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Registros recuperados : 41 | |
4. | | TEIXEIRA, M. F. da S.; LAMBERT, V.; MSELLI-LAKAHL, L.; CHETTAB, A.; CHEBLOUNE, Y.; MORNEX, J. F. Immortalization of caprine fibroblasts permissive for replication of small ruminant lentiviruses. American Journal of Veterinary Research, v.58, n.6, p.579-584, 1997. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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5. | | SILVA, D. F.; PEIXOTO, R. M.; ARAÚJO, J. F.; DAMASCENO, E. M.; TEIXEIRA, M. F. da S.; ANDRIOLI, A. Avaliação comparativa de diluidores na manutenção dos parâmetros seminais de caprinos. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 5., 2016, Sobral. Anais... Sobral: Embrapa Caprinos e Ovinos, 2016. p. 29-30. [ALICE ANDRIOLI PINHEIRO]. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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8. | | RICARTE, A. R. F.; TEIXEIRA, M. F. da S.; ANDRIOLI, A.; PINHEIRO, R. R.; BÁO, S. N.; SILVA, J. B. A. da. Análise molecular e ultraestrutural de espermatozóides caprinos oriundos de animais infectados naturalmente e experimentalmente com o CAEV. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 6.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 7.; FÓRUM DE COORDENADORES DE PÓS GRADUAÇÃO EM PRODUÇÃO ANIMAL DO NORDESTE, 1.; FÓRUM DE AGROECOLOGIA RO RIO GRANDE DO NORTE, 1., 2010, Mossoró. Anais... Mossoró: Sociedade Nordestina de Producao Animal; UFERSA, 2010. 4 f. 1 CD-ROM. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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9. | | RODRIGUES, A. P. R.; PINTO, M. T.; TEIXEIRA, M. F. da S.; MELO, A. C. M.; FREITAS, V. J. de F. Análise macro e microscópica das lesões em caprinos soropositivos para o vírus da artrite-encefalite caprina sem a presença de sinais clínicos. Ciência Animal, Fortaleza, v. 11, n. 2, p. 113-118, 2001. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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10. | | GÓES, C. D. M. de M. B.; TEIXEIRA, M. F. da S.; ALVES, F. S. F.; BATISTA, L. M.; BEVILAQUA, C. M. L. Avaliação da transferência passiva de anticorpos em cabritos alimentados com três diferentes tipos de colostro. Ciência Animal, Fortaleza, v. 8, n. 2, p. 57-61, 1998. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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11. | | FERREIRA, R. C. S.; TEIXEIRA, M. F. da S.; ROCHA, M. F. G.; LIMA, S. M. C.; FROTA, M. N. L. da; MELO, A. C. M. Redução da população microbiana presente no leite de cabra submetido à temperatura de 56oC por 60 minutos. Ciência Animal, Fortaleza, v. 11, n. 1, p. 39-44, 2001. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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12. | | SOUZA, K. C. de; ANDRIOLI, A.; SIDER, L. H.; PINHEIRO, R. R.; BEZERRA JUNIOR, R. Q.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. Detecção de sequências do DNA proviral do vírus da Artrite Encefalite Caprina em saliva. Acta Scientiae Veterinariae, v. 43, n. 1266, p. 1-6, 2015. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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13. | | PEIXOTO, R. M.; TEIXEIRA, M. F. da S.; ANDRIOLI, A.; PINHEIRO, R. R.; SILVA, A. A. de S.; AGUIAR, T. D. de F.; SOUSA, A. L. M. de. Perspectivas do uso de fitoterápicos no tratamento do sêmen caprino infectado pelo vírus da Artrite Encefalite Caprina. Medicina Veterinária (UFRPE), v. 12, n. 3, p. 193-201, jul/set. 2018. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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14. | | AZEVEDO, D. A. A. de; SANTOS, V. W. S. dos; SOUSA, A. L. M. de; PEIXOTO, R. M.; PINHEIRO, R. R.; ANDRIOLI, A.; TEIXEIRA, M. F. da S. Small ruminant lentiviruses: economic and productive losses, consequences of the disease. Arquivos do Instituto Biológico, v. 84, p. 1-10, e0552016, 2017. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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15. | | PAULA, N. R. de O.; ANDRIOLI, A.; CARDOSO, J. de F. S.; PINHEIRO, R. R.; SOUSA, F. M. L.; SOUZA, K. C. de; ALVES, F. S. F.; CAMPELLO, C. C.; RICARTE, A. R. F.; TEIXEIRA, M. F. da S. Profile of the Caprine arthritis-encephalitis virus (CAEV) in blood, semen from bucks naturally and experimentally infected in the Semi-arid region of Brazil. Small Ruminant Research, v. 85, n. 1, p. 27-33, Jul. 2009. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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16. | | PEIXOTO, R. M.; TEIXEIRA, M. F. da S.; ANDRIOLI, A.; PINHEIRO, R. R.; ALVES, F. S. F.; SANTOS, V. W. S. dos; SOUSA, M. M. de; AMARAL, G. R. Prevalência sorológica de Mycoplasma agalactiae em rebanhos de caprinos leiteiros na mesorregião metropolitana de Fortaleza - CE. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 12., 2017, Juazeiro, BA. Construindo pontes entre o ensino, a pesquisa e a extensão: anais. Petrolina: Univasf: Embrapa Semiárido: Instituto Federal de Educação, Ciência e Tecnologia, Sertão de Pernambuco, 2017. 1 CD-ROM. p. 1284-1286. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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17. | | ARAÚJO, S. A. C. de; TEIXEIRA, M. F. da S.; DANTAS, T. V. M.; MIRANDA, A. M.; LIMA, F. E. S.; MELO, V. S. P. de; RICARTE, A. R. F.; COSTA, E. C. Avaliação in vitro da atividade citotóxica de drogas antivirais em fibroblastos caprinos. Ciência Animal, Fortaleza, v. 18, n. 1 p. 25-31, 2008. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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18. | | PEIXOTO, R. M.; ANDRIOLI, A.; SANTOS, D. O.; PINHEIRO, R. R.; ARAÚJO, J. F.; SOUSA, A. L. M. de; SILVA, D. F.; DAMASCENO, E. M.; TEIXEIRA, M. F. da S. Avaliação da toxicidade de solvente de extratos vegetais com ação antiviral em sêmen caprino refrigerado. Acta Scientiae Veterinariae, v. 45, n. 1487, p. 1-8, 2017. [ALICE ANDRIOLI PINHEIRO]. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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19. | | BEZERRA JUNIOR, R. Q.; ELOY, A. M. X.; PEREIRA, E. P.; FURTADO, J. R.; SOUZA, K. C. de; LIMA, A. R.; PINHEIRO, R. R.; ANDRIOLI, A.; TEIXEIRA, M. F. da S. Avaliação das metaloproteinases de matriz no sangue de reprodutores caprinos naturalmente infectados com Artrite Encefalite Caprina na Região Semiárida do Brasil. Acta Scientiae Veterinariae, v. 43, pub. 1258, p. 1-7, 2015. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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20. | | AZEVEDO, D. A. A. de; MONTEIRO, J. P.; PINHEIRO, R. R.; MUDADU, M. de A.; ANDRIOLI, A.; ARAÚJO, J. F.; SOUSA, A. L. M. de; SIDER, L. H.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. Molecular characterization of circulating strains of small ruminant lentiviruses in Brazil based on complete gag and pol genes. Small Ruminant Research, v. 177, p. 160-166, 2019. Biblioteca(s): Embrapa Agricultura Digital; Embrapa Caprinos e Ovinos. |
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Registros recuperados : 41 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Caprinos e Ovinos. |
Data corrente: |
27/09/2019 |
Data da última atualização: |
07/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
AZEVEDO, D. A. A. de; MONTEIRO, J. P.; PINHEIRO, R. R.; MUDADU, M. de A.; ANDRIOLI, A.; ARAÚJO, J. F.; SOUSA, A. L. M. de; SIDER, L. H.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. |
Afiliação: |
DALVA ALANA ARAGÃO DE AZEVEDO, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; JOMAR PATRICIO MONTEIRO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; MAURICIO DE ALVARENGA MUDADU, CNPTIA; ALICE ANDRIOLI, CNPC; JUSCILÂNIA FURTADO ARAÚJO, Northeast Biotechnology Network, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; ANA LÍDIA MADEIRA DE SOUSA, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; LUCIA HELENA SIDER, CNPC; RENATO MESQUITA PEIXOTO, Universidade Federal do Acre (UFAC) - Rio Branco, AC, Brazil; MARIA FÁTIMA DA SILVA TEIXEIRA, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil. |
Título: |
Molecular characterization of circulating strains of small ruminant lentiviruses in Brazil based on complete gag and pol genes. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Small Ruminant Research, v. 177, p. 160-166, 2019. |
DOI: |
https://doi.org/10.1016/j.smallrumres.2019.06.011 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Small ruminant lentiviruses (SRLV) are widely distributed in the world and cause productive and economic losses. These pathogens have high genetic variability, which hinders diagnosis by molecular methods. Molecular characterization studies are good strategies for elaborating programs aimed to control or eradicate diseases. Hence, this study aimed to sequence, characterize and compare strains of SRLV, which were isolated from naturally infected goats from the states of Ceará, Rio Grande do Norte and Minas Gerais, Brazil, based on total nucleotide sequences of gag and pol genes. SRLV isolates from the microbiological collection of Sheep and Goat of Embrapa were cultured in goat synovial membrane. Supernatants containing cells were stored and viral particles were purified with sucrose density gradient centrifugation for RNA extraction. Then, sequencing in HiSeq 2500 platform (Illumina) was performed to obtain the total genome of the SRLV strains. The software MEGA version 7.0 was used for sequence editing and phylogeny analysis. A total of 17 isolates were multiplied in cellular culture and six were viable, which were two from Minas Gerais (MG), one from Rio Grande do Norte (RN) and three from Ceará (CE). Two sequences were obtained from the sequencing by synthesis, one from MG (BRMG CNPC) and one from RN (BRRN CNPC). From the total SRLV genome (9,200 bases), sample BRMG CNPC generated a consensus of 6,378 bases, approximately 69,34% of the viral genome and sample BRRN CNPC generated a consensus of 5,351 bases, which comprised 58.16% of the total genome. Samples in this study demonstrated closer phylogenetic relation with genotype B (B1 group). The remaining strains sequenced in Brazil are also present in the same group with the exception of three samples from genotype A. Immunodominant regions of gag gene and motifs of pol gene were similar to the standard CAEV Cork strain. For the first time, the complete gag and pol genes were sequenced in Brazil from the strain BRMG CNPC isolated in Minas Gerais state. In addition, 2490 bases from the total of 3316 bases were sequenced from the pol gene of the BRRN CNPC sample from Rio Grande do Norte. MenosAbstract: Small ruminant lentiviruses (SRLV) are widely distributed in the world and cause productive and economic losses. These pathogens have high genetic variability, which hinders diagnosis by molecular methods. Molecular characterization studies are good strategies for elaborating programs aimed to control or eradicate diseases. Hence, this study aimed to sequence, characterize and compare strains of SRLV, which were isolated from naturally infected goats from the states of Ceará, Rio Grande do Norte and Minas Gerais, Brazil, based on total nucleotide sequences of gag and pol genes. SRLV isolates from the microbiological collection of Sheep and Goat of Embrapa were cultured in goat synovial membrane. Supernatants containing cells were stored and viral particles were purified with sucrose density gradient centrifugation for RNA extraction. Then, sequencing in HiSeq 2500 platform (Illumina) was performed to obtain the total genome of the SRLV strains. The software MEGA version 7.0 was used for sequence editing and phylogeny analysis. A total of 17 isolates were multiplied in cellular culture and six were viable, which were two from Minas Gerais (MG), one from Rio Grande do Norte (RN) and three from Ceará (CE). Two sequences were obtained from the sequencing by synthesis, one from MG (BRMG CNPC) and one from RN (BRRN CNPC). From the total SRLV genome (9,200 bases), sample BRMG CNPC generated a consensus of 6,378 bases, approximately 69,34% of the viral genome and sample BR... Mostrar Tudo |
Palavras-Chave: |
Caracterização molecular; Molecular characterization; SRLV. |
Thesaurus NAL: |
Genome; Goats; Lentivirus; Phylogeny; Sheep. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 03206naa a2200337 a 4500 001 2112529 005 2019-11-07 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.smallrumres.2019.06.011$2DOI 100 1 $aAZEVEDO, D. A. A. de 245 $aMolecular characterization of circulating strains of small ruminant lentiviruses in Brazil based on complete gag and pol genes.$h[electronic resource] 260 $c2019 520 $aAbstract: Small ruminant lentiviruses (SRLV) are widely distributed in the world and cause productive and economic losses. These pathogens have high genetic variability, which hinders diagnosis by molecular methods. Molecular characterization studies are good strategies for elaborating programs aimed to control or eradicate diseases. Hence, this study aimed to sequence, characterize and compare strains of SRLV, which were isolated from naturally infected goats from the states of Ceará, Rio Grande do Norte and Minas Gerais, Brazil, based on total nucleotide sequences of gag and pol genes. SRLV isolates from the microbiological collection of Sheep and Goat of Embrapa were cultured in goat synovial membrane. Supernatants containing cells were stored and viral particles were purified with sucrose density gradient centrifugation for RNA extraction. Then, sequencing in HiSeq 2500 platform (Illumina) was performed to obtain the total genome of the SRLV strains. The software MEGA version 7.0 was used for sequence editing and phylogeny analysis. A total of 17 isolates were multiplied in cellular culture and six were viable, which were two from Minas Gerais (MG), one from Rio Grande do Norte (RN) and three from Ceará (CE). Two sequences were obtained from the sequencing by synthesis, one from MG (BRMG CNPC) and one from RN (BRRN CNPC). From the total SRLV genome (9,200 bases), sample BRMG CNPC generated a consensus of 6,378 bases, approximately 69,34% of the viral genome and sample BRRN CNPC generated a consensus of 5,351 bases, which comprised 58.16% of the total genome. Samples in this study demonstrated closer phylogenetic relation with genotype B (B1 group). The remaining strains sequenced in Brazil are also present in the same group with the exception of three samples from genotype A. Immunodominant regions of gag gene and motifs of pol gene were similar to the standard CAEV Cork strain. For the first time, the complete gag and pol genes were sequenced in Brazil from the strain BRMG CNPC isolated in Minas Gerais state. In addition, 2490 bases from the total of 3316 bases were sequenced from the pol gene of the BRRN CNPC sample from Rio Grande do Norte. 650 $aGenome 650 $aGoats 650 $aLentivirus 650 $aPhylogeny 650 $aSheep 653 $aCaracterização molecular 653 $aMolecular characterization 653 $aSRLV 700 1 $aMONTEIRO, J. P. 700 1 $aPINHEIRO, R. R. 700 1 $aMUDADU, M. de A. 700 1 $aANDRIOLI, A. 700 1 $aARAÚJO, J. F. 700 1 $aSOUSA, A. L. M. de 700 1 $aSIDER, L. H. 700 1 $aPEIXOTO, R. M. 700 1 $aTEIXEIRA, M. F. da S. 773 $tSmall Ruminant Research$gv. 177, p. 160-166, 2019.
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