenta para o manejo do míldio da videira no Norte do Paraná. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Bento Gonçalves: Embrapa Uva e Vinho, 2010. |
Descrição Física: |
1 folder. |
Idioma: |
Português |
Palavras-Chave: |
Aplicação; Calendário; Molhamento foliar; Monitoramento. |
Thesagro: |
Agrotóxico; Clima; Doença de planta; Fungicida; Manejo; Míldio; Uva; Viticultura. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/110713/1/Reginaldo-monitoramentoParana.pdf
|
Marc: |
LEADER 00756nam a2200277 a 4500 001 1783601 005 2019-01-16 008 2010 bl uuuu u0uu1 u #d 100 1 $aTESSMANN, D. J. 245 $aMonitoramento de condições climáticas$buma ferramenta para o manejo do míldio da videira no Norte do Paraná. 260 $aBento Gonçalves: Embrapa Uva e Vinho$c2010 300 $c1 folder. 650 $aAgrotóxico 650 $aClima 650 $aDoença de planta 650 $aFungicida 650 $aManejo 650 $aMíldio 650 $aUva 650 $aViticultura 653 $aAplicação 653 $aCalendário 653 $aMolhamento foliar 653 $aMonitoramento 700 1 $aSOUZA, R. T. de 700 1 $aCAPELARI, S.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|